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Protein

Thymidylate synthase

Gene

thyA

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei159NucleophileUniRule annotation1
Binding sitei1825,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei190dUMPUniRule annotation1
Binding sitei2765,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 127dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi179 – 182dUMPUniRule annotation4
Nucleotide bindingi220 – 222dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:PFLU_5815
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002332 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002017241 – 277Thymidylate synthaseAdd BLAST277

Proteomic databases

PRIDEiC3K3Q8

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi216595.PFLU5815

Structurei

3D structure databases

SMRiC3K3Q8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207 LUCA
KOiK00560
OMAiKQYLDLC
OrthoDBiPOG091H02L6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 2 hits

Sequencei

Sequence statusi: Complete.

C3K3Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLELLND VVTNGLTKGD RTGTGTKAVF ARQYRHNLAD GFPLLTTKKL
60 70 80 90 100
HFKSIANELI WMLSGNTNIK WLNENGVKIW DEWATEDGDL GPVYGEQWTA
110 120 130 140 150
WPTKDGGTIN QIDYMVHTLK TNPNSRRILF HGWNVEYLPD ETKSPQENAR
160 170 180 190 200
NGKQALPPCH LLYQAFVHDG HLSMQLYIRS SDVFLGLPYN TAALALLTHM
210 220 230 240 250
LAQQCDLIPH EIIVTTGDTH AYSNHMEQIR TQLARTPKKL PELVIKRKPA
260 270
SIYDYKFEDF EIVGYDADPS IKADVAI
Length:277
Mass (Da):31,510
Last modified:June 16, 2009 - v1
Checksum:i015A92E606ABC8FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA Translation: CAY53232.1
RefSeqiWP_015886389.1, NC_012660.1

Genome annotation databases

EnsemblBacteriaiCAY53232; CAY53232; PFLU_5815
KEGGipfs:PFLU_5815

Similar proteinsi

Entry informationi

Entry nameiTYSY_PSEFS
AccessioniPrimary (citable) accession number: C3K3Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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