Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:PFLU_1268
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002048001 – 900Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST900

Proteomic databases

PRIDEiC3K5E4

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU1268

Structurei

3D structure databases

ProteinModelPortaliC3K5E4
SMRiC3K5E4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini461 – 583HDPROSITE-ProRule annotationAdd BLAST123
Domaini706 – 784ACT 1UniRule annotationAdd BLAST79
Domaini816 – 892ACT 2UniRule annotationAdd BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 342UridylyltransferaseAdd BLAST342
Regioni343 – 705Uridylyl-removingAdd BLAST363

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA
KOiK00990
OMAiARICGYF
OrthoDBiPOG091H04HO

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 2 hits
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

C3K5E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAREVLD GRFRSGRDIR
60 70 80 90 100
RLIEDRAWFV DNILQKAWEQ FSWSEDADIA LVAVGGYGRG ELHPYSDIDL
110 120 130 140 150
LILLDSADHE IFRDSIERFL TLLWDIGLEV GQSVRSVDEC AEEARADLTV
160 170 180 190 200
ITNLMESRTI AGPERLRQRM LEVTSTAHMW PSKDFFLAKR AEQKARHHKY
210 220 230 240 250
NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLRAL AGEGFLVESE
260 270 280 290 300
NALLASSQEF LWKVRYALHM LAGRSEDRLL FDHQRSIATL LGFEGEDAKT
310 320 330 340 350
SIESFMQQYY RVVMSIAQLS DLIIQHFEEV ILAPEDEAPP QPINSRFQLH
360 370 380 390 400
DGYIEARNDN VFRRTPFAML EIFVLMAQQP EIKGVRADTI RLLRENRHLI
410 420 430 440 450
DDDFRNDIRN TSLFIELFKC KIGIHRNLRR MNRYGILGRY LPEFGFIVGQ
460 470 480 490 500
MQHDLFHIYT VDAHTLNLIK HLRKLQYTQV SEKFPLASKL MAKLPKPELI
510 520 530 540 550
YLAGLYHDIG KGRHGDHSEI GAVDAEAFCQ RHQLPLWDSR LIVWLVQNHL
560 570 580 590 600
VMSTTAQRKD LSDPQVIHDF AGIVEDETRL DYLYVLTVSD INATNPTLWN
610 620 630 640 650
SWRASLLRQL YTETKRALRR GLENPVDREE QIRRTQSAAL DILVRGGNDP
660 670 680 690 700
DDVEQLWSQL GDDYFLRHTA GDVAWHSDAI LQQPADGGPL VLIKETTQRE
710 720 730 740 750
FEGGTQIFIY APDQHDFFAV TVAAMDQLNL NIHDARVITS SSQFTLDTYI
760 770 780 790 800
VLDTEGESIG DNPVRVKKIR EGLTEALRNP DDYPTIIQRR VPRQLKHFAF
810 820 830 840 850
APQVTISNDA QRPVTVLELS APDRPGLLAR IGGIFLEFDL SLQNAKIATL
860 870 880 890 900
GERVEDVFFI TDADNQPLSD PELCRRLQDA IVQQLSVTQE PGVELTRLTI
Length:900
Mass (Da):103,012
Last modified:June 16, 2009 - v1
Checksum:i539B823093DD9705
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA Translation: CAY47525.1
RefSeqiWP_012722593.1, NC_012660.1

Genome annotation databases

EnsemblBacteriaiCAY47525; CAY47525; PFLU_1268
KEGGipfs:PFLU_1268

Entry informationi

Entry nameiGLND_PSEFS
AccessioniPrimary (citable) accession number: C3K5E4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health