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C3HP06 (C3HP06_BACTU) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP MF_00036
EC=6.1.1.7 HAMAP MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP MF_00036
Gene names
Name:alaS HAMAP MF_00036
ORF Names:bthur0012_42340 EMBL EEM87717.1
OrganismBacillus thuringiensis serovar pulsiensis BGSC 4CC1 EMBL EEM87717.1
Taxonomic identifier527028 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length880 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036

Subcellular location

Cytoplasm By similarity HAMAP MF_00036.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding5671Zinc By similarity HAMAP MF_00036
Metal binding5711Zinc By similarity HAMAP MF_00036
Metal binding6691Zinc By similarity HAMAP MF_00036
Metal binding6731Zinc By similarity HAMAP MF_00036

Sequences

Sequence LengthMass (Da)Tools
C3HP06 [UniParc].

Last modified June 16, 2009. Version 1.
Checksum: 7D59661D071ED760

FASTA88097,473
        10         20         30         40         50         60 
MKQLTGAQIR QMFLDFFQEK GHAVEPSASL VPHEDPSLLW INSGVATLKK YFDGRVIPQN 

        70         80         90        100        110        120 
PRITNAQKSI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEEAITWAWE FLTSDKWIGF 

       130        140        150        160        170        180 
DKELLSVTIH PEDEEAFTIW NEKMGVPKER IIRLEENFWD IGEGPSGPNT EIFYDRGEAY 

       190        200        210        220        230        240 
GNDFSDPELY PGGENERYLE VWNLVFSQFN HNPDGSYTPL PKKNIDTGMG LERMTSIVQD 

       250        260        270        280        290        300 
VPTNFDTDLF MPMIGATETI SGEKYRNGDL EKDMAFKVIA DHIRTVTFAV GDGALPSNEG 

       310        320        330        340        350        360 
RGYVLRRLLR RAVRYSKKLN INRPFMFELV PVVGEVMKDF YPEVLEKKDF IAKVVKNEEE 

       370        380        390        400        410        420 
RFHETLHDGE AILAEVIAKA KEEKTTVISG VDAFRLYDTY GFPIELTEEY AEEAGMTVDH 

       430        440        450        460        470        480 
EGFENEMEKQ RERARAARQD VDSMQVQGGV LGEVKVASEF VGYGTVATES NVVALVKNGE 

       490        500        510        520        530        540 
YTDSLQVGEE GQLMLDVTPF YAESGGQIAD RGYLLADGVK VLVKDVQKAP NGQNLHKVVV 

       550        560        570        580        590        600 
EEGTLTKDAA VKAIIDTKNR SSVVKNHTAT HLLHQALKDV LGTHVNQAGS LVTSERLRFD 

       610        620        630        640        650        660 
FSHFGQVQAD ELEKIERIVN EKIWESIDVE ISQKAIEEAK EMGAMALFGE KYGDVVRVVQ 

       670        680        690        700        710        720 
VGDYSLELCG GCHVDNTASI GIFKIVAESG IGAGTRRIEA VTGKSAYELM NDQVGLLKEA 

       730        740        750        760        770        780 
AGKMKTNPKD ILTRVDGLFA EVKQLQKENE SLAAKLSNIE AGNLTDSVMT VDGVNVLAAK 

       790        800        810        820        830        840 
VNVADMNNLR TMMDDLKNKL ESAVVVLASV NDDKVNILAG VTKDLISQGY HAGKLVKEVA 

       850        860        870        880 
SRCGGGGGGR PDMAQAGGKN PAQVEEALAF VQEYVKSVSK

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References

[1]"Annotation of the Bacillus thuringiensis BGSC 4CC1 genome."
Read T.D., Akmal A., Bishop-Lilly K., Chen P.E., Cook C., Kiley M.P., Lentz S., Mateczun A., Nagarajan N., Nolan N., Osborne B.I., Pop M., Sozhamannan S., Stewart A.C., Sulakvelidze A., Thomason B., Willner K., Zwick M.E.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BGSC 4CC1 EMBL EEM87717.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		ACNJ01000087 Genomic DNA. Translation: EEM87717.1.

3D structure databases

ProteinModelPortalC3HP06.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000183595; EBBACP00000178897; EBBACG00000184047.
PATRIC28947894. VBIBacThu66700_5683.

Family and domain databases

HAMAPMF_00036_B. Ala_tRNA_synth_B.
[Tree]
InterProIPR002318. Ala-tRNA-synth_IIc.
IPR018162. Ala-tRNA-synth_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_synth_euk/bac.
IPR003156. Pesterase_DHHA1.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR00344. AlaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC3HP06_BACTU
AccessionPrimary (citable) accession number: C3HP06
Entry history
Integrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info