C1F960 (KATG_ACIC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	ACP_0213

Organism

Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670) [Complete proteome] [HAMAP]

Taxonomic identifier

240015 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Acidobacteriales › Acidobacteriaceae › Acidobacterium

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Chain	16 – 732	717	Catalase-peroxidase HAMAP MF_01961		PRO_1000189068
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	260	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 219		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245) By similarity
	Cross-link	219 ↔ 245		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1F960 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: A637A407753A6F8C	FASTA	732	80,123
	10 20 30 40 50 60 MSTESKCPFA GGAYANTEAG AKGNRDWWPK QLNLDILHQH SSLSNPLEEN FDYASEFQSL 70 80 90 100 110 120 DLDAVVKDLH ALMTTSQDWW PADFGHYGGL FIRMAWHSAG TYRIYDGRGG ASRGAQRFAP 130 140 150 160 170 180 LNSWPDNVNL DKARRLLWPI KQKYGRKISW ADLMILAGNV ALESMGFKTF GFGGGREDIW 190 200 210 220 230 240 EPEKDINWGP ETTWLGDERY SGNRELANPL GAVQMGLIYV NPEGPNGNPD PLASARDIRE 250 260 270 280 290 300 TFARMAMNDE ETVALIAGGH TFGKTHGAAP ASHVGLEPEG ASIEEQGLGW KNSFGTGVGK 310 320 330 340 350 360 DAITSGLEVI WTPTPTQWDM SYLETLYGYE WELTKSPAGA HQWKPKGDAG AGTVPDAFDA 370 380 390 400 410 420 KKRHQPSMLT SDIALRTDPA YDKICRDFMA HPDKYADAFA RAWYKLTHRD MGPITRYLGP 430 440 450 460 470 480 LVPKEELIWQ DPVPAVDHEL VNDADVEALK AEVLASGLTI PQLVSTAWAA ASSFRGSDRR 490 500 510 520 530 540 GGANGARIRL EPQKNWEANQ PAELAKVLAK LEGIQQKFNG AQQGGKKISL ADLIVLAGNA 550 560 570 580 590 600 GVEAAAKKAG HDVKVPFAPG RTDATQEQTD APTFAPLEPK ADGFRNYAKK GLEDAAAYLL 610 620 630 640 650 660 IDKAQLLTLT APEMTVLVGG LRALGANYGQ SKHGVFTSRP ETLTNDFFVN LLDMNTAWAK 670 680 690 700 710 720 SEKAAGEFEG RDRKTGQIKW TASIVDLVFG SNSQLRALAE VYATSDSTEA FVKDFVAAWT 730 KVMNLDRFDL KK

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51196 / DSM 11244 / JCM 7670.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001472 Genomic DNA. Translation: ACO32804.1.
RefSeq	YP_002753357.1. NC_012483.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C1F960.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7699672.
GenomeReviews	Gene locus ACP_0213 in contig CP001472_GR.
KEGG	aca:ACP_0213.
PATRIC	20663821. VBIAciCap40988_0211.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ACIC5

Accession

Primary (citable) accession number: C1F960

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program