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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Clostridium botulinum (strain 657 / Type Ba4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411SubstrateUniRule annotation
Binding sitei142 – 1421SubstrateUniRule annotation
Metal bindingi171 – 1711Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi215 – 2151Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi270 – 2701Divalent metal cation; shared with dimeric partnerUniRule annotation
Binding sitei278 – 2781SubstrateUniRule annotation
Binding sitei296 – 2961SubstrateUniRule annotation

GO - Molecular functioni

  1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD binding Source: InterPro

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
  2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, NADP

Enzyme and pathway databases

BioCyciCBOT515621:GCP3-2386-MONOMER.
UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Ordered Locus Names:CLJ_B2416
OrganismiClostridium botulinum (strain 657 / Type Ba4)
Taxonomic identifieri515621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002333 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3343344-hydroxythreonine-4-phosphate dehydrogenasePRO_1000211910Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi515621.CLJ_B2416.

Structurei

3D structure databases

ProteinModelPortaliC3KYY3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221593.
KOiK00097.
OMAiTHGDING.
OrthoDBiEOG6GN6ZC.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

C3KYY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINNKPIIGI PIGDPAGVGP EIVVKSLTQA EIYEKCNPIL IGDAKVIKQA
60 70 80 90 100
MGFCNVNLNI NSIKKVDEGK FTLGTIDLID LNNIDIDELK IGKVQGIAGK
110 120 130 140 150
AAFEYIKKSV EMAKEGELDA IATTPINKES LREGNVNYIG HTEILADLTD
160 170 180 190 200
TEDPLTMFEV RGMRVFFLTR HVSLRKACDL VTKERVLDYI IRCSEALEKL
210 220 230 240 250
GVKDGKMAVA GLNPHSGEHG LFGDEEMKAV VPAIEEAQKM GYKVEGPIGA
260 270 280 290 300
DSVFHLALKG RYNSVLSLYH DQGHIATKTL DFERTIAVTN GMPILRTSVD
310 320 330
HGTAFDIAGT GQASSVSMVE AIILAAKYSP KFKK
Length:334
Mass (Da):36,314
Last modified:June 16, 2009 - v1
Checksum:i6E373CC452F25B1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ54394.1.
RefSeqiYP_002863183.1. NC_012658.1.

Genome annotation databases

EnsemblBacteriaiACQ54394; ACQ54394; CLJ_B2416.
KEGGicbi:CLJ_B2416.
PATRICi19413183. VBICloBot19908_2621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ54394.1.
RefSeqiYP_002863183.1. NC_012658.1.

3D structure databases

ProteinModelPortaliC3KYY3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi515621.CLJ_B2416.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ54394; ACQ54394; CLJ_B2416.
KEGGicbi:CLJ_B2416.
PATRICi19413183. VBICloBot19908_2621.

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221593.
KOiK00097.
OMAiTHGDING.
OrthoDBiEOG6GN6ZC.

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.
BioCyciCBOT515621:GCP3-2386-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Clostridium botulinum Ba4 strain 657."
    Shrivastava S., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 657 / Type Ba4.

Entry informationi

Entry nameiPDXA_CLOB6
AccessioniPrimary (citable) accession number: C3KYY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: April 1, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.