C3KDZ3 (ALGL_PSEFS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alginate lyase
EC=4.2.2.3

Alternative name(s):
Poly(beta-D-mannuronate) lyase
Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	PFLU_0983

Organism

Pseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP]

Taxonomic identifier

216595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond By similarity. HAMAP MF_00557
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Subcellular location	Periplasm Potential HAMAP MF_00557.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 373	348	Alginate lyase HAMAP MF_00557		PRO_5000460114

Sequences

	Sequence		Length	Mass (Da)
	C3KDZ3 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 77A9C242BD9A8181	FASTA	373	42,323
	10 20 30 40 50 60 MRLPMQKLLI PTLLGLAMFA GSVNAAAPLR PPQGYFAPVE AFKTGDFKND CDAMPPPYTG 70 80 90 100 110 120 SLQFRSKYEG SDKARSTLNV QSEKAFRDST ADITKLEKDT SKRVMQFMRD GRPEQLECTL 130 140 150 160 170 180 NWLTSWAKAD ALMSKDFNHT GKSMRKWALG SMASAYVRLK FSDSHPLANH QQESQLIEAW 190 200 210 220 230 240 FNKLADQVVS DWDNLPLEKT NNHSYWAAWS VMATSVATNR RDLFDWAVKE YKVGVNQVDD 250 260 270 280 290 300 QGFLPNELKR QQRALSYHNY ALPPLSMIAS FALVNGVDLR QENNSALKRL GDKVLAGVKD 310 320 330 340 350 360 PEIFEKKNGK EQDMKDLKED MKYAWLEPFC TLYTCAPDVI ERKHGMQPFK TFRLGGDLTK 370 VYDPTHEKGN KGS

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SBW25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM181176 Genomic DNA. Translation: CAY47248.1.
RefSeq	YP_002870644.1. NC_012660.1.
3D structure databases
ProteinModelPortal	C3KDZ3.
ModBase	Search...
Protein-protein interaction databases
STRING	C3KDZ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7818374.
GenomeReviews	Gene locus PFLU_0983 in contig AM181176_GR.
KEGG	pfs:PFLU0983.
PATRIC	19896198. VBIPseFlu98510_1217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	AAWSVMA.
ProtClustDB	PRK00325.
Enzyme and pathway databases
BioCyc	PFLU216595:PFLU0983-MONOMER.
Family and domain databases
HAMAP	MF_00557. Alginate_lyase. [Tree]
InterPro	IPR022859. Alginate_lyase. IPR008397. Alginate_lyase_dom. IPR008929. Chondroitin_lyas. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
KO	K01729.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
SUPFAM	SSF48230. Chondroitin_lyas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGL_PSEFS

Accession

Primary (citable) accession number: C3KDZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program