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C3K2L2

- SYL_PSEFS

UniProt

C3K2L2 - SYL_PSEFS

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei630 – 6301ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:PFLU_5410
    OrganismiPseudomonas fluorescens (strain SBW25)
    Taxonomic identifieri216595 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002332: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 868868Leucine--tRNA ligasePRO_1000202225Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi216595.PFLU5410.

    Structurei

    3D structure databases

    ProteinModelPortaliC3K2L2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi627 – 6315"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C3K2L2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHEHYQPREI ENAAQSFWDE QKSFEVSEQP GKETFYCLSM FPYPSGKLHM    50
    GHVRNYTIGD VISRYQRMQG KNVLQPMGWD AFGMPAENAA MKNNVAPAKW 100
    TYENIAYMKN QLRSLGLAVD WSREVTTCKP DYYRWEQWLF TRLFEKGVIY 150
    KKSGTVNWDP IDQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFKITAYAD 200
    ELLSSLDDLP GWPEQVKTMQ RNWIGKSKGM EVQFPYNVDS IGEAGALKVF 250
    TTRPDTLMGA TYVAVAAEHP LATQAAQNNP ELQAFIAECK GGSVAEADVA 300
    TQEKKGLPTG LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF 350
    EFATKYSLPI KSVVRTSSGD TNPAPWQDAY GEHGELINSG EFDGLDFQGA 400
    FDAIEVALIK KNLGASRTQF RLRDWGISRQ RYWGCPIPII HCESCGDVPV 450
    PEDQLPVVLP EDVVPDGAGS PLARMPEFYE CSCPKCGQPA KRETDTMDTF 500
    VESSWYYARY ASPHYEGGLV EKSAADHWLP VDQYIGGIEH AILHLLYARF 550
    FHKLMRDEGL VSSNEPFKNL LTQGMVVAET YYRREANGAY TWFNPADVEL 600
    ERDSKAKVIS AKLIADGLPV EIGGTEKMAK SKNNGVDPQS MIDQFGADTC 650
    RLFMMFASPP DMSAEWSDSG VEGSHRFLKR VWRLAHAHIS QGLPGKLDVA 700
    ALSDEQKLIR RSIHLAIKQA SQDVGQNHKF NTAIAQVMTL MNVLEKAPQA 750
    TEQDRALVQE GLETVTLLLA PITPHISHDL WNRLGHSDAV IDARWPVQDD 800
    SALVQDTLQL VIQVNGKLRG QIDMPATASR EDVEAAARVN ENVLRFTEGL 850
    TIRKVIVVPG KLVNIVAS 868
    Length:868
    Mass (Da):97,128
    Last modified:June 16, 2009 - v1
    Checksum:i2870136D5A8A55C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM181176 Genomic DNA. Translation: CAY52580.1.
    RefSeqiYP_002874908.1. NC_012660.1.

    Genome annotation databases

    EnsemblBacteriaiCAY52580; CAY52580; PFLU_5410.
    GeneIDi7817534.
    KEGGipfs:PFLU5410.
    PATRICi19904920. VBIPseFlu98510_5532.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM181176 Genomic DNA. Translation: CAY52580.1 .
    RefSeqi YP_002874908.1. NC_012660.1.

    3D structure databases

    ProteinModelPortali C3K2L2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216595.PFLU5410.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAY52580 ; CAY52580 ; PFLU_5410 .
    GeneIDi 7817534.
    KEGGi pfs:PFLU5410.
    PATRICi 19904920. VBIPseFlu98510_5532.

    Phylogenomic databases

    eggNOGi COG0495.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SBW25.

    Entry informationi

    Entry nameiSYL_PSEFS
    AccessioniPrimary (citable) accession number: C3K2L2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3