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Protein

Mu-conotoxin BuIIIB

Gene
N/A
Organism
Conus bullatus (Bubble cone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mu-conotoxins block voltage-gated sodium channels (Nav). This synthetic toxin potently blocks rNav1.2/SCN2A, and rNav1.4/SCN4A. It also moderately blocks rNav1.1/SCN1A, rNav1.3/SCN3A, rNav1.5/SCN5, and mNav1.6/SCN8A. The block of SCN4A is very slowly reversible.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Mu-conotoxin BuIIIB
Alternative name(s):
Conotoxin Bu16
OrganismiConus bullatus (Bubble cone)
Taxonomic identifieri89438 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri3715. BuIIIB precursor.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi52 – 554Missing : 4-fold increase in blocking SCN3A (K(d) is 53 nM). 1 Publication
Mutagenesisi52 – 521V → A: 3.5-decrease in blocking SCN3A (K(d) is 710 nM). 1 Publication
Mutagenesisi53 – 531G → A: 5-fold increase in blocking SCN3A (K(d) is 36 nM). 1 Publication
Mutagenesisi53 – 531G → A: Mutated into D-Ala. 40-fold increase in blocking SCN3A (K(d) is 4.8 nM). 1 Publication
Mutagenesisi54 – 541E → A: 13-fold increase in blocking SCN3A (K(d) is 15 nM). 1 Publication
Mutagenesisi55 – 551R → A: No change in blocking SCN3A (K(d) is 118 nM). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Propeptidei23 – 5129By similarityPRO_0000384433Add
BLAST
Peptidei52 – 7524Mu-conotoxin BuIIIBPRO_0000384434Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 681 Publication
Disulfide bondi57 ↔ 741 Publication
Disulfide bondi64 ↔ 751 Publication
Modified residuei75 – 751Cysteine amideBy similarity

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom duct.

Structurei

Secondary structure

1
78
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi54 – 574Combined sources
Helixi60 – 623Combined sources
Turni63 – 653Combined sources
Helixi66 – 716Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LO9NMR-A52-75[»]
2LOCNMR-A52-75[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The cysteine framework is III (CC-C-C-CC). Classified in the M-5 branch, since 5 residues stand between the fourth and the fifth cysteine residues.

Sequence similaritiesi

Belongs to the conotoxin M superfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR004214. Conotoxin.
[Graphical view]
PfamiPF02950. Conotoxin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C1J5M6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSKLGVLLT ICLLLFPLFA LPQDGDQPAD RPAERMQDDI SSEQNPLLEK
60 70
RVGERCCKNG KRGCGRWCRD HSRCCGRR
Length:78
Mass (Da):8,877
Last modified:May 26, 2009 - v1
Checksum:i907D09C9B4DE3164
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ240166 mRNA. Translation: ACO50771.1.

Cross-referencesi

Web resourcesi

Biological Magnetic Resonance Data Bank

synthetic mu-BuIIIB

Biological Magnetic Resonance Data Bank

synthetic mutagen mu-[D-Ala2]BuIIIB

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ240166 mRNA. Translation: ACO50771.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LO9NMR-A52-75[»]
2LOCNMR-A52-75[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri3715. BuIIIB precursor.

Family and domain databases

InterProiIPR004214. Conotoxin.
[Graphical view]
PfamiPF02950. Conotoxin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCM3B_CONBU
AccessioniPrimary (citable) accession number: C1J5M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: March 4, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.