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Protein

Imidazolonepropionase

Gene

hutI

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Fe2+SAAS annotation
  • Zn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 1 zinc or iron ion per subunit.UniRule annotation

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 3 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (PFLU_0366), Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Zinc or ironUniRule annotation1
Metal bindingi69Zinc or ironUniRule annotation1
Binding sitei76SubstrateUniRule annotation1
Binding sitei89SubstrateUniRule annotation1
Binding sitei139SubstrateUniRule annotation1
Binding sitei172SubstrateUniRule annotation1
Metal bindingi237Zinc or ironUniRule annotation1
Binding sitei240SubstrateUniRule annotation1
Metal bindingi312Zinc or ironUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation
Biological processHistidine metabolismUniRule annotationSAAS annotation
LigandIronUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00379; UER00551

Names & Taxonomyi

Protein namesi
Recommended name:
ImidazolonepropionaseUniRule annotationSAAS annotation (EC:3.5.2.7UniRule annotationSAAS annotation)
Alternative name(s):
Imidazolone-5-propionate hydrolaseUniRule annotation
Gene namesi
Name:hutIUniRule annotation
Ordered Locus Names:PFLU_0369Imported
OrganismiPseudomonas fluorescens (strain SBW25)Imported
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002332 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiC3K811

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU0369

Structurei

3D structure databases

ProteinModelPortaliC3K811
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 375Amidohydro-relInterPro annotationAdd BLAST318

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. HutI family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CI2 Bacteria
COG1228 LUCA
KOiK01468
OMAiCMMRAGT
OrthoDBiPOG091H0EIK

Family and domain databases

CDDicd01296 Imidazolone-5PH, 1 hit
Gene3Di2.30.40.10, 2 hits
HAMAPiMF_00372 HutI, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR005920 HutI
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
PANTHERiPTHR42752 PTHR42752, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR01224 hutI, 1 hit

Sequencei

Sequence statusi: Complete.

C3K811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKTLWQHCH VATMAQGKYS IIEDAALVTA GSLIEWVGPR SQVPTADYAQ
60 70 80 90 100
VHDLHGAWVT PGLIDCHTHT VFGGNRSGEF EQRLEGVSYA EIAAKGGGIA
110 120 130 140 150
STVRATRAAT EDELFASAEK RLRSLLRDGV TTVEIKSGYG LDLVNERKLL
160 170 180 190 200
RVARRLGEAL PVSVRATCLA AHALPPEYKD RADDYIDHIC TEMLPALAAE
210 220 230 240 250
GLVDAVDAFC EYLAFSTEQV ERVFKVAQQL GLPVKLHAEQ LSSLHGSSLA
260 270 280 290 300
ARYHALSADH LEFMTEEDAI AMAASGTVAV LLPGAFYFLR ETQLPPMEAL
310 320 330 340 350
RKHGVKIAIA SDLNPGTSPA LSVRLMLNMA CTLFRMTPEE ALAGATQHAA
360 370 380 390 400
TALGMGDTHG SLEVGKVADF VAWQIDRPAD LAYWLGGELD KRVVRHGVDV

TV
Length:402
Mass (Da):43,437
Last modified:June 16, 2009 - v1
Checksum:i720EC70C8508BC5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA Translation: CAY46646.1

Genome annotation databases

EnsemblBacteriaiCAY46646; CAY46646; PFLU_0369
KEGGipfs:PFLU_0369

Similar proteinsi

Entry informationi

Entry nameiC3K811_PSEFS
AccessioniPrimary (citable) accession number: C3K811
Entry historyiIntegrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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