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C3K5E4

- GLND_PSEFS

UniProt

C3K5E4 - GLND_PSEFS

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, PFLU_1268
Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PFLU_1268
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002332: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000204800Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU1268.

Structurei

3D structure databases

ProteinModelPortaliC3K5E4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini484 – 56986HD
Add
BLAST
Domaini706 – 78479ACT 1
Add
BLAST
Domaini816 – 89277ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotation
Add
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C3K5E4-1 [UniParc]FASTAAdd to Basket

« Hide

MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAREVLD GRFRSGRDIR    50
RLIEDRAWFV DNILQKAWEQ FSWSEDADIA LVAVGGYGRG ELHPYSDIDL 100
LILLDSADHE IFRDSIERFL TLLWDIGLEV GQSVRSVDEC AEEARADLTV 150
ITNLMESRTI AGPERLRQRM LEVTSTAHMW PSKDFFLAKR AEQKARHHKY 200
NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLRAL AGEGFLVESE 250
NALLASSQEF LWKVRYALHM LAGRSEDRLL FDHQRSIATL LGFEGEDAKT 300
SIESFMQQYY RVVMSIAQLS DLIIQHFEEV ILAPEDEAPP QPINSRFQLH 350
DGYIEARNDN VFRRTPFAML EIFVLMAQQP EIKGVRADTI RLLRENRHLI 400
DDDFRNDIRN TSLFIELFKC KIGIHRNLRR MNRYGILGRY LPEFGFIVGQ 450
MQHDLFHIYT VDAHTLNLIK HLRKLQYTQV SEKFPLASKL MAKLPKPELI 500
YLAGLYHDIG KGRHGDHSEI GAVDAEAFCQ RHQLPLWDSR LIVWLVQNHL 550
VMSTTAQRKD LSDPQVIHDF AGIVEDETRL DYLYVLTVSD INATNPTLWN 600
SWRASLLRQL YTETKRALRR GLENPVDREE QIRRTQSAAL DILVRGGNDP 650
DDVEQLWSQL GDDYFLRHTA GDVAWHSDAI LQQPADGGPL VLIKETTQRE 700
FEGGTQIFIY APDQHDFFAV TVAAMDQLNL NIHDARVITS SSQFTLDTYI 750
VLDTEGESIG DNPVRVKKIR EGLTEALRNP DDYPTIIQRR VPRQLKHFAF 800
APQVTISNDA QRPVTVLELS APDRPGLLAR IGGIFLEFDL SLQNAKIATL 850
GERVEDVFFI TDADNQPLSD PELCRRLQDA IVQQLSVTQE PGVELTRLTI 900
Length:900
Mass (Da):103,012
Last modified:June 16, 2009 - v1
Checksum:i539B823093DD9705
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM181176 Genomic DNA. Translation: CAY47525.1.
RefSeqiYP_002870920.1. NC_012660.1.

Genome annotation databases

EnsemblBacteriaiCAY47525; CAY47525; PFLU_1268.
GeneIDi7820447.
KEGGipfs:PFLU1268.
PATRICi19896762. VBIPseFlu98510_1499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM181176 Genomic DNA. Translation: CAY47525.1 .
RefSeqi YP_002870920.1. NC_012660.1.

3D structure databases

ProteinModelPortali C3K5E4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216595.PFLU1268.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAY47525 ; CAY47525 ; PFLU_1268 .
GeneIDi 7820447.
KEGGi pfs:PFLU1268.
PATRICi 19896762. VBIPseFlu98510_1499.

Phylogenomic databases

eggNOGi COG2844.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SBW25.

Entry informationi

Entry nameiGLND_PSEFS
AccessioniPrimary (citable) accession number: C3K5E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi