C1P616 (IBSD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small toxic protein IbsD

Gene names

Name:	ibsD
Ordered Locus Names:	b4664, JW3023.1

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	19 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be a toxic protein; overexpression causes cessation of growth.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Induction	The sibD sRNA probably represses expression of ibsD mRNA, either by destabilizing the transcript and/or preventing its translation Probable. A type I toxin antitoxin (TA) system, where expression of the proteinaceous toxin is controlled by an antisense sRNA.
Disruption phenotype	None seen. An isbD overproducing strain cannot be made in the absence of the sibD gene. Ref.3
Miscellaneous	Part of the SIBd repeat region (formerly known as QUAD1d), encoded on the opposite strand from the sibD (formerly known as rygD) RNA.
Sequence similarities	Belongs to the toxic protein ibs family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 19	19	Small toxic protein IbsD		PRO_0000386423
Regions
	Transmembrane	4 – 18	15	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	C1P616 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 7937D62BC3D5A8F7	FASTA	19	2,151
	10 MMKLVIILIV LLLVSFAAY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Repression of small toxic protein synthesis by the Sib and OhsC small RNAs." Fozo E.M., Kawano M., Fontaine F., Kaya Y., Mendieta K.S., Jones K.L., Ocampo A., Rudd K.E., Storz G. Mol. Microbiol. 70:1076-1093(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: ACO60008.1. AP009048 Genomic DNA. No translation available.
RefSeq	YP_002791256.1. NC_000913.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACO60008; ACO60008; b4664.
GeneID	7751628.
KEGG	eco:b4664.
Organism-specific databases
EcoGene	EG14469. ibsD.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-2856.
Gene expression databases
Genevestigator	C1P616.
Family and domain databases
InterPro	IPR025881. Toxin_Ibs. [Graphical view]
Pfam	PF13956. Ibs_toxin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IBSD_ECOLI

Accession

Primary (citable) accession number: C1P616

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 13, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program