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C3K5E4 (GLND_PSEFS) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme

Short name=UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein

Including the following 2 domains:

  1. [Protein-PII] uridylyltransferase
    Short name=PII uridylyltransferase
    Short name=UTase
    EC=2.7.7.59
  2. [Protein-PII]-UMP uridylyl-removing enzyme
    Short name=UR
    EC=3.1.4.-
Gene names
Name:glnD
Ordered Locus Names:PFLU_1268
OrganismPseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP]
Taxonomic identifier216595 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length900 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity. HAMAP-Rule MF_00277

Catalytic activity

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277

Uridylyl-[protein-PII] + H2O = UMP + [protein-PII]. HAMAP-Rule MF_00277

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00277

Enzyme regulation

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity. HAMAP-Rule MF_00277

Domain

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity. HAMAP-Rule MF_00277

Sequence similarities

Belongs to the GlnD family.

Contains 2 ACT domains.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzyme HAMAP-Rule MF_00277
PRO_1000204800

Regions

Domain484 – 56986HD
Domain706 – 78479ACT 1
Domain816 – 89277ACT 2
Region1 – 342342Uridylyltransferase HAMAP-Rule MF_00277
Region343 – 705363Uridylyl-removing HAMAP-Rule MF_00277

Sequences

Sequence LengthMass (Da)Tools
C3K5E4 [UniParc].

Last modified June 16, 2009. Version 1.
Checksum: 539B823093DD9705

FASTA900103,012
        10         20         30         40         50         60 
MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAREVLD GRFRSGRDIR RLIEDRAWFV 

        70         80         90        100        110        120 
DNILQKAWEQ FSWSEDADIA LVAVGGYGRG ELHPYSDIDL LILLDSADHE IFRDSIERFL 

       130        140        150        160        170        180 
TLLWDIGLEV GQSVRSVDEC AEEARADLTV ITNLMESRTI AGPERLRQRM LEVTSTAHMW 

       190        200        210        220        230        240 
PSKDFFLAKR AEQKARHHKY NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLRAL 

       250        260        270        280        290        300 
AGEGFLVESE NALLASSQEF LWKVRYALHM LAGRSEDRLL FDHQRSIATL LGFEGEDAKT 

       310        320        330        340        350        360 
SIESFMQQYY RVVMSIAQLS DLIIQHFEEV ILAPEDEAPP QPINSRFQLH DGYIEARNDN 

       370        380        390        400        410        420 
VFRRTPFAML EIFVLMAQQP EIKGVRADTI RLLRENRHLI DDDFRNDIRN TSLFIELFKC 

       430        440        450        460        470        480 
KIGIHRNLRR MNRYGILGRY LPEFGFIVGQ MQHDLFHIYT VDAHTLNLIK HLRKLQYTQV 

       490        500        510        520        530        540 
SEKFPLASKL MAKLPKPELI YLAGLYHDIG KGRHGDHSEI GAVDAEAFCQ RHQLPLWDSR 

       550        560        570        580        590        600 
LIVWLVQNHL VMSTTAQRKD LSDPQVIHDF AGIVEDETRL DYLYVLTVSD INATNPTLWN 

       610        620        630        640        650        660 
SWRASLLRQL YTETKRALRR GLENPVDREE QIRRTQSAAL DILVRGGNDP DDVEQLWSQL 

       670        680        690        700        710        720 
GDDYFLRHTA GDVAWHSDAI LQQPADGGPL VLIKETTQRE FEGGTQIFIY APDQHDFFAV 

       730        740        750        760        770        780 
TVAAMDQLNL NIHDARVITS SSQFTLDTYI VLDTEGESIG DNPVRVKKIR EGLTEALRNP 

       790        800        810        820        830        840 
DDYPTIIQRR VPRQLKHFAF APQVTISNDA QRPVTVLELS APDRPGLLAR IGGIFLEFDL 

       850        860        870        880        890        900 
SLQNAKIATL GERVEDVFFI TDADNQPLSD PELCRRLQDA IVQQLSVTQE PGVELTRLTI 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM181176 Genomic DNA. Translation: CAY47525.1.
RefSeqYP_002870920.1. NC_012660.1.

3D structure databases

ProteinModelPortalC3K5E4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216595.PFLU1268.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAY47525; CAY47525; PFLU_1268.
GeneID7820447.
KEGGpfs:PFLU1268.
PATRIC19896762. VBIPseFlu98510_1499.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2844.
KOK00990.
OMAHHLLMSV.
OrthoDBEOG6CCH44.

Family and domain databases

Gene3D1.10.3210.10. 1 hit.
HAMAPMF_00277. PII_uridylyl_transf.
InterProIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFPIRSF006288. PII_uridyltransf. 1 hit.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsTIGR01693. UTase_glnD. 1 hit.
PROSITEPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLND_PSEFS
AccessionPrimary (citable) accession number: C3K5E4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families