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C3KDX4

- SYI_PSEFS

UniProt

C3KDX4 - SYI_PSEFS

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PFLU_0768
Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylate By similarity
Binding sitei611 – 6111ATP By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity
Metal bindingi929 – 9291Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PFLU_0768
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002332: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216244Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi216595.PFLU0768.

Structurei

3D structure databases

ProteinModelPortaliC3KDX4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi608 – 6125"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3KDX4-1 [UniParc]FASTAAdd to Basket

« Hide

MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYGK LREIGKDRPK    50
FVLHDGPPYA NGTIHIGHAL NKILKDMILR SKTLSGFDAP YVPGWDCHGL 100
PIEHKVEVTY GKNLGADKTR ELCRAYATEQ IEGQKSEFIR LGVLGEWDNP 150
YKTMNFKNEA GEIRALAEIV KGGFVFKGLK PVNWCFDCGS ALAEAEVEYE 200
DKKSSTIDVA FPVADDAKLA EAFGLASLSK PAAIVIWTTT PWTIPANQAL 250
NVHPEFTYAL VDVGDRLLVL AEEMVEACLA RYELQGSVIA TTTGSALELI 300
NFRHPFYDRL SPVYLADYVE LGAGTGIVHS APAYGVDDFV TCKAYGMVND 350
DILNPVQSNG VYAPSLEFFG GQFIFKANDS IIDKLSEVGS LLHTETIKHS 400
YMHCWRHKTP LIYRATAQWF IGMDKEPTSG DTLRVRSLKA IEDTQFVPAW 450
GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LNKESGELHP RTVELMEEVA 500
QRVELEGIEA WFKMDAAELL GDEAPQYDKI SDTLDVWFDS GTTHWHVLRG 550
SHPMGHETGP RADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF 600
TVDETGRKMS KSLKNVIEPK KINDTLGADI MRLWVASTDY SGEIAVSDQI 650
LARSADAYRR IRNTARFLLS NLTGFNPATD ILPAEDMLAL DRWAVDRTLL 700
LQRELQENYG EYRFWNVYSK IHNFCVQELG GFYLDIIKDR QYTTGANSKA 750
RRSAQTALYH ISEALVRWIA PILAFTADEL WEYLPGERNE SVMLNTWYEG 800
LTELPADFEL GREYWEGVMA VKVAVNKELE VQRAAKAVGG NLQAEVTLFA 850
EAGLTADLAK LSNELRFVLI TSTASLAPFA QAPADAVATE VPGLKLKVVK 900
SAFPKCARCW HCREDVGVNP EHPEICGRCV DNISGAGEVR HYA 943
Length:943
Mass (Da):105,453
Last modified:June 16, 2009 - v1
Checksum:iAFEDCE736855C2A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM181176 Genomic DNA. Translation: CAY47037.1.
RefSeqiYP_002870433.1. NC_012660.1.

Genome annotation databases

EnsemblBacteriaiCAY47037; CAY47037; PFLU_0768.
GeneIDi7820330.
KEGGipfs:PFLU0768.
PATRICi19895762. VBIPseFlu98510_1004.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM181176 Genomic DNA. Translation: CAY47037.1 .
RefSeqi YP_002870433.1. NC_012660.1.

3D structure databases

ProteinModelPortali C3KDX4.
ModBasei Search...

Protein-protein interaction databases

STRINGi 216595.PFLU0768.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAY47037 ; CAY47037 ; PFLU_0768 .
GeneIDi 7820330.
KEGGi pfs:PFLU0768.
PATRICi 19895762. VBIPseFlu98510_1004.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SBW25.

Entry informationi

Entry nameiSYI_PSEFS
AccessioniPrimary (citable) accession number: C3KDX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi