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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Listeria monocytogenes serotype 4b (strain CLIP80459)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Lm4b_02031
OrganismiListeria monocytogenes serotype 4b (strain CLIP80459)
Taxonomic identifieri568819 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000002203: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 921921Isoleucine--tRNA ligasePRO_1000216240Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi634178.Lm4b_02031.

Structurei

3D structure databases

ProteinModelPortaliC1KWW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1KWW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYKDTLLMP KTDFPMRGNL PNKEPEWQAK WEEEKLYEKI QEKNAGRKAY
60 70 80 90 100
ILHDGPPYAN GELHMGHALN KTIKDIIVRY KSMAGFSSPY VPGWDTHGLP
110 120 130 140 150
IETAIAKKGV KRKEMSIAEF RKLCAEYAMT QVDGQRTGFK RLGINGDWEN
160 170 180 190 200
PYITLLPEYE AEQIKVFGEM AKKGYIYKGK KPVYWSPSSE SALAEAEIEY
210 220 230 240 250
QDKKSASIFV AFKVTDGKGV LDEGTNIVIW TTTPWTIPAN MGITVNPDLD
260 270 280 290 300
YVVIESAGEK YVVAEALLPS LREKLGFEDA TVVKTVRGSE LDRVVTKHPF
310 320 330 340 350
YDRDSLVMNG EHATAEAGTG AVHTAPGHGE DDFLIGKKYD LEVLAPLDDR
360 370 380 390 400
GVFTEEAPGF EGVFYDTANK MVTEKLEEVG ALLKMEFITH SYPHDWRTKK
410 420 430 440 450
PVIFRATAQW FASIDAFRDD LLAAVKGVNW TPAWGETRLF NMVRDRGDWV
460 470 480 490 500
ISRQRAWGVP LPIFYAENGE AIITDETINH ISELFREHGS NVWFERDVKD
510 520 530 540 550
LLPAGFTHPG SPNGEFTKET DIMDVWFDSG SSHQAVLNAR PELSRPADLY
560 570 580 590 600
MEGSDQYRGW FNSSLTTAVA ITGEAPYRNV LSHGFALDGE GRKMSKSLGN
610 620 630 640 650
TLLPGKVIKQ LGADIVRLWV ASVDYQADVR VSDEILKQVS EVYRKIRNTM
660 670 680 690 700
RFLLGNINDF NPTTNAVSYE NLREVDKYML IKLNDLVKNV KDNYEAFEFS
710 720 730 740 750
TIYHQINNFC TVELSQFYMD FAKDVVYIEA ADSHDRRAMQ TVFYEAVVTL
760 770 780 790 800
TKLLAPILPH TTEEVWNSLI GEGVESIHLQ DLPEVKVLAD SEEITAKWDA
810 820 830 840 850
FMQIRDNVQK ALEFARNEKL IGKSMLAKVT LYVDGEAKTL FDSLEGDFAQ
860 870 880 890 900
LFIVSDFELV EGLENAPESA FKSNQVAVQI TVAEGETCER CRVVKKDVGV
910 920
NPKHPTLCGR CADIVVKHYE A
Length:921
Mass (Da):103,878
Last modified:May 26, 2009 - v1
Checksum:i8018C3A3CCBF3ABF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM242711 Genomic DNA. Translation: CAS05789.1.
RefSeqiYP_002758723.1. NC_012488.1.

Genome annotation databases

EnsemblBacteriaiCAS05789; CAS05789; Lm4b_02031.
GeneIDi7703975.
KEGGilmc:Lm4b_02031.
PATRICi20307299. VBILisMon88360_2048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM242711 Genomic DNA. Translation: CAS05789.1.
RefSeqiYP_002758723.1. NC_012488.1.

3D structure databases

ProteinModelPortaliC1KWW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi634178.Lm4b_02031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAS05789; CAS05789; Lm4b_02031.
GeneIDi7703975.
KEGGilmc:Lm4b_02031.
PATRICi20307299. VBILisMon88360_2048.

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIP80459.

Entry informationi

Entry nameiSYI_LISMC
AccessioniPrimary (citable) accession number: C1KWW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: January 7, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.