C1KWA4 (RNC_LISMC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	Lm4b_01821

Organism

Listeria monocytogenes serotype 4b (strain CLIP80459) [Complete proteome] [HAMAP]

Taxonomic identifier

568819 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_1000202839
Regions
	Domain	4 – 133	130	RNase III
	Domain	159 – 228	70	DRBM
Sites
	Active site	50	1	Potential
	Active site	122	1	By similarity
	Metal binding	46	1	Magnesium By similarity
	Metal binding	119	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1KWA4 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 33D89A85F461F322	FASTA	229	25,905
	10 20 30 40 50 60 MNQWEELQES VGFDFKDVEL LKQAFTHSSY VNEHRRENVK DNERLEFLGD AVLELTVSNY 70 80 90 100 110 120 LFNKYPDMAE GHMTKMRAAI VCEPSLVEFA EAVHFSKYVR LGKGEEKAGG RTRPALLADV 130 140 150 160 170 180 FESFIGALYL DNGIDKVVTF LERVIFPKID AGAYLQTVDY KTQLQEIVQR DRDVLIEYDI 190 200 210 220 LGETGPAHNK AFDAQVIVNG QVLGKGSGRT KKQAEQSAAQ FAINQLTHR

References

[1]

"Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes." Hain T., Ghai R., Billion A., Kuenne C.T., Steinweg C., Izar B., Mohamed W., Mraheil M., Domann E., Schaffrath S., Karst U., Goesmann A., Oehm S., Puhler A., Merkl R., Vorwerk S., Glaser P., Garrido P. , Rusniok C., Buchrieser C., Goebel W., Chakraborty T. BMC Genomics 13:144-144(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP80459.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM242711 Genomic DNA. Translation: CAS05579.1.
RefSeq	YP_002758515.1. NC_012488.1.
3D structure databases
ProteinModelPortal	C1KWA4.
ModBase	Search...
Protein-protein interaction databases
STRING	634178.Lm4b_01821.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAS05579; CAS05579; Lm4b_01821.
GeneID	7701589.
KEGG	lmc:Lm4b_01821.
PATRIC	20306873. VBILisMon88360_1835.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000246808.
KO	K03685.
OMA	LTHKSCK.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	LMON568819:GJF9-2712-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_LISMC

Accession

Primary (citable) accession number: C1KWA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program