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C1ITJ8 (HMR1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Major histocompatibility complex class I-related gene protein

Short name=MHC class I-related gene protein
Alternative name(s):
Class I histocompatibility antigen-like protein
Gene names
Name:MR1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites By similarity. Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent. Ref.1

Subunit structure

Heterodimerizes with B2M, this interaction is required for surface expression. Associated with the peptide-loading complex, TAPBP, CALR and PDIA3. Ref.1

Subcellular location

Cell membrane; Single-pass type I membrane protein; Extracellular side By similarity. Endoplasmic reticulum By similarity.

Domain

The alpha-3 region and to a lesser extent the transmembrane and cytosolic domains regulate surface expression By similarity. The alpha-3 region mediates interaction with B2M.

The ligand-binding groove is ideally suited to present small organic compounds that can originate from vitamins rather than antigenic peptides By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the MHC class I family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processImmunity
Innate immunity
   Cellular componentCell membrane
Endoplasmic reticulum
Membrane
MHC I
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation

Inferred from Biological aspect of Ancestor. Source: RefGenome

antigen processing and presentation of peptide antigen via MHC class I

Inferred from electronic annotation. Source: UniProtKB-KW

cytokine production involved in immune response

Inferred from electronic annotation. Source: Ensembl

defense response to Gram-negative bacterium

Inferred from electronic annotation. Source: Ensembl

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

interleukin-1 beta production

Inferred from electronic annotation. Source: Ensembl

interleukin-17 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell mediated cytotoxicity

Inferred from electronic annotation. Source: InterPro

   Cellular_componentMHC class I protein complex

Inferred from electronic annotation. Source: UniProtKB-KW

endoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionantigen binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

peptide antigen binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 By similarity
Chain19 – 336318Major histocompatibility complex class I-related gene protein
PRO_0000425531

Regions

Topological domain19 – 298280Extracellular Potential
Transmembrane299 – 31921Helical; Potential
Topological domain320 – 33617Cytoplasmic Potential
Domain199 – 29597Ig-like C1-type
Region19 – 197179Ligand-binding By similarity
Region19 – 10587Alpha-1
Region106 – 19792Alpha-2
Region198 – 28992Alpha-3
Region290 – 2989Connecting peptide By similarity

Sites

Binding site611Pathogen-derived ligand By similarity

Amino acid modifications

Glycosylation1031N-linked (GlcNAc...) Probable
Disulfide bond116 ↔ 179 Ref.4
Disulfide bond218 ↔ 274 Ref.4

Secondary structure

............................................. 336
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
C1ITJ8 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: D0501CC96F59A2C1

FASTA33638,894
        10         20         30         40         50         60 
MMLLLPLIIV LMMKLSDART HSLRYFRLGI SEPGYGIPEF ISAGYVDSHP ITMYNSVSQL 

        70         80         90        100        110        120 
KEPRALWMEE NLAPDHWERY TQLLRGWQQA FKVELKQLQH HYNHSGFHTY QRMIGCELLE 

       130        140        150        160        170        180 
DGSITGFLQY AYDGQDFLIF NKDTLSWMAM DNVADIIRRV WEANRHELQY QKNWLEEECI 

       190        200        210        220        230        240 
AWLKRFLEYG KDALQRTEPP KVRVNHKETF PGITTLYCRA YGFYPPEISI NWMKNGEEIF 

       250        260        270        280        290        300 
QDTDYGGILP SGDGTYQTWV SVELDPQNGD IYSCHVEHGG VHMVLQGFQE SETILLVVKA 

       310        320        330 
VGFIVLAIAL AGVGILAWRK RPRGKNKVIC LSTPEH 

« Hide

References

« Hide 'large scale' references
[1]"MR1 antigen presentation to mucosal-associated invariant T cells was highly conserved in evolution."
Huang S., Martin E., Kim S., Yu L., Soudais C., Fremont D.H., Lantz O., Hansen T.H.
Proc. Natl. Acad. Sci. U.S.A. 106:8290-8295(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT.
[2]"Ruminant MR1 and MAIT cells."
Van Rhijn I.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"A whole-genome assembly of the domestic cow, Bos taurus."
Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D., Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G., Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.
Genome Biol. 10:R42.01-R42.10(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hereford.
[4]"The molecular basis for Mucosal-Associated Invariant T cell recognition of MR1 proteins."
Lopez-Sagaseta J., Dulberger C.L., Crooks J.E., Parks C.D., Luoma A.M., McFedries A., Van Rhijn I., Saghatelian A., Adams E.J.
Proc. Natl. Acad. Sci. U.S.A. 110:E1771-E1778(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.86 ANGSTROMS) OF 19-295 IN COMPLEX WITH B2M AND HUMAN T-CELL RECEPTOR, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU792881 mRNA. Translation: ACH56827.1.
FJ028657 mRNA. Translation: ACN18277.1.
DAAA02043522 Genomic DNA. No translation available.
DAAA02043523 Genomic DNA. No translation available.
RefSeqNP_001177227.1. NM_001190298.1.
UniGeneBt.63045.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4IIQX-ray2.86C19-295[»]
4L8SX-ray2.90C19-295[»]
4L9LX-ray3.40C19-295[»]
4LCCX-ray3.26C19-295[»]
ProteinModelPortalC1ITJ8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000013095; ENSBTAP00000013095; ENSBTAG00000009924.
GeneID506206.
KEGGbta:506206.

Organism-specific databases

CTD3140.

Phylogenomic databases

GeneTreeENSGT00750000117285.
HOGENOMHOG000296917.
OMAREANQHE.
TreeFamTF336617.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
[Graphical view]
PRINTSPR01638. MHCCLASSI.
SMARTSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20867500.

Entry information

Entry nameHMR1_BOVIN
AccessionPrimary (citable) accession number: C1ITJ8
Secondary accession number(s): F1MVL8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: May 26, 2009
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references