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C1FSH8

- TRMB_CLOBJ

UniProt

C1FSH8 - TRMB_CLOBJ

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Clostridium botulinum (strain Kyoto / Type A2)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431S-adenosyl-L-methionineUniRule annotation
Binding sitei68 – 681S-adenosyl-L-methionineUniRule annotation
Binding sitei101 – 1011S-adenosyl-L-methionineUniRule annotation
Binding sitei123 – 1231S-adenosyl-L-methionineUniRule annotation
Binding sitei127 – 1271SubstrateUniRule annotation
Binding sitei159 – 1591SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT536232:GCO3-567-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:CLM_0591
OrganismiClostridium botulinum (strain Kyoto / Type A2)
Taxonomic identifieri536232 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001374: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferasePRO_1000149651Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi536232.CLM_0591.

Structurei

3D structure databases

ProteinModelPortaliC1FSH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1346Interaction with RNAUniRule annotation
Regioni196 – 1994Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1FSH8 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLRKKWWAR PEIEASDKFA EEPKELRGKW NKEFNNNNDI HLELGCGRGG
60 70 80 90 100
FISQLVEKNK DINYVGIDLK DEVIVYAIRK VKEKEEEVKR EFKNIKFVTM
110 120 130 140 150
NIMGIAEVFD KNEISKIYIN FCNPWPKERH NKRRLTHTKL LTEYKKFLKP
160 170 180 190 200
NTEIWFKTDD KELFEDSQEY FKESGFNIEY ITYDLHNSDF KENIKTEYET
210
KFETMGMKIM FLKARLL
Length:217
Mass (Da):26,259
Last modified:May 26, 2009 - v1
Checksum:iC9CEDFA575906AD2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001581 Genomic DNA. Translation: ACO85457.1.
RefSeqiYP_002802833.1. NC_012563.1.

Genome annotation databases

EnsemblBacteriaiACO85457; ACO85457; CLM_0591.
GeneIDi7766579.
KEGGicby:CLM_0591.
PATRICi19377795. VBICloBot91161_0523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001581 Genomic DNA. Translation: ACO85457.1 .
RefSeqi YP_002802833.1. NC_012563.1.

3D structure databases

ProteinModelPortali C1FSH8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 536232.CLM_0591.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO85457 ; ACO85457 ; CLM_0591 .
GeneIDi 7766579.
KEGGi cby:CLM_0591.
PATRICi 19377795. VBICloBot91161_0523.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci CBOT536232:GCO3-567-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Clostridium botulinum A2 Kyoto."
    Shrivastava S., Brinkac L.M., Brown J.L., Bruce D., Detter C.C., Johnson E.A., Munk C.A., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Kyoto / Type A2.

Entry informationi

Entry nameiTRMB_CLOBJ
AccessioniPrimary (citable) accession number: C1FSH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: October 1, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3