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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Clostridium botulinum (strain Kyoto / Type A2)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotationSAAS annotation

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotationSAAS annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi13Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi156Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi160Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi163Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Ligand4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCBOT536232:G1GVA-2581-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimOUniRule annotationSAAS annotation (EC:2.8.4.4UniRule annotationSAAS annotation)
Short name:
S12 MTTaseUniRule annotation
Short name:
S12 methylthiotransferaseUniRule annotation
Alternative name(s):
Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation
Ribosome maturation factor RimOUniRule annotation
Gene namesi
Name:rimOUniRule annotation
Ordered Locus Names:CLM_2699Imported
OrganismiClostridium botulinum (strain Kyoto / Type A2)Imported
Taxonomic identifieri536232 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001374 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliC1FS47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 119MTTase N-terminalInterPro annotationAdd BLAST116
Domaini375 – 441TRAMInterPro annotationAdd BLAST67

Sequence similaritiesi

Belongs to the methylthiotransferase family. RimO subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000224766
KOiK14441
OMAiCLSERYK

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01865 MTTase_RimO, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR005840 Ribosomal_S12_MeSTrfase_RimO
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PANTHERiPTHR43837 PTHR43837, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00274 ribosomal_protein_S12_methylth, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
TIGR01125 TIGR01125, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

C1FS47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKIKVALVS LGCDKNRIDS ELMLYKLNEE AELVKNPKEA QVIIVNTCGF
60 70 80 90 100
IETAKEESIN TILQMASYKK THNCKVLVVT GCLTQRYKGE LKELIPEMDI
110 120 130 140 150
MLGVNDYDKL LESIKVFLKS GEKSFYHKYS DTKINEGNRI LTTPTYTAYV
160 170 180 190 200
RIAEGCNNFC TYCAIPRIRG KYRSRKKENI LKEVENLAKQ GVKEIILIAQ
210 220 230 240 250
DTTMYGIDIY GKKVLHELLR DISKVEGVKW IRLLYCYPEE ITKELIEEIK
260 270 280 290 300
NNDKVCKYLD LPIQQISNSV LKRMGRKTTK ETIINIIKKL RKEIEGITLR
310 320 330 340 350
TSLIVGFPGE TEGEFSELKE FVSDVKLDKL GVFKYSKEEG TSAALMKEQI
360 370 380 390 400
DEEIKEKREE EIMILQQSIS KDINKEKIGK IYEVIVEGTK EDMYYGRNYE
410 420 430 440
MSPEIDGEIY FEKDENVKIG DIIKVKVTHS LEYDLIGVVY NELSK
Length:445
Mass (Da):51,361
Last modified:May 26, 2009 - v1
Checksum:iF3A5018A43591715
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001581 Genomic DNA Translation: ACO86397.1
RefSeqiWP_004440536.1, NC_012563.1

Genome annotation databases

EnsemblBacteriaiACO86397; ACO86397; CLM_2699
KEGGicby:CLM_2699

Similar proteinsi

Entry informationi

Entry nameiC1FS47_CLOBJ
AccessioniPrimary (citable) accession number: C1FS47
Entry historyiIntegrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health