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C3K2L8

- LIPA_PSEFS

UniProt

C3K2L8 - LIPA_PSEFS

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Protein
Lipoyl synthase
Gene
lipA, PFLU_5416
Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi96 – 961Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi111 – 1111Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi115 – 1151Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi118 – 1181Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:PFLU_5416
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002332: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Lipoyl synthaseUniRule annotation
PRO_1000204154Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216595.PFLU5416.

Structurei

3D structure databases

ProteinModelPortaliC3K2L8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

C3K2L8-1 [UniParc]FASTAAdd to Basket

« Hide

MTTDAVQTMI PTLDITERPA PAPRAKVEAG VKLRGAEKVA RIPVKIIPTT    50
ELPKKPDWIR VRIPVSPEVD RIKALLRKHK LHSVCEEASC PNLGECFSGG 100
TATFMIMGDI CTRRCPFCDV GHGRPKPLDV NEPESLAIAI ADLKLKYVVI 150
TSVDRDDLRD GGAQHFADCI REIRKLSPNV MLETLVPDYR GRMDVALEIT 200
AAEPPDVFNH NLETVPRLYK AARPGSDYQW SLTLLQKFKQ MMPHIPTKSG 250
LMLGLGETDE EVIEVMKRMR EHDIDMLTLG QYLQPSRSHL PVQRFVHPDT 300
FAWFAEEGYK MGFKNVASGP LVRSSYHADE QAKLVKASLV S 341
Length:341
Mass (Da):38,187
Last modified:June 16, 2009 - v1
Checksum:i34DACB59C29BED50
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM181176 Genomic DNA. Translation: CAY52590.1.
RefSeqiYP_002874914.1. NC_012660.1.

Genome annotation databases

EnsemblBacteriaiCAY52590; CAY52590; PFLU_5416.
GeneIDi7817539.
KEGGipfs:PFLU5416.
PATRICi19904934. VBIPseFlu98510_5539.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM181176 Genomic DNA. Translation: CAY52590.1 .
RefSeqi YP_002874914.1. NC_012660.1.

3D structure databases

ProteinModelPortali C3K2L8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216595.PFLU5416.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAY52590 ; CAY52590 ; PFLU_5416 .
GeneIDi 7817539.
KEGGi pfs:PFLU5416.
PATRICi 19904934. VBIPseFlu98510_5539.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SBW25.

Entry informationi

Entry nameiLIPA_PSEFS
AccessioniPrimary (citable) accession number: C3K2L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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