C1KWT1 (LEU1_LISMC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-isopropylmalate synthase
EC=2.3.3.13

Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase

Gene names

Name:	leuA
Ordered Locus Names:	Lm4b_01999

Organism

Listeria monocytogenes serotype 4b (strain CLIP80459) [Complete proteome] [HAMAP]

Taxonomic identifier

568819 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_01025
Catalytic activity	Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_01025
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_01025
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-isopropylmalate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	2-isopropylmalate synthase HAMAP-Rule MF_01025		PRO_1000213315

Sequences

	Sequence		Length	Mass (Da)
	C1KWT1 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 475D88C6E46318FF	FASTA	512	56,064
	10 20 30 40 50 60 MKKIQFFDTT LRDGEQTPGV NFDVKEKIQI ALQLEKLGID VIEAGFPISS PGDFECVKAI 70 80 90 100 110 120 AKAIKHCSVT GLARCVEGDI DRAEEALKDA VSPQIHIFLA TSDVHMEYKL KMSRAEVLAS 130 140 150 160 170 180 IKHHISYARQ KFDVVQFSPE DATRSDRAFL IEAVQTAIDA GATVINIPDT VGYTNPTEFG 190 200 210 220 230 240 QLFQDLRREI KQFDDIIFAS HCHDDLGMAT ANALAAIENG ARRVEGTING IGERAGNTAL 250 260 270 280 290 300 EEVAVALHIR KDFYQAETNI VLNQFKNSSD LISRLSGMPV PRNKAVIGGN AYAHESGIHQ 310 320 330 340 350 360 DGVLKNPDTY EIITPALVGV DKNSLPLGKL SGKHAFNTRM EEMGYTLTEQ EQKDAFKRFK 370 380 390 400 410 420 QLADAKKEVT EEDLHALILG QSSESADDFE LKHLQVQYVT GGVQGAIVRI EERDGALVED 430 440 450 460 470 480 AATGSGSIEA IYNTINRLMK QDIELTDYRI QAITAGQDAQ AEVHVVIKND KGAVFHGIGI 490 500 510 DFDVLTASAK AYLQASGKSK TASKQADFEE VK

References

[1]

"Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes." Hain T., Ghai R., Billion A., Kuenne C.T., Steinweg C., Izar B., Mohamed W., Mraheil M., Domann E., Schaffrath S., Karst U., Goesmann A., Oehm S., Puhler A., Merkl R., Vorwerk S., Glaser P., Garrido P. , Rusniok C., Buchrieser C., Goebel W., Chakraborty T. BMC Genomics 13:144-144(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP80459.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM242711 Genomic DNA. Translation: CAS05756.1.
RefSeq	YP_002758692.1. NC_012488.1.
3D structure databases
ProteinModelPortal	C1KWT1.
ModBase	Search...
Protein-protein interaction databases
STRING	634178.Lm4b_01999.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAS05756; CAS05756; Lm4b_01999.
GeneID	7702506.
KEGG	lmc:Lm4b_01999.
PATRIC	20307229. VBILisMon88360_2013.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000046859.
KO	K01649.
OMA	VWSVHCH.
ProtClustDB	PRK00915.
Enzyme and pathway databases
BioCyc	LMON568819:GJF9-2955-MONOMER.
UniPathway	UPA00048; UER00070.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01025. LeuA_type1.
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR005671. LeuA_bact_synth. IPR000891. PYR_CT. [Graphical view]
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR00973. leuA_bact. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU1_LISMC

Accession

Primary (citable) accession number: C1KWT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program