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C3K5E4

- GLND_PSEFS

UniProt

C3K5E4 - GLND_PSEFS

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:PFLU_1268
    OrganismiPseudomonas fluorescens (strain SBW25)
    Taxonomic identifieri216595 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002332: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000204800Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi216595.PFLU1268.

    Structurei

    3D structure databases

    ProteinModelPortaliC3K5E4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini484 – 56986HDUniRule annotationAdd
    BLAST
    Domaini706 – 78479ACT 1UniRule annotationAdd
    BLAST
    Domaini816 – 89277ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 342342UridylyltransferaseAdd
    BLAST
    Regioni343 – 705363Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C3K5E4-1 [UniParc]FASTAAdd to Basket

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    MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAREVLD GRFRSGRDIR    50
    RLIEDRAWFV DNILQKAWEQ FSWSEDADIA LVAVGGYGRG ELHPYSDIDL 100
    LILLDSADHE IFRDSIERFL TLLWDIGLEV GQSVRSVDEC AEEARADLTV 150
    ITNLMESRTI AGPERLRQRM LEVTSTAHMW PSKDFFLAKR AEQKARHHKY 200
    NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLRAL AGEGFLVESE 250
    NALLASSQEF LWKVRYALHM LAGRSEDRLL FDHQRSIATL LGFEGEDAKT 300
    SIESFMQQYY RVVMSIAQLS DLIIQHFEEV ILAPEDEAPP QPINSRFQLH 350
    DGYIEARNDN VFRRTPFAML EIFVLMAQQP EIKGVRADTI RLLRENRHLI 400
    DDDFRNDIRN TSLFIELFKC KIGIHRNLRR MNRYGILGRY LPEFGFIVGQ 450
    MQHDLFHIYT VDAHTLNLIK HLRKLQYTQV SEKFPLASKL MAKLPKPELI 500
    YLAGLYHDIG KGRHGDHSEI GAVDAEAFCQ RHQLPLWDSR LIVWLVQNHL 550
    VMSTTAQRKD LSDPQVIHDF AGIVEDETRL DYLYVLTVSD INATNPTLWN 600
    SWRASLLRQL YTETKRALRR GLENPVDREE QIRRTQSAAL DILVRGGNDP 650
    DDVEQLWSQL GDDYFLRHTA GDVAWHSDAI LQQPADGGPL VLIKETTQRE 700
    FEGGTQIFIY APDQHDFFAV TVAAMDQLNL NIHDARVITS SSQFTLDTYI 750
    VLDTEGESIG DNPVRVKKIR EGLTEALRNP DDYPTIIQRR VPRQLKHFAF 800
    APQVTISNDA QRPVTVLELS APDRPGLLAR IGGIFLEFDL SLQNAKIATL 850
    GERVEDVFFI TDADNQPLSD PELCRRLQDA IVQQLSVTQE PGVELTRLTI 900
    Length:900
    Mass (Da):103,012
    Last modified:June 16, 2009 - v1
    Checksum:i539B823093DD9705
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM181176 Genomic DNA. Translation: CAY47525.1.
    RefSeqiYP_002870920.1. NC_012660.1.

    Genome annotation databases

    EnsemblBacteriaiCAY47525; CAY47525; PFLU_1268.
    GeneIDi7820447.
    KEGGipfs:PFLU1268.
    PATRICi19896762. VBIPseFlu98510_1499.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM181176 Genomic DNA. Translation: CAY47525.1 .
    RefSeqi YP_002870920.1. NC_012660.1.

    3D structure databases

    ProteinModelPortali C3K5E4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216595.PFLU1268.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAY47525 ; CAY47525 ; PFLU_1268 .
    GeneIDi 7820447.
    KEGGi pfs:PFLU1268.
    PATRICi 19896762. VBIPseFlu98510_1499.

    Phylogenomic databases

    eggNOGi COG2844.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SBW25.

    Entry informationi

    Entry nameiGLND_PSEFS
    AccessioniPrimary (citable) accession number: C3K5E4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3