C3ACA9 (C3ACA9_BACMY) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase HAMAP MF_00091
EC=4.4.1.21 HAMAP MF_00091

Alternative name(s):
AI-2 synthesis protein HAMAP MF_00091
Autoinducer-2 production protein LuxS HAMAP MF_00091

Gene names

Name:	luxS HAMAP MF_00091
ORF Names:	bmyco0001_44010 EMBL EEL97128.1

Organism

Bacillus mycoides DSM 2048 EMBL EEL97128.1

Taxonomic identifier

526997 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091 SAAS SAAS003815
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091 SAAS SAAS003815
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091 SAAS SAAS003815
Subunit structure	Homodimer By similarity. HAMAP MF_00091 SAAS SAAS003815
Sequence similarities	Belongs to the LuxS family. HAMAP MF_00091

Ontologies

Keywords
Biological process	Autoinducer synthesis HAMAP MF_00091 SAAS SAAS003815 Quorum sensing HAMAP MF_00091 SAAS SAAS003815
Ligand	Iron HAMAP MF_00091 SAAS SAAS003815 Metal-binding HAMAP MF_00091 SAAS SAAS003815
Molecular function	Lyase HAMAP MF_00091 SAAS SAAS003815 EMBL EEL97128.1
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	54	1	Iron By similarity HAMAP MF_00091
	Metal binding	58	1	Iron By similarity HAMAP MF_00091
	Metal binding	126	1	Iron By similarity HAMAP MF_00091

Sequences

	Sequence		Length	Mass (Da)
	C3ACA9 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 5D2BB8E3B7154793	FASTA	157	17,855
	10 20 30 40 50 60 MPSVESFELD HTIVKAPYVR HCGVHNVGSD GIVNKFDIRF CQPNKQAMKP DVIHTLEHLL 70 80 90 100 110 120 AFNLRKYIDR YPHFDIIDIS PMGCQTGYYL VVSGTPTVRE IIDLLELTLK DAVQITEIPA 130 140 150 ANETQCGQAK LHDLEGAQRL MNFWLSQDKD ELEKVFG

References

[1]

"Annotation of the Bacillus mycoides DSM 2048 genome." Read T.D., Akmal A., Bishop-Lilly K., Chen P.E., Cook C., Kiley M.P., Lentz S., Mateczun A., Nagarajan N., Nolan N., Osborne B.I., Pop M., Sozhamannan S., Stewart A.C., Sulakvelidze A., Thomason B., Willner K., Zwick M.E. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 2048 EMBL EEL97128.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACMU01000084 Genomic DNA. Translation: EEL97128.1.
3D structure databases
ProteinModelPortal	C3ACA9.
SMR	C3ACA9. Positions 4-157.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000253359; EBBACP00000243612; EBBACG00000252538.
PATRIC	25164504. VBIBacMyc112645_5121.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C3ACA9_BACMY

Accession

Primary (citable) accession number: C3ACA9

Entry history

Integrated into UniProtKB/TrEMBL:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	December 14, 2011
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)