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C3K2L8

- LIPA_PSEFS

UniProt

C3K2L8 - LIPA_PSEFS

Protein

Lipoyl synthase

Gene

lipA

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi96 – 961Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi111 – 1111Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi115 – 1151Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi118 – 1181Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:PFLU_5416
    OrganismiPseudomonas fluorescens (strain SBW25)
    Taxonomic identifieri216595 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002332: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341Lipoyl synthasePRO_1000204154Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi216595.PFLU5416.

    Structurei

    3D structure databases

    ProteinModelPortaliC3K2L8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C3K2L8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTDAVQTMI PTLDITERPA PAPRAKVEAG VKLRGAEKVA RIPVKIIPTT    50
    ELPKKPDWIR VRIPVSPEVD RIKALLRKHK LHSVCEEASC PNLGECFSGG 100
    TATFMIMGDI CTRRCPFCDV GHGRPKPLDV NEPESLAIAI ADLKLKYVVI 150
    TSVDRDDLRD GGAQHFADCI REIRKLSPNV MLETLVPDYR GRMDVALEIT 200
    AAEPPDVFNH NLETVPRLYK AARPGSDYQW SLTLLQKFKQ MMPHIPTKSG 250
    LMLGLGETDE EVIEVMKRMR EHDIDMLTLG QYLQPSRSHL PVQRFVHPDT 300
    FAWFAEEGYK MGFKNVASGP LVRSSYHADE QAKLVKASLV S 341
    Length:341
    Mass (Da):38,187
    Last modified:June 16, 2009 - v1
    Checksum:i34DACB59C29BED50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM181176 Genomic DNA. Translation: CAY52590.1.
    RefSeqiYP_002874914.1. NC_012660.1.

    Genome annotation databases

    EnsemblBacteriaiCAY52590; CAY52590; PFLU_5416.
    GeneIDi7817539.
    KEGGipfs:PFLU5416.
    PATRICi19904934. VBIPseFlu98510_5539.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM181176 Genomic DNA. Translation: CAY52590.1 .
    RefSeqi YP_002874914.1. NC_012660.1.

    3D structure databases

    ProteinModelPortali C3K2L8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216595.PFLU5416.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAY52590 ; CAY52590 ; PFLU_5416 .
    GeneIDi 7817539.
    KEGGi pfs:PFLU5416.
    PATRICi 19904934. VBIPseFlu98510_5539.

    Phylogenomic databases

    eggNOGi COG0320.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SBW25.

    Entry informationi

    Entry nameiLIPA_PSEFS
    AccessioniPrimary (citable) accession number: C3K2L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3