Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyprotein

Gene
N/A
Organism
Solenopsis invicta virus 3 (SINV-3)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Capsid protein VP1: Assembles with VP1-FSD and VP2 to form an icosahedral capsid. VP1 is about 5 time more abundant than VP1-FSD in the virion.1 Publication
RNA-directed RNA polymerase: Replicates genomic and antigenomic RNA.PROSITE-ProRule annotationBy similarity

Miscellaneous

Capsid protein VP1: A sgRNA is also expressed which encloses the VP1 capsid protein and probably a leader protein.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1258For 3C-like protease1 Publication1
Active sitei1309For 3C-like protease1 Publication1
Active sitei1381For 3C-like protease1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi396 – 403ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.48. 11653.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprotein
Cleaved into the following 4 chains:
Helicase1 Publication (EC:3.6.4.13Curated)
3C-like protease1 Publication (EC:3.4.22.-Curated)
Short name:
3CL-PRO
RNA-directed RNA polymerasePROSITE-ProRule annotation (EC:2.7.7.48PROSITE-ProRule annotation)
Capsid protein VP11 Publication
OrganismiSolenopsis invicta virus 3 (SINV-3)
Taxonomic identifieri631345 [NCBI]
Taxonomic lineageiVirusesunassigned virusesInvictavirus
Proteomesi
  • UP000207613 Componenti: Genome

Subcellular locationi

Capsid protein VP1 :
  • Virion 1 Publication

GO - Cellular componenti

  • virion Source: UniProtKB-SubCell

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000442442? – ?14323C-like protease
ChainiPRO_00004424401 – 2580PolyproteinAdd BLAST2580
ChainiPRO_00004424411 – ?Helicase
ChainiPRO_0000442443?1433 – 2345RNA-directed RNA polymeraseAdd BLAST913
ChainiPRO_00004424442346 – 2580Capsid protein VP1Add BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2346N-acetylmethionine; by host1 Publication1

Post-translational modificationi

Capsid protein VP1: N-acetylated.1 Publication
Polyprotein: Proteolytic cleavages of the polyprotein yield mature proteins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1432 – 1433CleavageCurated2
Sitei2345 – 2346Cleavage1 Publication2

Keywords - PTMi

Acetylation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini369 – 543SF3 helicasePROSITE-ProRule annotationAdd BLAST175
Domaini1914 – 2042RdRp catalyticPROSITE-ProRule annotationAdd BLAST129

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1176 – 1179Poly-ArgSequence analysis4

Family and domain databases

InterProiView protein in InterPro
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR009003. Peptidase_S1_PA.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
PfamiView protein in Pfam
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Polyprotein (identifier: C1JCT1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEKTQTFVQ NETHVLDMTS DFKSDLSLEK VTSSVEQTDD LVSKIINNND
60 70 80 90 100
LDIKDLSFLR NLLLSTLQYL GIAKFVAINI TLSILSILML LINSCAKFTR
110 120 130 140 150
IVNLSSHILN IITTLGLYFQ VSSMEIEEIT QTFENEFGTY DDDKILSHYI
160 170 180 190 200
KICNLPNRKD VYEYISLNDL KYKIKLPDIS FYELKNDILS KNKNLHLWIF
210 220 230 240 250
QKFTDEFLAM WFGVQPYRIS NLREMLVISR QGFIPKDLFN EIRKLCNMGV
260 270 280 290 300
SVIISFIQSK LFDEPFKKRD CTQALKDASV ISSPFDTLWN LISKQVCDNS
310 320 330 340 350
AEERFTQTIL DFTSEFDNFL GIPNYKFAKN QKLVNTISKS LDACAKFIRD
360 370 380 390 400
CPKDKQTEIF PLQGLHTATV KRRNEILTNV MPKFARQEPF VVLFQGPGGI
410 420 430 440 450
GKTHLVQQLA TKCVNSFYQD HEDDYIEISP DDKYWPPLSG QRVAFFDEAG
460 470 480 490 500
NLNDLTEDLL FRNIKSICSP AYFNCAAADI EHKISPCPFE LVFATVNTDL
510 520 530 540 550
DTLQSKISST FGQASVFPIW RRCIVVECSW NEKELGPFNY KNPSGHRSDY
560 570 580 590 600
SHITMNYMSY DDKTQKLALE KEINFDTLFD MIRLRFRKKQ QEHDTKISIL
610 620 630 640 650
NNEIQRQSNS KQHFSVCLYG EPGQGKTYNL NKLITTFANA TNLKIGSEEK
660 670 680 690 700
PSIHIFDDYI KDENDENCSK FMDIYNNKLP NNSVIFSATN VYPKTHFFPT
710 720 730 740 750
FFLTNLIYAF IQPFKQVGLY RRLGFDGYTD IPNSSVNAPI FVQNFKFYER
760 770 780 790 800
KQHICYFLSL EFLKNIICYI FFFLYFPLKF IKKIDLIEIK DVNKYVYDRY
810 820 830 840 850
INFLSLSKQI EIVEYPPNLE NVEFDFRFNM NKFHRVSFNN PFELDKYIHF
860 870 880 890 900
NKNSYENLLH FDWKMYLSPR VKHRLALSYE KFFITISEVN KEIIIEELKR
910 920 930 940 950
YVLLFKQFNI DPNMEINLGE YGSFYYINGK IHLMTINIES NVSEIPVFTD
960 970 980 990 1000
GDYVYISEHK IPVIDLFDNI NINSKYNLSF DQSIALNSFK TGDSFYSNAK
1010 1020 1030 1040 1050
VRKSLSKFVL LNYQTKFKLY LKEAKDKVKN FIETPIGHLL SILLTIFVIC
1060 1070 1080 1090 1100
YASFKIYSKF SNFFSKDQAI EDQRKGEKKI KKITNYDSDG VQPQRKGEKK
1110 1120 1130 1140 1150
IKKVTNYDSD GVQPQSNVKV EEEIKLVFDP TGQKLLFGND FTSELETLVE
1160 1170 1180 1190 1200
LEKDDEEFTK SKIDNKSMAG LRREVRRRRY ARSKKAQIEK QEVLTLPDVN
1210 1220 1230 1240 1250
GFEGGKPYFQ IAEEKARKNL CQIYMIANNE NCIASKFSDH IVCYGLFVFK
1260 1270 1280 1290 1300
KRLASVGHIV EALKCAPGYN LYAGCDQFNG KLYKMNLVRN YRKRELSVWD
1310 1320 1330 1340 1350
VDCPNDFVDL TSFFIPKEEL YDAENCNTVL GRFGMNKREV YLYGNCEFIQ
1360 1370 1380 1390 1400
EFFKVDNKGA QEFGYIDWAT VDITLTTGGD CGLPYYICER KKFHNKIMGL
1410 1420 1430 1440 1450
HFAGNNVNHK TIGMSALIYK EDLVVWKGAE RQSKCKFCDV KDIIIAQPDI
1460 1470 1480 1490 1500
PKEKYKGYNH EIVWNSLHES SPTTLNEELE HYLNIFPKFT GTIIKHSGDK
1510 1520 1530 1540 1550
FYGSVKHSHT QFISKFKTEL TVTNGWKLST AGDCQFESNH ISPNTEVMYR
1560 1570 1580 1590 1600
VVDVQFNSIF KAFKSQPYIK NFRLIANVYE KDGKQRVTIL TIIPVSDFNV
1610 1620 1630 1640 1650
KQQTVRQALV PLHLNEDEEV YVTEDVSDIF KTAIKRKQRG ILPDVPYETV
1660 1670 1680 1690 1700
ENETVEILGI THRNMTPEPA QMYKPTPFYK LALKFNLDHK LPVNFNMKDC
1710 1720 1730 1740 1750
PQEQKDMMVL DRLGQPNPRI TQSLKWAHKD YSPDYELRKY VKEQYMCNIM
1760 1770 1780 1790 1800
EYYAGCNLLT EEQILKGYGP NHRLYGALGG MEIDSSIGWT MKELYRVTKK
1810 1820 1830 1840 1850
SDVINLDSNG NYSFLNNEAA QYTQELLKIS MEQAHNGQRY YTAFNELMKM
1860 1870 1880 1890 1900
EKLKPSKNFI PRTFTAQDLN GVLMERWILG EFTARALAWD ENCAVGCNPY
1910 1920 1930 1940 1950
ATFHKFATKF FKFKNFFSCD YKNFDRTIPK CVFEDFRDML IQANPHMKNE
1960 1970 1980 1990 2000
IYACFQTIID RIQVSGNSIL LVHGGMPSGC VPTAPLNSKV NDIMIYTAYV
2010 2020 2030 2040 2050
NILRRADRGD ITSYRYYRDL VCRLFYGDDV IIAVDDSIAD IFNCQTLSEE
2060 2070 2080 2090 2100
MKILFGMNMT DGSKSDIIPK FETIETLSFI SRFFRPLKHQ ENFIVGALKK
2110 2120 2130 2140 2150
ISIQTHFYYA TDDTPEHFGQ VFKTIQEEAA LWEEEYFNKI QSYIQEIIRK
2160 2170 2180 2190 2200
FPEISKFFNF ESYKSIQKRY IMNGWNEFVK LEKLDLNLNK KKSSKVTGIH
2210 2220 2230 2240 2250
SKQYSKFLKF LSRIENEKAA LEGNFNKESV NTWYFKMSKA MHLNEIFQKG
2260 2270 2280 2290 2300
LISKPLAEFY FNEGQKMWDC NITFRRSKDD LPFTFSGSGT TKACAREQAA
2310 2320 2330 2340 2350
EEALVLFSQE DEIVRQINDI QSDCKFCKKM IRYKKLLSGV SIQRQMNVSK
2360 2370 2380 2390 2400
ITENHVPSAG MMATDPSVAP DSGIATNTQT PSISRVLNPI ARALDNPAGT
2410 2420 2430 2440 2450
GAPFDKHTYV YNVFTRWPEM STVVNKSLAA GAEVFKISLD PNKLPKRILQ
2460 2470 2480 2490 2500
YIQFHKTIIP QIEVQILIGG AAGTVGWLKV GWVPDASTAK KYSLDDLQLV
2510 2520 2530 2540 2550
ASETINLNST ITMSMIINDS RRNGMFRLTK SDPEPWPGIV CLVEHPITNV
2560 2570 2580
QRNDDVNYPV IVSVRLGPDC QLMQPYNDLN
Note: Produced by conventional translation.1 Publication
Length:2,580
Mass (Da):299,117
Last modified:May 26, 2009 - v1
Checksum:i238B7E9621BEAD60
GO
Isoform Polyprotein-FSD (identifier: C1JCT2-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry C1JCT2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting.1 Publication
Length:3,390
Mass (Da):390,107
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ528584 Genomic RNA. Translation: ACO37271.1.
RefSeqiYP_002790879.1. NC_012531.1. [C1JCT1-1]

Genome annotation databases

GeneIDi7751223.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiPOL_SINV3
AccessioniPrimary (citable) accession number: C1JCT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2017
Last sequence update: May 26, 2009
Last modified: January 31, 2018
This is version 47 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome