C3K3L6 (TRMB_PSEFS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	PFLU_5772

Organism

Pseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP]

Taxonomic identifier

216595 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas fluorescens

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_1000213442
Regions
	Region	219 – 222	4	Substrate binding Potential
Sites
	Binding site	71	1	S-adenosyl-L-methionine By similarity
	Binding site	96	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity
	Binding site	146	1	S-adenosyl-L-methionine By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	182	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	C3K3L6 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 1AEDE04DFA5CC234	FASTA	241	27,007
	10 20 30 40 50 60 MTESNETPNT PEAGDESKHR RIKSFVMRAG RMTEGQQKGL EQGTPLFVLP LADAPVDYDQ 70 80 90 100 110 120 VFGRSAPRSL EIGFGMGHSL LEMAAAAPDQ DFIGVEVHRP GVGALLNGVL TQGLTNLRVY 130 140 150 160 170 180 DCDAIEVLNR CIADNSLDRL MLFFPDPWHK ARHHKRRIVQ ASFAELVRSK LKVGGILHMA 190 200 210 220 230 240 TDWEPYAEYM LEVMNVAPGY RNLAEDGKCV PRPAERPITK FERRGERLGH GVWDLKFEKL A

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SBW25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM181176 Genomic DNA. Translation: CAY53159.1.
RefSeq	YP_002875262.1. NC_012660.1.
3D structure databases
ProteinModelPortal	C3K3L6.
ModBase	Search...
Protein-protein interaction databases
STRING	216595.PFLU5772.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAY53159; CAY53159; PFLU_5772.
GeneID	7821573.
KEGG	pfs:PFLU5772.
PATRIC	19905664. VBIPseFlu98510_5894.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
OMA	FIHCATD.
ProtClustDB	PRK00121.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_PSEFS

Accession

Primary (citable) accession number: C3K3L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program