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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Pseudomonas fluorescens (strain SBW25)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi487MagnesiumUniRule annotation1
Metal bindingi493MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:PFLU_5249
OrganismiPseudomonas fluorescens (strain SBW25)
Taxonomic identifieri216595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002332 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002156661 – 701Polyribonucleotide nucleotidyltransferaseAdd BLAST701

Proteomic databases

PRIDEiC3K255.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi216595.PFLU5249.

Structurei

3D structure databases

SMRiC3K255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini554 – 613KHUniRule annotationAdd BLAST60
Domaini623 – 691S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
KOiK00962.
OMAiRYMHNYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

C3K255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPVIKKFQF GQSTVTLETG RIARQASGAV LVTVDDDVTV LVTVVGAKTA
60 70 80 90 100
DPSKGFFPLS VHYQEKTYAA GKIPGGFFKR EGRPSEKETL TSRLIDRPIR
110 120 130 140 150
PLFPEGFMNE VQVVCTVVST SKKTDPDIAA MIGTSAALAI SGIPFDGPIG
160 170 180 190 200
AARVAFHEST GYLLNPTYEQ QKASSLDMVV AGTSEAVLMV ESEAKELTED
210 220 230 240 250
QMLGAVLFAH DEFQVVINAV KELAAEAAKP TWTWAPQPEA TALLGAIRSE
260 270 280 290 300
FGAAISDAYT ITVKADRYAR LGELKDQVVA KLSGEEGQPS SSEVKAAFGE
310 320 330 340 350
IEYRTVRENI VNGKPRIDGR DTRTVRPLNI EVGVLPKTHG SALFTRGETQ
360 370 380 390 400
ALVVATLGTA RDAQLLDTLE GEKKDPFMLH YNFPPFSVGE CGRMGGAGRR
410 420 430 440 450
EIGHGRLARR SISAMLPAAD VFPYTIRVVS EITESNGSSS MASVCGASLA
460 470 480 490 500
LMDAGVPMKA PVAGIAMGLV KEGQKFAILT DILGDEDHLG DMDFKVAGTA
510 520 530 540 550
KGVTALQMDI KIKGITEEIM EIALGQALEA RLNILGQMNQ IIGQSRTELS
560 570 580 590 600
ENAPTMIAMK IDTDKIRDVI GKGGATIRAI CEETKASIDI EDDGSIKIFG
610 620 630 640 650
ETKEAAEAAR QRVLGITAEA EIGKIYVGKV ERIVDFGAFV NILPGKDGLV
660 670 680 690 700
HISMLSDARV EKVTDILKEG QEVEVLVLDV DNRGRIKLSI KDVAAAKASG

V
Length:701
Mass (Da):74,936
Last modified:June 16, 2009 - v1
Checksum:iF34B7D32969F2231
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM181176 Genomic DNA. Translation: CAY52345.1.
RefSeqiWP_015885915.1. NC_012660.1.

Genome annotation databases

EnsemblBacteriaiCAY52345; CAY52345; PFLU_5249.
KEGGipfs:PFLU_5249.
PATRICifig|216595.4.peg.5381.

Similar proteinsi

Entry informationi

Entry nameiPNP_PSEFS
AccessioniPrimary (citable) accession number: C3K255
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: September 27, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families