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C1KVL8

- PFKA_LISMC

UniProt

C1KVL8 - PFKA_LISMC

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, Lm4b_01582
Organism
Listeria monocytogenes serotype 4b (strain CLIP80459)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarity
Metal bindingi103 – 1031Magnesium; catalytic By similarity
Active sitei127 – 1271Proton acceptor By similarity
Binding sitei154 – 1541Allosteric activator ADP By similarity
Binding sitei162 – 1621Substrate; shared with dimeric partner By similarity
Binding sitei211 – 2111Allosteric activator ADP By similarity
Binding sitei222 – 2221Substrate By similarity
Binding sitei243 – 2431Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarity
Nucleotide bindingi102 – 1054ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:Lm4b_01582
OrganismiListeria monocytogenes serotype 4b (strain CLIP80459)
Taxonomic identifieri568819 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000002203: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_1000205249Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi634178.Lm4b_01582.

Structurei

3D structure databases

ProteinModelPortaliC1KVL8.
SMRiC1KVL8. Positions 1-319.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni125 – 1273Substrate binding By similarity
Regioni169 – 1713Substrate binding By similarity
Regioni185 – 1873Allosteric activator ADP binding By similarity
Regioni213 – 2153Allosteric activator ADP binding By similarity
Regioni249 – 2524Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiGFGGRCV.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1KVL8-1 [UniParc]FASTAAdd to Basket

« Hide

MKRIAILTSG GDAPGMNAAT RAVVRKAIYE GLEVYGINYG FLGLVNGDIR    50
KLELGSVGDL LHRGGTFLYS ARYPEFATEE GQLKGIEQLK KHQIDGLVVI 100
GGDGSYHGAE ALTKRGFPTI GIPGTIDNDI SGTDFTIGFD TALNTVLDAL 150
DKIRDTATSH ERTFIIEVMG RDAGDIALWS GLAGGAEAII VPEESFNMDD 200
VVDRLNKGRE RGKKHSIIVV AEGVMSGNEF AKQLAEYGDY HARVTVLGHV 250
QRGGSPTAFD RVLASRLGAR SVELLLENRG GLAVGIRENR IVENDISEIL 300
KEKHTLDQKL FDLASILSI 319
Length:319
Mass (Da):34,420
Last modified:May 26, 2009 - v1
Checksum:iAE15D577691BA74F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM242711 Genomic DNA. Translation: CAS05343.1.
RefSeqiYP_002758279.1. NC_012488.1.

Genome annotation databases

EnsemblBacteriaiCAS05343; CAS05343; Lm4b_01582.
GeneIDi7704091.
KEGGilmc:Lm4b_01582.
PATRICi20306331. VBILisMon88360_1592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM242711 Genomic DNA. Translation: CAS05343.1 .
RefSeqi YP_002758279.1. NC_012488.1.

3D structure databases

ProteinModelPortali C1KVL8.
SMRi C1KVL8. Positions 1-319.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 634178.Lm4b_01582.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAS05343 ; CAS05343 ; Lm4b_01582 .
GeneIDi 7704091.
KEGGi lmc:Lm4b_01582.
PATRICi 20306331. VBILisMon88360_1592.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi GFGGRCV.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIP80459.

Entry informationi

Entry nameiPFKA_LISMC
AccessioniPrimary (citable) accession number: C1KVL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: July 9, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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