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C3K1N4 (C3K1N4_PSEFS) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168 RuleBase RU003777
EC=2.4.2.29 HAMAP-Rule MF_00168 RuleBase RU003777
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168
Ordered Locus Names:PFLU_5077 EMBL CAY52078.1
OrganismPseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP] EMBL CAY52078.1
Taxonomic identifier216595 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS004803

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS004803

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. HAMAP-Rule MF_00168 RuleBase RU003777

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site921Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3031Zinc By similarity HAMAP-Rule MF_00168
Metal binding3051Zinc By similarity HAMAP-Rule MF_00168
Metal binding3081Zinc By similarity HAMAP-Rule MF_00168
Metal binding3341Zinc By similarity HAMAP-Rule MF_00168
Binding site931Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
C3K1N4 [UniParc].

Last modified June 16, 2009. Version 1.
Checksum: DE7B799AEA0BF4D0

FASTA37441,425
        10         20         30         40         50         60 
MSRMSFELLA TDGKARRGRL TFPRGTVETP AFMPVGTYGT VKGMLPRDIV ATGAEIILGN 

        70         80         90        100        110        120 
TFHLWLRPGT EVIKKHGDLH DFMKWQGPIL TDSGGFQVFS LGAMRKIKEE GVTFASPVDG 

       130        140        150        160        170        180 
SKVFMGPEES MQVQRDLGSD IVMIFDECTP YPADEDVARI SMELSLRWAQ RSKNAHGDNT 

       190        200        210        220        230        240 
AALFGIVQGG MHESLRKRSL EGLDKIGFDG LAIGGLSVGE PKHEMIKVLD YLPGLMPADK 

       250        260        270        280        290        300 
PRYLMGVGKP EDLVEGVRRG VDMFDCVMPT RNARNGHLFI DTGVLKIRNA FHRHDDSPLD 

       310        320        330        340        350        360 
PTCDCYTCQN FSRAYLHHLD KCGEMLGSML NTIHNLRHYQ VLMAGLREAI QQGTLAAFVD 

       370 
AFYAKRGLPV PPLD

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM181176 Genomic DNA. Translation: CAY52078.1.
RefSeqYP_002874580.1. NC_012660.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING216595.PFLU5077.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAY52078; CAY52078; PFLU_5077.
GeneID7819577.
KEGGpfs:PFLU5077.
PATRIC19904264. VBIPseFlu98510_5212.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
KOK00773.
OMAMSPERSI.
ProtClustDBPRK00112.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC3K1N4_PSEFS
AccessionPrimary (citable) accession number: C3K1N4
Entry history
Integrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: May 29, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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