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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: UniProtKB-HAMAP
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:PFLU_0612
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002332: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Bifunctional purine biosynthesis protein PurHPRO_1000203253Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU0612.

Structurei

3D structure databases

ProteinModelPortaliC3K6E0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0138.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

C3K6E0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDQTTRLPI RRALISVSDK TGILEFAREL EALGVEILST GGTFKLLQDN
60 70 80 90 100
GVAAVEVADY TGFAEMMDGR VKTLHPKIHG GILGRRGIDD AIMTEHGIKP
110 120 130 140 150
IDLVAVNLYP FEATINKPGC DLPTAIENID IGGPTMVRSA AKNHKDVAIV
160 170 180 190 200
VNASDYAQVL ESLKAGGLTY AQRFDLMLKA FEHTAAYDGM IANYMGTVNQ
210 220 230 240 250
AAETLSTEGR SQFPRTFNSQ FIKAQEMRYG ENPHQSAAFY VEAKPAEVGI
260 270 280 290 300
ATATQLQGKE LSYNNVADTD AALECVKSFV KPACVIVKHA NPCGVAVSPD
310 320 330 340 350
AEGGIRQAYE LAYATDTESA FGGIIAFNRE LDAETAKAIV ERQFVEVIIA
360 370 380 390 400
PSVSEEARAI VAAKANVRLL ACGEWSADRA AAWDYKRVNG GLLVQSRDIG
410 420 430 440 450
MIGSEDLKVV TKRAPTEQEI NDLIFAWKVA KYVKSNAIVY AKNRQTIGVG
460 470 480 490 500
AGQMSRVNSA RIAAIKAEHA GLQVVGSVMA SDAFFPFRDG LDNAAKAGVT
510 520 530
AVIQPGGSMR DAEVIAAADE AGIAMVFTGM RHFRH
Length:535
Mass (Da):57,406
Last modified:June 16, 2009 - v1
Checksum:iEE45FAEA99852CE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA. Translation: CAY46881.1.
RefSeqiWP_012721993.1. NC_012660.1.
YP_002870280.1. NC_012660.1.

Genome annotation databases

EnsemblBacteriaiCAY46881; CAY46881; PFLU_0612.
GeneIDi7819054.
KEGGipfs:PFLU0612.
PATRICi19895438. VBIPseFlu98510_0847.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA. Translation: CAY46881.1.
RefSeqiWP_012721993.1. NC_012660.1.
YP_002870280.1. NC_012660.1.

3D structure databases

ProteinModelPortaliC3K6E0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216595.PFLU0612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAY46881; CAY46881; PFLU_0612.
GeneIDi7819054.
KEGGipfs:PFLU0612.
PATRICi19895438. VBIPseFlu98510_0847.

Phylogenomic databases

eggNOGiCOG0138.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SBW25.

Entry informationi

Entry nameiPUR9_PSEFS
AccessioniPrimary (citable) accession number: C3K6E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: February 4, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.