C2TF82 (C2TF82_BACCE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase 1 HAMAP MF_00488
Short name=L-LDH 1 HAMAP MF_00488
EC=1.1.1.27 HAMAP MF_00488

Gene names

Name:	ldh1 HAMAP MF_00488
ORF Names:	bcere0016_18080 EMBL EEL17581.1

Organism

Bacillus cereus 95/8201 EMBL EEL17581.1

Taxonomic identifier

526979 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family. HAMAP MF_00488

Ontologies

Keywords
Biological process	Glycolysis HAMAP MF_00488
Cellular component	Cytoplasm HAMAP MF_00488
Ligand	NAD HAMAP MF_00488
Molecular function	Oxidoreductase HAMAP MF_00488
PTM	Phosphoprotein HAMAP MF_00488
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity HAMAP MF_00488
Sites
	Active site	178	1	Proton acceptor By similarity HAMAP MF_00488
	Binding site	91	1	Substrate By similarity HAMAP MF_00488
	Binding site	123	1	NAD or substrate By similarity HAMAP MF_00488
	Binding site	154	1	Substrate By similarity HAMAP MF_00488
	Binding site	232	1	Substrate By similarity HAMAP MF_00488
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity HAMAP MF_00488

Sequences

	Sequence		Length	Mass (Da)
	C2TF82 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: A99C911C2A2306E6	FASTA	314	34,788
	10 20 30 40 50 60 MKKGINRVVL VGTGAVGCSY AYCMINQAVA EEFVLVDVNE AKAEGEAMDL SHAVPFAPAP 70 80 90 100 110 120 TRVWKGSYED CKDADLVVIT AGLPQKPGET RLDLVEKNAK IFKQIVRSIM DSGFDGIFLI 130 140 150 160 170 180 ATNPVDILTY VTWKESGLPK ERVIGSGTTL DSARFRYMLG EYFNIGPHNI HAYIIGEHGD 190 200 210 220 230 240 TELPVWSHVS VGIQKLQTLL EKDNTYNQED LDKIFINVRD AAYHIIERKG ATYYGIGMSL 250 260 270 280 290 300 LRVTKAILND ENSVLTVSAY LEGQYGQKDV YIGVPAVLNR GGVREILEVE LSEDEELKFD 310 HSVQVLKETM APVL

References

[1]

"Annotation of the Bacillus cereus 95/8201 genome." Read T.D., Akmal A., Bishop-Lilly K., Chen P.E., Cook C., Kiley M.P., Lentz S., Mateczun A., Nagarajan N., Nolan N., Osborne B.I., Pop M., Sozhamannan S., Stewart A.C., Sulakvelidze A., Thomason B., Willner K., Zwick M.E. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 95/8201 EMBL EEL17581.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACMF01000038 Genomic DNA. Translation: EEL17581.1.
3D structure databases
ProteinModelPortal	C2TF82.
SMR	C2TF82. Positions 4-311.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000190526; EBBACP00000180949; EBBACG00000186173.
PATRIC	24764594. VBIBacCer42905_2075.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF3. PTHR11540:SF3. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C2TF82_BACCE

Accession

Primary (citable) accession number: C2TF82

Entry history

Integrated into UniProtKB/TrEMBL:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)