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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71S-adenosyl-L-methionineUniRule annotation1
Binding sitei96S-adenosyl-L-methionineUniRule annotation1
Binding sitei123S-adenosyl-L-methionineUniRule annotation1
Active sitei146By similarity1
Binding sitei146S-adenosyl-L-methionineUniRule annotation1
Binding sitei150SubstrateUniRule annotation1
Binding sitei182SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:PFLU_5772
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002332 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002134421 – 241tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST241

Proteomic databases

PRIDEiC3K3L6.

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU5772.

Structurei

3D structure databases

ProteinModelPortaliC3K3L6.
SMRiC3K3L6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 222Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
KOiK03439.
OMAiPDPWHKS.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

C3K3L6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESNETPNT PEAGDESKHR RIKSFVMRAG RMTEGQQKGL EQGTPLFVLP
60 70 80 90 100
LADAPVDYDQ VFGRSAPRSL EIGFGMGHSL LEMAAAAPDQ DFIGVEVHRP
110 120 130 140 150
GVGALLNGVL TQGLTNLRVY DCDAIEVLNR CIADNSLDRL MLFFPDPWHK
160 170 180 190 200
ARHHKRRIVQ ASFAELVRSK LKVGGILHMA TDWEPYAEYM LEVMNVAPGY
210 220 230 240
RNLAEDGKCV PRPAERPITK FERRGERLGH GVWDLKFEKL A
Length:241
Mass (Da):27,007
Last modified:June 16, 2009 - v1
Checksum:i1AEDE04DFA5CC234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA. Translation: CAY53159.1.
RefSeqiWP_015886352.1. NC_012660.1.

Genome annotation databases

EnsemblBacteriaiCAY53159; CAY53159; PFLU_5772.
KEGGipfs:PFLU_5772.

Similar proteinsi

Entry informationi

Entry nameiTRMB_PSEFS
AccessioniPrimary (citable) accession number: C3K3L6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: November 22, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families