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C1F6V0 (C1F6V0_ACIC5) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL ACO32840.1
Ordered Locus Names:ACP_3419
OrganismAcidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670) [Complete proteome] [HAMAP]
Taxonomic identifier240015 [NCBI]
Taxonomic lineageBacteria › Acidobacteria › Acidobacteriales › Acidobacteriaceae › Acidobacterium

Protein attributes

Sequence length878 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
C1F6V0 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 9F33423879F0AB62

FASTA87898,841
        10         20         30         40         50         60 
MSKSLVIVES PAKAKTINKY LGKDFQVEAS IGHIKDLPKN KLGVDVEKGT FEPELIVIPG 

        70         80         90        100        110        120 
KEKVVDKLRK LATKVDTIYL APDPDREGEA IAAHLEDELK AVVKKPEAIR RITFNEITPK 

       130        140        150        160        170        180 
AVTAALDHAR SVDRNLVDAQ QTRRVLDRIV GYQISPLLWD KVRRGLSAGR VQTVALRLIV 

       190        200        210        220        230        240 
EREQQIKAFK PVEYWTIDAV LKTGGNPQEF TAHFIGIDGE RARVETVDAP ALPDQKLTDE 

       250        260        270        280        290        300 
VVAQLHKADW SVREVERKER KRNAPAPFTT SKLQQDASRQ LGFNVKRTMG VAQRLYEGIE 

       310        320        330        340        350        360 
IGGDAVGLIT YMRTDSTRVA PEAIEAVRGY IQKKVGKDYL PAAPNAFKSK KDAQDAHEAI 

       370        380        390        400        410        420 
RPADPNLHPD DIRKYLSDEQ YKLYRLIWRR FVASQMMPAI FDQTTVEIAA RADRRYDFRV 

       430        440        450        460        470        480 
TGSVMKFDGF LRVYEEVKEK KDADDEALAN KLPALNNGDR LALSALKPEQ HYTEPPPRYN 

       490        500        510        520        530        540 
EASLVKELEE RGIGRPSTYA TIINTIQDRE YVQKMGGRTG RFIPTEIGTV VTGLLVKNFP 

       550        560        570        580        590        600 
YIFDTKYTAR LEEELDDIED GKERWTDLLN GFYDHFEDEL KDAATNMEDI KRMEKPTDQK 

       610        620        630        640        650        660 
CDICGSPLVL KWGKFGSFYA CSAYDKKKKD GCTFTKENYE AKPNLAAPSD EAEQEEEYCE 

       670        680        690        700        710        720 
NCGRVMVLRN GPWGPFMACP GYNEDPPCKT VRRLNQKQQQ KPPQPLDEKC PKCGEQLVLR 

       730        740        750        760        770        780 
HGQYGEFISC SGYPKCKYIK QNTIGMKCPQ CKVGEIVEKK ARRGNFFYGC SEYPKCDFTA 

       790        800        810        820        830        840 
NYKPVDRKCP ECGSAYLMEK TLKSGVYLVC PNNKKPSAEE ETTTKRKKKG DTGEGVSCSF 

       850        860        870 
TERIGDAPSE APAATATKPA AKPTAKTHGP VVEELAKV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001472 Genomic DNA. Translation: ACO32840.1.
RefSeqYP_002756414.1. NC_012483.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGC1F6V0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7699536.
GenomeReviewsGene locus ACP_3419 in contig CP001472_GR.
KEGGaca:ACP_3419.
PATRIC20670039. VBIAciCap40988_3275.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAQELDETC.
ProtClustDBPRK07561.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 4 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC1F6V0_ACIC5
AccessionPrimary (citable) accession number: C1F6V0
Entry history
Integrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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