C1FZL3 (DAPB_PARBD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dipeptidyl-aminopeptidase B
Short name=DPAP B
EC=3.4.14.5

Gene names

Name:	DAPB
ORF Names:	PADG_00053

Organism

Paracoccidioides brasiliensis (strain Pb18) [Complete proteome]

Taxonomic identifier

502780 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Paracoccidioides › Paracoccidioides brasiliensis

Protein attributes

Sequence length	912 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline By similarity.
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein By similarity. Note: Lysosome-like vacuoles By similarity.
Sequence similarities	Belongs to the peptidase S9B family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 912	912	Probable dipeptidyl-aminopeptidase B		PRO_0000412154
Regions
	Topological domain	1 – 92	92	Cytoplasmic Potential
	Transmembrane	93 – 113	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	114 – 912	799	Vacuolar Potential
Sites
	Active site	751	1	Charge relay system By similarity
	Active site	828	1	Charge relay system By similarity
	Active site	861	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	210	1	N-linked (GlcNAc...) Potential
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
	Glycosylation	569	1	N-linked (GlcNAc...) Potential
	Glycosylation	656	1	N-linked (GlcNAc...) Potential
	Glycosylation	810	1	N-linked (GlcNAc...) Potential
	Glycosylation	897	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	C1FZL3 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 7BF8C0744459EAA0	FASTA	912	102,308
	10 20 30 40 50 60 MAAEKGGSSD EERKPLTRGS MEYRDSSNSL HYSSSAASLS LAVIDRINGS THDTGPDEIG 70 80 90 100 110 120 RGDRDYSDDG EYDLEEADYI PSGGKPVQKK VKIVLGFLLF LCLSGWSLAF VLFLFGGHES 130 140 150 160 170 180 SKTSIVYEDN ISDTGSQGIK ITLDEVFDGT WSPNSRDISW IPGPNGEDGL LLEKGASISN 190 200 210 220 230 240 GYLRVEDIVS RKDPKSSKKP IVLMQKAYFN VSGEAVFPSR VWPSPDLKTV LVLSNEEKNW 250 260 270 280 290 300 RHSFTGKYWL FDVESQTGQP LDPAAKDQRV QLASWSPRSD AVVFTRDNNM FLRKLSSNEV 310 320 330 340 350 360 IKITTNGGVN LFYGVPDWVY EEEVFSGNSV TWWADDGEYI AFLRTNESSV PEYPVQYFVS 370 380 390 400 410 420 RPNGEIPKPG GESYPETRKI KYPKAGAPNP IVDLQFFDVG KDEVFSVDIK GDFADSNRLI 430 440 450 460 470 480 IEVVWASNGK VIVRSTNRES DVLHVAVIDV LSRTGKIVRK EDINALDGGW VEPSQTTRFI 490 500 510 520 530 540 PADPDNGRLN DGYIDTVIYE GRDQLAYYTP IDNPKPIVLT NGHSEVVQAP SGVDLKRGLV 550 560 570 580 590 600 YFVVAGNEPW ERHIYSVNFD GTSLQPVTNV SESSYYDVSF SNGAGYAFLK YAGPQVPWQK 610 620 630 640 650 660 VISTPANEVT FEETIEENNH LSERLRQYTL ESKIYQYIDI DGFSLPVLER RPPNFNQTKK 670 680 690 700 710 720 YPVLFYLYGG PGSQTVKKKF NVDFQSYVAA NLGYIVVTVD GRGTGFIGRK ARCIIRGNLG 730 740 750 760 770 780 HFESLDQIQA AKIWAAKPYV DESRISIWGW SYGGFMALKT IEQDGGRTFK YGIAVAPVTD 790 800 810 820 830 840 WRYYDSIYTE RYMHTPQRNP GGYDNAAISN TTALANNIRF LVMHGTADDN VHIQNSLTFI 850 860 870 880 890 900 DKLDVNNVHN YDVHFFPDSD HSIYFHNAHK IVYSRLADWL VNAFNGEWLK TYDPTPNDSI 910 LRRAATWVGM SI

References

[1]

"Annotation of Paracoccidioides brasiliensis Pb18." McEwen J.G., Puccia R., Goldman G.H., Felipe M.S., Ma L.-J.J., Henn M.R., Champion M., Cuomo C., Jaffe D.B., Young S.K., Gnerre S., Berlin A., Heiman D., Hepburn T., Sykes S., Yandava C., Alvarado L., Kodira C.D. , Guigo R., Borodovsky M., Goldman B., Klein B., Mendoza L., Nino-Vega G., San-Blas G., Sil A., de Almeida Soares C.M., Taylor J., Lander E.S., Nusbaum C., Galagan J.E., Birren B.W. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pb18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS572750 Genomic DNA. Translation: EEH43764.1.
3D structure databases
ProteinModelPortal	C1FZL3.
ModBase	Search...
Protein family/group databases
MEROPS	S09.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002471. Pept_S9_AS. IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_PARBD

Accession

Primary (citable) accession number: C1FZL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	May 26, 2009
Last modified:	June 13, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program