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Protein

NAD kinase

Gene

nadK

Organism
Clostridium botulinum (strain 657 / Type Ba4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei61Proton acceptorUniRule annotation1
Binding sitei145NADUniRule annotation1
Binding sitei164NADUniRule annotation1
Binding sitei234NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 62NADUniRule annotation2
Nucleotide bindingi134 – 135NADUniRule annotation2
Nucleotide bindingi175 – 180NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:CLJ_B2066
OrganismiClostridium botulinum (strain 657 / Type Ba4)
Taxonomic identifieri515621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002054121 – 281NAD kinaseAdd BLAST281

Structurei

3D structure databases

ProteinModelPortaliC3KXC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000227222.
KOiK00858.
OMAiMCHFEIA.
OrthoDBiPOG091H02D1.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

C3KXC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIGININT DKDISRNILD KIFQYIHEEC SEAKIKVFYD SKGLDNEESR
60 70 80 90 100
ALDAVMVLGG DGTILGTARA LAKYDVPIFG INRGHLGFLA EIELEDCKKA
110 120 130 140 150
IKNLFKGQYK IEDRIMLKCD LKGIDKKDDF LALNDIVLTK GNLSRIVKYS
160 170 180 190 200
IYVDDVWYTT FVADGVIVAT PTGSTAYSLS AGGPIVYPDL DVLEIAPICP
210 220 230 240 250
HSLGIRPILL NGNSKINIRV LKKYEDPVLT IDGQRYKKVT VNEVTISKSK
260 270 280
YKCRLIKFKD KDYFKILRTK ISYRSRECEG E
Length:281
Mass (Da):31,689
Last modified:June 16, 2009 - v1
Checksum:i829ECA8B8EE1A1AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ53219.1.
RefSeqiWP_003362512.1. NC_012658.1.

Genome annotation databases

EnsemblBacteriaiACQ53219; ACQ53219; CLJ_B2066.
KEGGicbi:CLJ_B2066.
PATRICi19412497. VBICloBot19908_2278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ53219.1.
RefSeqiWP_003362512.1. NC_012658.1.

3D structure databases

ProteinModelPortaliC3KXC0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ53219; ACQ53219; CLJ_B2066.
KEGGicbi:CLJ_B2066.
PATRICi19412497. VBICloBot19908_2278.

Phylogenomic databases

HOGENOMiHOG000227222.
KOiK00858.
OMAiMCHFEIA.
OrthoDBiPOG091H02D1.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADK_CLOB6
AccessioniPrimary (citable) accession number: C3KXC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.