C3K811 (C3K811_PSEFS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Imidazolonepropionase HAMAP-Rule MF_00372
EC=3.5.2.7 HAMAP-Rule MF_00372

Alternative name(s):
Imidazolone-5-propionate hydrolase HAMAP-Rule MF_00372

Gene names

Name:	hutI HAMAP-Rule MF_00372
Ordered Locus Names:	PFLU_0369 EMBL CAY46646.1

Organism

Pseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP] EMBL CAY46646.1

Taxonomic identifier

216595 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas fluorescens

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP-Rule MF_00372 SAAS SAAS005920
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP-Rule MF_00372 SAAS SAAS005920
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP-Rule MF_00372 SAAS SAAS005920
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00372 SAAS SAAS005920.
Sequence similarities	Belongs to the HutI family. HAMAP-Rule MF_00372

Ontologies

Keywords
Biological process	Histidine metabolism HAMAP-Rule MF_00372 SAAS SAAS005920
Cellular component	Cytoplasm HAMAP-Rule MF_00372 SAAS SAAS005920
Ligand	Iron HAMAP-Rule MF_00372 SAAS SAAS005920 Metal-binding HAMAP-Rule MF_00372 SAAS SAAS005920 Zinc HAMAP-Rule MF_00372 SAAS SAAS005920
Molecular function	Hydrolase HAMAP-Rule MF_00372 SAAS SAAS005920
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: UniProtKB-UniPathway histidine catabolic process to glutamate and formate Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	67	1	Zinc or iron By similarity HAMAP-Rule MF_00372
	Metal binding	69	1	Zinc or iron By similarity HAMAP-Rule MF_00372
	Metal binding	237	1	Zinc or iron By similarity HAMAP-Rule MF_00372
	Metal binding	312	1	Zinc or iron By similarity HAMAP-Rule MF_00372
	Binding site	76	1	Substrate By similarity HAMAP-Rule MF_00372
	Binding site	89	1	Substrate By similarity HAMAP-Rule MF_00372
	Binding site	139	1	Substrate By similarity HAMAP-Rule MF_00372
	Binding site	172	1	Substrate By similarity HAMAP-Rule MF_00372
	Binding site	240	1	Substrate By similarity HAMAP-Rule MF_00372

Sequences

	Sequence		Length	Mass (Da)
	C3K811 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 720EC70C8508BC5C	FASTA	402	43,437
	10 20 30 40 50 60 MMKTLWQHCH VATMAQGKYS IIEDAALVTA GSLIEWVGPR SQVPTADYAQ VHDLHGAWVT 70 80 90 100 110 120 PGLIDCHTHT VFGGNRSGEF EQRLEGVSYA EIAAKGGGIA STVRATRAAT EDELFASAEK 130 140 150 160 170 180 RLRSLLRDGV TTVEIKSGYG LDLVNERKLL RVARRLGEAL PVSVRATCLA AHALPPEYKD 190 200 210 220 230 240 RADDYIDHIC TEMLPALAAE GLVDAVDAFC EYLAFSTEQV ERVFKVAQQL GLPVKLHAEQ 250 260 270 280 290 300 LSSLHGSSLA ARYHALSADH LEFMTEEDAI AMAASGTVAV LLPGAFYFLR ETQLPPMEAL 310 320 330 340 350 360 RKHGVKIAIA SDLNPGTSPA LSVRLMLNMA CTLFRMTPEE ALAGATQHAA TALGMGDTHG 370 380 390 400 SLEVGKVADF VAWQIDRPAD LAYWLGGELD KRVVRHGVDV TV

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.X., Moon C.D., Gehrig S.M., Godfrey S.A., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51-R51(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SBW25 EMBL CAY46646.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM181176 Genomic DNA. Translation: CAY46646.1.
RefSeq	YP_002870049.1. NC_012660.1.
3D structure databases
ProteinModelPortal	C3K811.
ModBase	Search...
Protein-protein interaction databases
STRING	216595.PFLU0369.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAY46646; CAY46646; PFLU_0369.
GeneID	7819195.
KEGG	pfs:PFLU0369.
PATRIC	19894948. VBIPseFlu98510_0604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1228.
KO	K01468.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Enzyme and pathway databases
UniPathway	UPA00379; UER00551.
Family and domain databases
HAMAP	MF_00372. HutI.
InterPro	IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
PANTHER	PTHR22642. PTHR22642. 1 hit.
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C3K811_PSEFS

Accession

Primary (citable) accession number: C3K811

Entry history

Integrated into UniProtKB/TrEMBL:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)