C1KYG8 (C1KYG8_LISMC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1 HAMAP MF_00108
MEP cytidylyltransferase 1 HAMAP MF_00108

Gene names

Name:	ispD1 HAMAP MF_00108
Ordered Locus Names:	Lm4b_00255

Organism

Listeria monocytogenes serotype 4b (strain Clip81459) [Complete proteome] [HAMAP]

Taxonomic identifier

568819 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU003490
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU003490
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393
Molecular function	Nucleotidyltransferase SAAS SAAS018294 HAMAP MF_00108 RuleBase RU004393 EMBL CAS04023.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	15	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	22	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	153	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	209	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	C1KYG8 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: B0A8E3D1681A0ED7	FASTA	232	25,898
	10 20 30 40 50 60 MNYELVFLAA GQGKRMNAQK NKMWLELVGE PIFIHALRPF LADNRCSKVI VVSQEEERKH 70 80 90 100 110 120 VKELMSQLNV AESRIEIVKG GSERQYSVAA GLERCGTERV VLVHDGARPF ITLDIIDRLL 130 140 150 160 170 180 IGVEQSKAAI CAVKVKDTVK RVVKGVVQET VDRENLWQVQ TPQAFELPIL RKAHQLARKE 190 200 210 220 230 QFLGTDEASL VERLPCPVAI VQGSYYNIKL TTPEDMPLAK AILGELGGIA ND

References

[1]	"Complete genome sequence of Listeria monocytogenes serotype 4b strain Clip81459." Rusniok C. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM242711 Genomic DNA. Translation: CAS04023.1.
RefSeq	YP_002756971.1. NC_012488.1.
3D structure databases
ProteinModelPortal	C1KYG8.
ModBase	Search...
Protein-protein interaction databases
STRING	C1KYG8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7703659.
GenomeReviews	Gene locus Lm4b_00255 in contig FM242711_GR.
KEGG	lmc:Lm4b_00255.
PATRIC	20303612. VBILisMon88360_0242.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SKVIVVC.
ProtClustDB	PRK13385.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C1KYG8_LISMC

Accession

Primary (citable) accession number: C1KYG8

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)