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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi158Zinc 1UniRule annotation1
Metal bindingi161Zinc 1UniRule annotation1
Metal bindingi175Zinc 2UniRule annotation1
Metal bindingi178Zinc 2UniRule annotation1
Metal bindingi197Zinc 2UniRule annotation1
Metal bindingi200Zinc 2UniRule annotation1
Metal bindingi211Zinc 1UniRule annotation1
Metal bindingi214Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri145 – 223CR-typePROSITE-ProRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperoneUniRule annotationSAAS annotation
Biological processDNA replicationUniRule annotationSAAS annotation, Stress responseUniRule annotationSAAS annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotationImported
Ordered Locus Names:ACP_2088Imported
OrganismiAcidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)Imported
Taxonomic identifieri240015 [NCBI]
Taxonomic lineageiBacteriaAcidobacteriaAcidobacterialesAcidobacteriaceaeAcidobacterium
Proteomesi
  • UP000002207 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi240015.ACP_2088.

Structurei

3D structure databases

ProteinModelPortaliC1F925.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 76JInterPro annotationAdd BLAST66
Domaini145 – 223CR-typeInterPro annotationAdd BLAST79
Repeati158 – 165CXXCXGXG motifUniRule annotation8
Repeati175 – 182CXXCXGXG motifUniRule annotation8
Repeati197 – 204CXXCXGXG motifUniRule annotation8
Repeati211 – 218CXXCXGXG motifUniRule annotation8

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotationSAAS annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri145 – 223CR-typePROSITE-ProRule annotationAdd BLAST79

Keywords - Domaini

RepeatUniRule annotationSAAS annotation, Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.
OrthoDBiPOG091H00PT.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR036869. DnaJ_dom_sf.
IPR001623. DnaJ_domain.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR036410. HSP_DnaJ_Cys-rich_dom_sf.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

C1F925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRTANVTKL DYYEVLGVER TANDQELKTA YRKLALQYHP DRNPGNPEAE
60 70 80 90 100
EQFKACSEAY QVLSDPQKRA AYDRFGHAGV NGGGPAAGGF DGSPFGGFED
110 120 130 140 150
LGDIFGDLFG FNVGGRGSRG GNSRVQKGRD VRCDLTLAFE EAVFGKETHV
160 170 180 190 200
TIHRREACGD CNGTGAAAGR GPVTCGQCQG RGQVRYQQGF FSIARTCSAC
210 220 230 240 250
GGSGQVISDP CPTCRGDGRV DKQRNIAVHV PAGVEDGTRI RYQGEGDVGR
260 270 280 290 300
FGGPAGDLYV VLSVQPHKFF ERDGNDLHCA IPISFPQASL GTELTLPSLD
310 320 330 340 350
GEVRLKIPEG TQSGKEFRVR GKGVPHLNEH GRGDLIVQIV VQTPTKLSKV
360 370 380
QKELMRQLSD SLTVENTPTS RSSLFEKVKE IFS
Length:383
Mass (Da):41,329
Last modified:May 26, 2009 - v1
Checksum:iA828C8CE8681DE59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001472 Genomic DNA. Translation: ACO33779.1.
RefSeqiWP_015897189.1. NC_012483.1.

Genome annotation databases

EnsemblBacteriaiACO33779; ACO33779; ACP_2088.
KEGGiaca:ACP_2088.

Similar proteinsi

Entry informationi

Entry nameiC1F925_ACIC5
AccessioniPrimary (citable) accession number: C1F925
Entry historyiIntegrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: October 25, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported