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Protein

Major histocompatibility complex class I-related gene protein

Gene

MR1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites (By similarity). Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61Pathogen-derived ligandBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Major histocompatibility complex class I-related gene protein
Short name:
MHC class I-related gene protein
Alternative name(s):
Class I histocompatibility antigen-like protein
Gene namesi
Name:MR1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componentsi: Chromosome 16, Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 298ExtracellularSequence analysisAdd BLAST280
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Topological domaini320 – 336CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, MHC I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000042553119 – 336Major histocompatibility complex class I-related gene proteinAdd BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi103N-linked (GlcNAc...)Curated1
Disulfide bondi116 ↔ 179PROSITE-ProRule annotation1 Publication
Disulfide bondi218 ↔ 274PROSITE-ProRule annotation1 Publication

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiC1ITJ8.
PRIDEiC1ITJ8.

Expressioni

Gene expression databases

BgeeiENSBTAG00000009924.
ExpressionAtlasiC1ITJ8. differential.

Interactioni

Subunit structurei

Heterodimerizes with B2M, this interaction is required for surface expression. Associated with the peptide-loading complex, TAPBP, CALR and PDIA3.2 Publications

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013095.

Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 30Combined sources10
Beta strandi39 – 46Combined sources8
Beta strandi49 – 58Combined sources10
Beta strandi62 – 65Combined sources4
Helixi66 – 71Combined sources6
Helixi74 – 102Combined sources29
Beta strandi109 – 118Combined sources10
Beta strandi124 – 132Combined sources9
Beta strandi135 – 141Combined sources7
Turni142 – 145Combined sources4
Beta strandi146 – 151Combined sources6
Helixi154 – 162Combined sources9
Turni163 – 165Combined sources3
Helixi166 – 176Combined sources11
Helixi178 – 194Combined sources17
Beta strandi201 – 206Combined sources6
Beta strandi215 – 226Combined sources12
Beta strandi229 – 234Combined sources6
Beta strandi243 – 250Combined sources8
Beta strandi252 – 254Combined sources3
Beta strandi256 – 263Combined sources8
Beta strandi272 – 280Combined sources9
Beta strandi282 – 288Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IIQX-ray2.86C19-295[»]
4L8SX-ray2.90C19-295[»]
4L9LX-ray3.40C19-295[»]
4LCCX-ray3.26C19-295[»]
ProteinModelPortaliC1ITJ8.
SMRiC1ITJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 295Ig-like C1-typeAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 197Ligand-bindingBy similarityAdd BLAST179
Regioni19 – 105Alpha-1Add BLAST87
Regioni106 – 197Alpha-2Add BLAST92
Regioni198 – 289Alpha-3Add BLAST92
Regioni290 – 298Connecting peptideBy similarity9

Domaini

The alpha-3 region and to a lesser extent the transmembrane and cytosolic domains regulate surface expression (By similarity). The alpha-3 region mediates interaction with B2M.By similarity
The ligand-binding groove is ideally suited to present small organic compounds that can originate from vitamins rather than antigenic peptides.By similarity

Sequence similaritiesi

Belongs to the MHC class I family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II5V. Eukaryota.
ENOG4111K8F. LUCA.
GeneTreeiENSGT00760000118960.
HOGENOMiHOG000296917.
InParanoidiC1ITJ8.
OMAiTWMKNGE.
OrthoDBiEOG091G0K1X.
TreeFamiTF336617.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C1ITJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLLLPLIIV LMMKLSDART HSLRYFRLGI SEPGYGIPEF ISAGYVDSHP
60 70 80 90 100
ITMYNSVSQL KEPRALWMEE NLAPDHWERY TQLLRGWQQA FKVELKQLQH
110 120 130 140 150
HYNHSGFHTY QRMIGCELLE DGSITGFLQY AYDGQDFLIF NKDTLSWMAM
160 170 180 190 200
DNVADIIRRV WEANRHELQY QKNWLEEECI AWLKRFLEYG KDALQRTEPP
210 220 230 240 250
KVRVNHKETF PGITTLYCRA YGFYPPEISI NWMKNGEEIF QDTDYGGILP
260 270 280 290 300
SGDGTYQTWV SVELDPQNGD IYSCHVEHGG VHMVLQGFQE SETILLVVKA
310 320 330
VGFIVLAIAL AGVGILAWRK RPRGKNKVIC LSTPEH
Length:336
Mass (Da):38,894
Last modified:May 26, 2009 - v1
Checksum:iD0501CC96F59A2C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU792881 mRNA. Translation: ACH56827.1.
FJ028657 mRNA. Translation: ACN18277.1.
DAAA02043522 Genomic DNA. No translation available.
DAAA02043523 Genomic DNA. No translation available.
RefSeqiNP_001177227.1. NM_001190298.1.
UniGeneiBt.63045.

Genome annotation databases

EnsembliENSBTAT00000013095; ENSBTAP00000013095; ENSBTAG00000009924.
GeneIDi506206.
KEGGibta:506206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU792881 mRNA. Translation: ACH56827.1.
FJ028657 mRNA. Translation: ACN18277.1.
DAAA02043522 Genomic DNA. No translation available.
DAAA02043523 Genomic DNA. No translation available.
RefSeqiNP_001177227.1. NM_001190298.1.
UniGeneiBt.63045.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IIQX-ray2.86C19-295[»]
4L8SX-ray2.90C19-295[»]
4L9LX-ray3.40C19-295[»]
4LCCX-ray3.26C19-295[»]
ProteinModelPortaliC1ITJ8.
SMRiC1ITJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013095.

Proteomic databases

PaxDbiC1ITJ8.
PRIDEiC1ITJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013095; ENSBTAP00000013095; ENSBTAG00000009924.
GeneIDi506206.
KEGGibta:506206.

Organism-specific databases

CTDi3140.

Phylogenomic databases

eggNOGiENOG410II5V. Eukaryota.
ENOG4111K8F. LUCA.
GeneTreeiENSGT00760000118960.
HOGENOMiHOG000296917.
InParanoidiC1ITJ8.
OMAiTWMKNGE.
OrthoDBiEOG091G0K1X.
TreeFamiTF336617.

Gene expression databases

BgeeiENSBTAG00000009924.
ExpressionAtlasiC1ITJ8. differential.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMR1_BOVIN
AccessioniPrimary (citable) accession number: C1ITJ8
Secondary accession number(s): F1MVL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: May 26, 2009
Last modified: November 30, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.