C1F5Y1 (C1F5Y1_ACIC5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485

Gene names

Name:	purF EMBL ACO32833.1
Ordered Locus Names:	ACP_1390

Organism

Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670) [Complete proteome] [HAMAP]

Taxonomic identifier

240015 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Acidobacteriales › Acidobacteriaceae › Acidobacterium

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3 Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Ontologies

Keywords
Biological process	Purine biosynthesis PIRNR PIRNR000485
Ligand	Iron Iron-sulfur PIRSR PIRSR000485-3 Magnesium PIRNR PIRNR000485 Metal-binding
Molecular function	Glycosyltransferase PIRNR PIRNR000485 EMBL ACO32833.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	62	1	For GATase activity By similarity PIRSR PIRSR000485-1
	Metal binding	300	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	446	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	498	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	501	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

	Sequence		Length	Mass (Da)
	C1F5Y1 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 9932B9B1A7C5CB50	FASTA	523	57,346
	10 20 30 40 50 60 MSRIKAEGIE SELDTPMRRI VFEGFESKIL QTLASRTKRN GGEAAPARTA SPEEDPKLRE 70 80 90 100 110 120 HCGVAAVYHH PDAARQVYLS LYALQHRGQE SAGIASADGN SISNIKGMGL VSEIFTDEVL 130 140 150 160 170 180 SKLKGDMAIG HTRYSTTGDS ALLNAQPIRV ESTKGLIAIA HNGNLVNLGN LRVELERQGA 190 200 210 220 230 240 TFQTTSDSEI IIQLIAHSTA TTLVDAIADS LRQVEGAFSI VMMTRDRIFA ARDRHGFRPL 250 260 270 280 290 300 SMGRIQNPDG PDTIVFASET CAFDLLHAKF ERDVAPGELV MVTEDGVTSR QYAEPNQSSC 310 320 330 340 350 360 IFEHVYFARP DSKIFNRWVQ DSREQMGRQL ARESHVPADL VVPVPDSGVT AAIGYAAESG 370 380 390 400 410 420 VPFRFGLIRN HYVGRTFIEP EQKVRDFGVR LKLNPVRNLL EGKRIILIDD SIIRGTTSRK 430 440 450 460 470 480 IVRMVRGAGA KEVHLRISCP PTISPCFYGV DTPRKSELIA ANQSIEEICS FIEADSLAYL 490 500 510 520 SLDGLQHACD GGEGNQYCVA CYTGNYPTAW VDVEDILPAT ATL

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001472 Genomic DNA. Translation: ACO32833.1.
RefSeq	YP_002754476.1. NC_012483.1.
3D structure databases
ProteinModelPortal	C1F5Y1.
ModBase	Search...
Protein-protein interaction databases
STRING	C1F5Y1.
Protein family/group databases
MEROPS	C44.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7699311.
GenomeReviews	Gene locus ACP_1390 in contig CP001472_GR.
KEGG	aca:ACP_1390.
PATRIC	20666091. VBIAciCap40988_1335.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VWAPGEE.
ProtClustDB	CLSK775230.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
KO	K00764.
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. PurF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C1F5Y1_ACIC5

Accession

Primary (citable) accession number: C1F5Y1

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)