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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

BioCyciPFLU216595:G1G1K-915-MONOMER
UniPathwayiUPA00074; UER00133
UPA00074; UER00135

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:PFLU_0612
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002332 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002032531 – 535Bifunctional purine biosynthesis protein PurHAdd BLAST535

Proteomic databases

PRIDEiC3K6E0

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU0612

Structurei

3D structure databases

ProteinModelPortaliC3K6E0
SMRiC3K6E0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 151MGS-likePROSITE-ProRule annotationAdd BLAST146

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DC1 Bacteria
COG0138 LUCA
KOiK00602
OMAiDLLFAWK
OrthoDBiPOG091H00UT

Family and domain databases

Gene3Di3.40.140.20, 2 hits
3.40.50.1380, 1 hit
HAMAPiMF_00139 PurH, 1 hit
InterProiView protein in InterPro
IPR024051 AICAR_Tfase_dup_dom_sf
IPR016193 Cytidine_deaminase-like
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR002695 PurH-like
PANTHERiPTHR11692 PTHR11692, 1 hit
PfamiView protein in Pfam
PF01808 AICARFT_IMPCHas, 1 hit
PF02142 MGS, 1 hit
PIRSFiPIRSF000414 AICARFT_IMPCHas, 1 hit
SMARTiView protein in SMART
SM00798 AICARFT_IMPCHas, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF52335 SSF52335, 1 hit
SSF53927 SSF53927, 1 hit
TIGRFAMsiTIGR00355 purH, 1 hit
PROSITEiView protein in PROSITE
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

C3K6E0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQTTRLPI RRALISVSDK TGILEFAREL EALGVEILST GGTFKLLQDN
60 70 80 90 100
GVAAVEVADY TGFAEMMDGR VKTLHPKIHG GILGRRGIDD AIMTEHGIKP
110 120 130 140 150
IDLVAVNLYP FEATINKPGC DLPTAIENID IGGPTMVRSA AKNHKDVAIV
160 170 180 190 200
VNASDYAQVL ESLKAGGLTY AQRFDLMLKA FEHTAAYDGM IANYMGTVNQ
210 220 230 240 250
AAETLSTEGR SQFPRTFNSQ FIKAQEMRYG ENPHQSAAFY VEAKPAEVGI
260 270 280 290 300
ATATQLQGKE LSYNNVADTD AALECVKSFV KPACVIVKHA NPCGVAVSPD
310 320 330 340 350
AEGGIRQAYE LAYATDTESA FGGIIAFNRE LDAETAKAIV ERQFVEVIIA
360 370 380 390 400
PSVSEEARAI VAAKANVRLL ACGEWSADRA AAWDYKRVNG GLLVQSRDIG
410 420 430 440 450
MIGSEDLKVV TKRAPTEQEI NDLIFAWKVA KYVKSNAIVY AKNRQTIGVG
460 470 480 490 500
AGQMSRVNSA RIAAIKAEHA GLQVVGSVMA SDAFFPFRDG LDNAAKAGVT
510 520 530
AVIQPGGSMR DAEVIAAADE AGIAMVFTGM RHFRH
Length:535
Mass (Da):57,406
Last modified:June 16, 2009 - v1
Checksum:iEE45FAEA99852CE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA Translation: CAY46881.1
RefSeqiWP_012721993.1, NC_012660.1

Genome annotation databases

EnsemblBacteriaiCAY46881; CAY46881; PFLU_0612
KEGGipfs:PFLU_0612
PATRICifig|216595.4.peg.847

Similar proteinsi

Entry informationi

Entry nameiPUR9_PSEFS
AccessioniPrimary (citable) accession number: C3K6E0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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