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C1FQU5

- C1FQU5_CLOBJ

UniProt

C1FQU5 - C1FQU5_CLOBJ

Protein

Endonuclease III

Gene

nth

Organism
Clostridium botulinum (strain Kyoto / Type A2)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity.UniRule annotation
    DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi187 – 1871Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi194 – 1941Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi197 – 1971Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi203 – 2031Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
    3. DNA binding Source: UniProtKB-HAMAP
    4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. base-excision repair Source: InterPro

    Keywords - Molecular functioni

    EndonucleaseImported, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repairUniRule annotation

    Keywords - Ligandi

    4Fe-4SUniRule annotation, DNA-bindingUniRule annotation, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciCBOT536232:GCO3-245-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
    Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
    Gene namesi
    Name:nthUniRule annotationImported
    Ordered Locus Names:CLM_0259Imported
    OrganismiClostridium botulinum (strain Kyoto / Type A2)Imported
    Taxonomic identifieri536232 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001374: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi536232.CLM_0259.

    Structurei

    3D structure databases

    ProteinModelPortaliC1FQU5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini100 – 12728HhHUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nth/MutY family.UniRule annotation
    Contains 1 HhH domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0177.
    HOGENOMiHOG000252208.
    KOiK10773.
    OMAiWIKLHRS.
    OrthoDBiEOG6H4KC5.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_00942. Nth.
    InterProiIPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view]
    PfamiPF10576. EndIII_4Fe-2S. 1 hit.
    PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001435. Nth. 1 hit.
    SMARTiSM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    TIGRFAMsiTIGR01083. nth. 1 hit.
    PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
    PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C1FQU5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNHEIKNVI DILVDTYPDA NCELEHRNPF ELLIATVLSA QTTDKKVNEI    50
    TKELFKEYST PKDFLKLTRE ELEEKIKKIG LYRNKSKNIL LLCKELEEKF 100
    GSQVPNDFND LTSLPGVGRK TANVVLANAF KVPTIAVDTH VFRVSNRIGL 150
    VDASNVLKTE EQLQQAIPKE LWILMHHVLI FHGRRCCVAR KPKCEECTIK 200
    KYCKYYNEEI KPS 213
    Length:213
    Mass (Da):24,574
    Last modified:May 26, 2009 - v1
    Checksum:iFC371696A57984C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001581 Genomic DNA. Translation: ACO87117.1.
    RefSeqiYP_002802521.1. NC_012563.1.

    Genome annotation databases

    EnsemblBacteriaiACO87117; ACO87117; CLM_0259.
    GeneIDi7764409.
    KEGGicby:CLM_0259.
    PATRICi19377137. VBICloBot91161_0201.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001581 Genomic DNA. Translation: ACO87117.1 .
    RefSeqi YP_002802521.1. NC_012563.1.

    3D structure databases

    ProteinModelPortali C1FQU5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 536232.CLM_0259.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACO87117 ; ACO87117 ; CLM_0259 .
    GeneIDi 7764409.
    KEGGi cby:CLM_0259.
    PATRICi 19377137. VBICloBot91161_0201.

    Phylogenomic databases

    eggNOGi COG0177.
    HOGENOMi HOG000252208.
    KOi K10773.
    OMAi WIKLHRS.
    OrthoDBi EOG6H4KC5.

    Enzyme and pathway databases

    BioCyci CBOT536232:GCO3-245-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_00942. Nth.
    InterProi IPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view ]
    Pfami PF10576. EndIII_4Fe-2S. 1 hit.
    PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001435. Nth. 1 hit.
    SMARTi SM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    TIGRFAMsi TIGR01083. nth. 1 hit.
    PROSITEi PS00764. ENDONUCLEASE_III_1. 1 hit.
    PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Clostridium botulinum A2 Kyoto."
      Shrivastava S., Brinkac L.M., Brown J.L., Bruce D., Detter C.C., Johnson E.A., Munk C.A., Smith L.A., Smith T.J., Sutton G., Brettin T.S.
      Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Kyoto / Type A2Imported.

    Entry informationi

    Entry nameiC1FQU5_CLOBJ
    AccessioniPrimary (citable) accession number: C1FQU5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 26, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3