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Protein

Major histocompatibility complex class I-related gene protein

Gene

MR1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites (By similarity). Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611Pathogen-derived ligandBy similarity

GO - Molecular functioni

  1. antigen binding Source: GO_Central
  2. peptide antigen binding Source: InterPro

GO - Biological processi

  1. antigen processing and presentation Source: GO_Central
  2. antigen processing and presentation of peptide antigen via MHC class I Source: UniProtKB-KW
  3. cytokine production involved in immune response Source: Ensembl
  4. defense response to Gram-negative bacterium Source: Ensembl
  5. innate immune response Source: UniProtKB-KW
  6. interleukin-17 production Source: Ensembl
  7. interleukin-1 beta production Source: Ensembl
  8. positive regulation of T cell mediated cytotoxicity Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Major histocompatibility complex class I-related gene protein
Short name:
MHC class I-related gene protein
Alternative name(s):
Class I histocompatibility antigen-like protein
Gene namesi
Name:MR1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 16

Subcellular locationi

Cell membrane By similarity; Single-pass type I membrane protein By similarity; Extracellular side By similarity. Endoplasmic reticulum By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 298280ExtracellularSequence AnalysisAdd
BLAST
Transmembranei299 – 31921HelicalSequence AnalysisAdd
BLAST
Topological domaini320 – 33617CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. MHC class I protein complex Source: UniProtKB-KW
  4. plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, MHC I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 336318Major histocompatibility complex class I-related gene proteinPRO_0000425531Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi103 – 1031N-linked (GlcNAc...)Curated
Disulfide bondi116 ↔ 179PROSITE-ProRule annotation1 Publication
Disulfide bondi218 ↔ 274PROSITE-ProRule annotation1 Publication

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Gene expression databases

ExpressionAtlasiC1ITJ8. baseline.

Interactioni

Subunit structurei

Heterodimerizes with B2M, this interaction is required for surface expression. Associated with the peptide-loading complex, TAPBP, CALR and PDIA3.2 Publications

Structurei

Secondary structure

1
336
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi21 – 3010Combined sources
Beta strandi39 – 468Combined sources
Beta strandi49 – 5810Combined sources
Beta strandi62 – 654Combined sources
Helixi66 – 716Combined sources
Helixi74 – 10229Combined sources
Beta strandi109 – 11810Combined sources
Beta strandi124 – 1329Combined sources
Beta strandi135 – 1417Combined sources
Turni142 – 1454Combined sources
Beta strandi146 – 1516Combined sources
Helixi154 – 1629Combined sources
Turni163 – 1653Combined sources
Helixi166 – 17611Combined sources
Helixi178 – 19417Combined sources
Beta strandi201 – 2066Combined sources
Beta strandi215 – 22612Combined sources
Beta strandi229 – 2346Combined sources
Beta strandi243 – 2508Combined sources
Beta strandi252 – 2543Combined sources
Beta strandi256 – 2638Combined sources
Beta strandi272 – 2809Combined sources
Beta strandi282 – 2887Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IIQX-ray2.86C19-295[»]
4L8SX-ray2.90C19-295[»]
4L9LX-ray3.40C19-295[»]
4LCCX-ray3.26C19-295[»]
ProteinModelPortaliC1ITJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini199 – 29597Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 197179Ligand-bindingBy similarityAdd
BLAST
Regioni19 – 10587Alpha-1Add
BLAST
Regioni106 – 19792Alpha-2Add
BLAST
Regioni198 – 28992Alpha-3Add
BLAST
Regioni290 – 2989Connecting peptideBy similarity

Domaini

The alpha-3 region and to a lesser extent the transmembrane and cytosolic domains regulate surface expression (By similarity). The alpha-3 region mediates interaction with B2M.By similarity
The ligand-binding groove is ideally suited to present small organic compounds that can originate from vitamins rather than antigenic peptides.By similarity

Sequence similaritiesi

Belongs to the MHC class I family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000118960.
HOGENOMiHOG000296917.
InParanoidiC1ITJ8.
OMAiGYVDSHP.
TreeFamiTF336617.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C1ITJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLLLPLIIV LMMKLSDART HSLRYFRLGI SEPGYGIPEF ISAGYVDSHP
60 70 80 90 100
ITMYNSVSQL KEPRALWMEE NLAPDHWERY TQLLRGWQQA FKVELKQLQH
110 120 130 140 150
HYNHSGFHTY QRMIGCELLE DGSITGFLQY AYDGQDFLIF NKDTLSWMAM
160 170 180 190 200
DNVADIIRRV WEANRHELQY QKNWLEEECI AWLKRFLEYG KDALQRTEPP
210 220 230 240 250
KVRVNHKETF PGITTLYCRA YGFYPPEISI NWMKNGEEIF QDTDYGGILP
260 270 280 290 300
SGDGTYQTWV SVELDPQNGD IYSCHVEHGG VHMVLQGFQE SETILLVVKA
310 320 330
VGFIVLAIAL AGVGILAWRK RPRGKNKVIC LSTPEH
Length:336
Mass (Da):38,894
Last modified:May 26, 2009 - v1
Checksum:iD0501CC96F59A2C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU792881 mRNA. Translation: ACH56827.1.
FJ028657 mRNA. Translation: ACN18277.1.
DAAA02043522 Genomic DNA. No translation available.
DAAA02043523 Genomic DNA. No translation available.
RefSeqiNP_001177227.1. NM_001190298.1.
UniGeneiBt.63045.

Genome annotation databases

EnsembliENSBTAT00000013095; ENSBTAP00000013095; ENSBTAG00000009924.
GeneIDi506206.
KEGGibta:506206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU792881 mRNA. Translation: ACH56827.1.
FJ028657 mRNA. Translation: ACN18277.1.
DAAA02043522 Genomic DNA. No translation available.
DAAA02043523 Genomic DNA. No translation available.
RefSeqiNP_001177227.1. NM_001190298.1.
UniGeneiBt.63045.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IIQX-ray2.86C19-295[»]
4L8SX-ray2.90C19-295[»]
4L9LX-ray3.40C19-295[»]
4LCCX-ray3.26C19-295[»]
ProteinModelPortaliC1ITJ8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013095; ENSBTAP00000013095; ENSBTAG00000009924.
GeneIDi506206.
KEGGibta:506206.

Organism-specific databases

CTDi3140.

Phylogenomic databases

GeneTreeiENSGT00760000118960.
HOGENOMiHOG000296917.
InParanoidiC1ITJ8.
OMAiGYVDSHP.
TreeFamiTF336617.

Miscellaneous databases

NextBioi20867500.

Gene expression databases

ExpressionAtlasiC1ITJ8. baseline.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MR1 antigen presentation to mucosal-associated invariant T cells was highly conserved in evolution."
    Huang S., Martin E., Kim S., Yu L., Soudais C., Fremont D.H., Lantz O., Hansen T.H.
    Proc. Natl. Acad. Sci. U.S.A. 106:8290-8295(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT.
  2. "Ruminant MR1 and MAIT cells."
    Van Rhijn I.
    Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (2.86 ANGSTROMS) OF 19-295 IN COMPLEX WITH B2M AND HUMAN T-CELL RECEPTOR, DISULFIDE BONDS.

Entry informationi

Entry nameiHMR1_BOVIN
AccessioniPrimary (citable) accession number: C1ITJ8
Secondary accession number(s): F1MVL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: May 26, 2009
Last modified: February 4, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.