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Protein

Imidazolonepropionase

Gene

hutI

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationSAAS annotation, Fe2+UniRule annotationSAAS annotationNote: Binds 1 zinc or iron ion per subunit.UniRule annotationSAAS annotation

Pathway:iL-histidine degradation into L-glutamate

This protein is involved in step 3 and 3 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (PFLU_0366), Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI), Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Pathway:iL-histidine degradation into L-glutamate

This protein is involved in step 3 and 3 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (PFLU_0366), Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI), Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Zinc or ironUniRule annotation
Metal bindingi69 – 691Zinc or ironUniRule annotation
Binding sitei76 – 761SubstrateUniRule annotation
Binding sitei89 – 891SubstrateUniRule annotation
Binding sitei139 – 1391SubstrateUniRule annotation
Binding sitei172 – 1721SubstrateUniRule annotation
Metal bindingi237 – 2371Zinc or ironUniRule annotation
Binding sitei240 – 2401SubstrateUniRule annotation
Metal bindingi312 – 3121Zinc or ironUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation

Keywords - Biological processi

Histidine metabolismUniRule annotationSAAS annotation

Keywords - Ligandi

IronUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00379; UER00551.
UPA00379; UER00551.

Names & Taxonomyi

Protein namesi
Recommended name:
ImidazolonepropionaseUniRule annotationSAAS annotation (EC:3.5.2.7UniRule annotationSAAS annotation)
Alternative name(s):
Imidazolone-5-propionate hydrolaseUniRule annotation
Gene namesi
Name:hutIUniRule annotation
Ordered Locus Names:PFLU_0369Imported
OrganismiPseudomonas fluorescens (strain SBW25)Imported
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002332 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU0369.

Structurei

3D structure databases

ProteinModelPortaliC3K811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HutI family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1228.
KOiK01468.
OMAiIATDCNP.
OrthoDBiEOG63C0XD.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_00372. HutI.
InterProiIPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
TIGRFAMsiTIGR01224. hutI. 1 hit.

Sequencei

Sequence statusi: Complete.

C3K811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKTLWQHCH VATMAQGKYS IIEDAALVTA GSLIEWVGPR SQVPTADYAQ
60 70 80 90 100
VHDLHGAWVT PGLIDCHTHT VFGGNRSGEF EQRLEGVSYA EIAAKGGGIA
110 120 130 140 150
STVRATRAAT EDELFASAEK RLRSLLRDGV TTVEIKSGYG LDLVNERKLL
160 170 180 190 200
RVARRLGEAL PVSVRATCLA AHALPPEYKD RADDYIDHIC TEMLPALAAE
210 220 230 240 250
GLVDAVDAFC EYLAFSTEQV ERVFKVAQQL GLPVKLHAEQ LSSLHGSSLA
260 270 280 290 300
ARYHALSADH LEFMTEEDAI AMAASGTVAV LLPGAFYFLR ETQLPPMEAL
310 320 330 340 350
RKHGVKIAIA SDLNPGTSPA LSVRLMLNMA CTLFRMTPEE ALAGATQHAA
360 370 380 390 400
TALGMGDTHG SLEVGKVADF VAWQIDRPAD LAYWLGGELD KRVVRHGVDV

TV
Length:402
Mass (Da):43,437
Last modified:June 16, 2009 - v1
Checksum:i720EC70C8508BC5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA. Translation: CAY46646.1.

Genome annotation databases

EnsemblBacteriaiCAY46646; CAY46646; PFLU_0369.
KEGGipfs:PFLU0369.
PATRICi19894948. VBIPseFlu98510_0604.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA. Translation: CAY46646.1.

3D structure databases

ProteinModelPortaliC3K811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216595.PFLU0369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAY46646; CAY46646; PFLU_0369.
KEGGipfs:PFLU0369.
PATRICi19894948. VBIPseFlu98510_0604.

Phylogenomic databases

eggNOGiCOG1228.
KOiK01468.
OMAiIATDCNP.
OrthoDBiEOG63C0XD.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00551.
UPA00379; UER00551.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_00372. HutI.
InterProiIPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
TIGRFAMsiTIGR01224. hutI. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SBW25Imported.

Entry informationi

Entry nameiC3K811_PSEFS
AccessioniPrimary (citable) accession number: C3K811
Entry historyi
Integrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: July 22, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.