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C3K2L2 (SYL_PSEFS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PFLU_5410
OrganismPseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP]
Taxonomic identifier216595 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202225

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif627 – 6315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C3K2L2 [UniParc].

Last modified June 16, 2009. Version 1.
Checksum: 2870136D5A8A55C1

FASTA86897,128
        10         20         30         40         50         60 
MHEHYQPREI ENAAQSFWDE QKSFEVSEQP GKETFYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMQG KNVLQPMGWD AFGMPAENAA MKNNVAPAKW TYENIAYMKN QLRSLGLAVD 

       130        140        150        160        170        180 
WSREVTTCKP DYYRWEQWLF TRLFEKGVIY KKSGTVNWDP IDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALIEKREIPM YYFKITAYAD ELLSSLDDLP GWPEQVKTMQ RNWIGKSKGM EVQFPYNVDS 

       250        260        270        280        290        300 
IGEAGALKVF TTRPDTLMGA TYVAVAAEHP LATQAAQNNP ELQAFIAECK GGSVAEADVA 

       310        320        330        340        350        360 
TQEKKGLPTG LFVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF EFATKYSLPI 

       370        380        390        400        410        420 
KSVVRTSSGD TNPAPWQDAY GEHGELINSG EFDGLDFQGA FDAIEVALIK KNLGASRTQF 

       430        440        450        460        470        480 
RLRDWGISRQ RYWGCPIPII HCESCGDVPV PEDQLPVVLP EDVVPDGAGS PLARMPEFYE 

       490        500        510        520        530        540 
CSCPKCGQPA KRETDTMDTF VESSWYYARY ASPHYEGGLV EKSAADHWLP VDQYIGGIEH 

       550        560        570        580        590        600 
AILHLLYARF FHKLMRDEGL VSSNEPFKNL LTQGMVVAET YYRREANGAY TWFNPADVEL 

       610        620        630        640        650        660 
ERDSKAKVIS AKLIADGLPV EIGGTEKMAK SKNNGVDPQS MIDQFGADTC RLFMMFASPP 

       670        680        690        700        710        720 
DMSAEWSDSG VEGSHRFLKR VWRLAHAHIS QGLPGKLDVA ALSDEQKLIR RSIHLAIKQA 

       730        740        750        760        770        780 
SQDVGQNHKF NTAIAQVMTL MNVLEKAPQA TEQDRALVQE GLETVTLLLA PITPHISHDL 

       790        800        810        820        830        840 
WNRLGHSDAV IDARWPVQDD SALVQDTLQL VIQVNGKLRG QIDMPATASR EDVEAAARVN 

       850        860 
ENVLRFTEGL TIRKVIVVPG KLVNIVAS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM181176 Genomic DNA. Translation: CAY52580.1.
RefSeqYP_002874908.1. NC_012660.1.

3D structure databases

ProteinModelPortalC3K2L2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216595.PFLU5410.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAY52580; CAY52580; PFLU_5410.
GeneID7817534.
KEGGpfs:PFLU5410.
PATRIC19904920. VBIPseFlu98510_5532.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSEFS
AccessionPrimary (citable) accession number: C3K2L2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries