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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Clostridium botulinum (strain 657 / Type Ba4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 738ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBOT515621:GCP3-3798-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:CLJ_B3847
OrganismiClostridium botulinum (strain 657 / Type Ba4)
Taxonomic identifieri515621 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002333: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Formate--tetrahydrofolate ligasePRO_1000215433Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi515621.CLJ_B3847.

Structurei

3D structure databases

ProteinModelPortaliC3KVU4.
SMRiC3KVU4. Positions 5-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3KVU4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKSDIEIAQ ESKMKNIKNI AEKIGLTEED IDLYGKYKCK ISLDVLKSNK
60 70 80 90 100
DKKDGKLILV TAINPTPAGE GKSTVTVGLG QALWKKNKKA VIALREPSLG
110 120 130 140 150
PVFGIKGGAA GGGYSQVVPM EDINLHFTGD MHAITSANNL LAAAIDNHIH
160 170 180 190 200
QGNILKIDQR RILFKRVMDM NDRALRNVIV ALGGKINGFP REDGFMITVA
210 220 230 240 250
SEIMAILCLA EDLMDLKNKM GEILVAYSIE GKPIYCKDLE VQGAMALLMK
260 270 280 290 300
DAIKPNLVQT LENTPAIIHG GPFANIAHGC NSILGTKMAL KLGDYVITEA
310 320 330 340 350
GFGADLGAEK FFDIKCRKAN LKPNCVVIVA TVRALKYNGG ILKENLKEQN
360 370 380 390 400
MEALSKGIEN LGKHIENVNK FGVPAVVAIN KFISDTEEEI EFIKKYCKEL
410 420 430 440 450
GAEVSIAEVW EKGGNGGLEL ADKVLDTIEN KESKFNPIYE ETLNIKQKIE
460 470 480 490 500
TIAQEIYGAE GVDYSKEAEK QISEIEKLDL DKKPVCMAKT QYSLSDDAKL
510 520 530 540 550
LGRPCGFRIN VKEVRISNGA GFIVALTGNV MTMPGLPKKP AANNMNVLSD

GNIVGLF
Length:557
Mass (Da):60,700
Last modified:June 16, 2009 - v1
Checksum:i487147DE422F4B3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ54922.1.
RefSeqiWP_003361711.1. NC_012658.1.
YP_002864537.1. NC_012658.1.

Genome annotation databases

EnsemblBacteriaiACQ54922; ACQ54922; CLJ_B3847.
GeneIDi7880386.
KEGGicbi:CLJ_B3847.
PATRICi19415997. VBICloBot19908_3984.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001083 Genomic DNA. Translation: ACQ54922.1.
RefSeqiWP_003361711.1. NC_012658.1.
YP_002864537.1. NC_012658.1.

3D structure databases

ProteinModelPortaliC3KVU4.
SMRiC3KVU4. Positions 5-555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi515621.CLJ_B3847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ54922; ACQ54922; CLJ_B3847.
GeneIDi7880386.
KEGGicbi:CLJ_B3847.
PATRICi19415997. VBICloBot19908_3984.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCBOT515621:GCP3-3798-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Clostridium botulinum Ba4 strain 657."
    Shrivastava S., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 657 / Type Ba4.

Entry informationi

Entry nameiFTHS_CLOB6
AccessioniPrimary (citable) accession number: C3KVU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: January 7, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.