C2S9A9 (C2S9A9_BACCE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684
Short name=MTA/SAH nucleosidase HAMAP MF_01684
Short name=MTAN HAMAP MF_01684
EC=3.2.2.9 HAMAP MF_01684

Alternative name(s):
5'-methylthioadenosine nucleosidase HAMAP MF_01684
S-adenosylhomocysteine nucleosidase HAMAP MF_01684

Gene names

Name:	mtnN HAMAP MF_01684
ORF Names:	bcere0013_42050 EMBL EEK98678.1

Organism

Bacillus cereus BDRD-ST26 EMBL EEK98678.1

Taxonomic identifier

526975 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684 SAAS SAAS010049 S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684 SAAS SAAS010049
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily. HAMAP MF_01684

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis HAMAP MF_01684 SAAS SAAS010049
Molecular function	Hydrolase HAMAP MF_01684 SAAS SAAS010049
Gene Ontology (GO)
Biological process	L-methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: HAMAP methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	174 – 175	2	Substrate binding By similarity HAMAP MF_01684
Sites
	Active site	12	1	Proton acceptor By similarity HAMAP MF_01684
	Binding site	78	1	Substrate; via amide nitrogen By similarity HAMAP MF_01684
	Binding site	198	1	Substrate By similarity HAMAP MF_01684

Sequences

	Sequence		Length	Mass (Da)
	C2S9A9 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 11F3ACE054DE48AC	FASTA	231	25,255
	10 20 30 40 50 60 MRIAVIGAME EEVRILRDKL EQAETETVAG CEFTKGQLAG HEVILLKSGI GKVNAAMSTT 70 80 90 100 110 120 ILLERYKPEK VINTGSAGGF HHSLNVGDVV ISTEVRHHDV DVTAFNYEYG QVPGMPPGFK 130 140 150 160 170 180 ADEALVALAE KCMQAEENIQ VVKGMIATGD SFMSDPNRVA AIRDKFENLY AVEMEAAAVA 190 200 210 220 230 QVCHQYEVPF VIIRALSDIA GKESNVSFDQ FLDQAALHST NFIVKVLEEL K

References

[1]

"Annotation of the Bacillus cereus BDRD-ST26 genome." Read T.D., Akmal A., Bishop-Lilly K., Chen P.E., Cook C., Kiley M.P., Lentz S., Mateczun A., Nagarajan N., Nolan N., Osborne B.I., Pop M., Sozhamannan S., Stewart A.C., Sulakvelidze A., Thomason B., Willner K., Zwick M.E. Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: BDRD-ST26 EMBL EEK98678.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACMC01000086 Genomic DNA. Translation: EEK98678.1.
3D structure databases
ProteinModelPortal	C2S9A9.
SMR	C2S9A9. Positions 1-230.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000202241; EBBACP00000197147; EBBACG00000207095.
PATRIC	24923889. VBIBacCer77638_5347.
Family and domain databases
HAMAP	MF_01684. Salvage_tnN. [Tree]
InterPro	IPR010049. MTA_SAH_Nsdase. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
PANTHER	PTHR21234. PNP_UDP. 1 hit. PTHR21234:SF6. PTHR21234:SF6. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C2S9A9_BACCE

Accession

Primary (citable) accession number: C2S9A9

Entry history

Integrated into UniProtKB/TrEMBL:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	December 14, 2011
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)