ID   FTHS_LISMC              Reviewed;         560 AA.
AC   C1KWH6;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   27-MAR-2024, entry version 64.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Lm4b_01893;
OS   Listeria monocytogenes serotype 4b (strain CLIP80459).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=568819;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIP80459;
RX   PubMed=22530965; DOI=10.1186/1471-2164-13-144;
RA   Hain T., Ghai R., Billion A., Kuenne C.T., Steinweg C., Izar B.,
RA   Mohamed W., Mraheil M., Domann E., Schaffrath S., Karst U., Goesmann A.,
RA   Oehm S., Puhler A., Merkl R., Vorwerk S., Glaser P., Garrido P.,
RA   Rusniok C., Buchrieser C., Goebel W., Chakraborty T.;
RT   "Comparative genomics and transcriptomics of lineages I, II, and III
RT   strains of Listeria monocytogenes.";
RL   BMC Genomics 13:144-144(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; FM242711; CAS05651.1; -; Genomic_DNA.
DR   RefSeq; WP_003728274.1; NC_012488.1.
DR   AlphaFoldDB; C1KWH6; -.
DR   SMR; C1KWH6; -.
DR   KEGG; lmc:Lm4b_01893; -.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   UniPathway; UPA00193; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..560
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000215438"
FT   BINDING         69..76
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   560 AA;  60126 MW;  B0D92B76E1DF66BC CRC64;
     MSNKVKSDIE IASKAEILPV TTIAEHLGLD ADALELYGKY KAKLSYDTIH SLKDKEPGKL
     VLVTAINPTP AGEGKSTVTV GLGDALSKKD KKTVIALREP SLGPTMGIKG GATGGGYAQV
     IPMEDINLHF TGDFHAITAA NNALSAFIDN HMQQGNDLDI DGRRIVWKRV VDLNDRALRK
     VVVGLGGPIQ GVPREDGFDI TVASEIMAII CLASDLKDLK KRLSEIVIGY NYKKEPITVG
     EMGYEGALTL LLKDALKPNL VQTLEHTPAI VHGGPFANIA HGCNSVSATS TALRLGEYVV
     TEAGFGADLG AEKFLDIKVP ALGKAPDCVV IVATIRALKM HGGALKTELS EENVDALAKG
     FTNLQKHTES IQTFGIPYVV AINKFITDSD AEVAKLEALC EEHGIPFSLT EVWEKGGDGG
     LELADKVIAA VESGEADYKR IYDDAWSIEE KLEAIVTKVY GGIGVELSSK AQKQIVEFKK
     YGWDRYPICM AKTQYSLSDD PTLLGRPTDF VIHIREFIPK LGAGFVVALT GDVMTMPGLP
     KKPAALNMDV DENGNAQGLF
//