C1F486 (C1F486_ACIC5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344
EC=6.3.5.2 HAMAP MF_00344

Alternative name(s):
GMP synthetase HAMAP MF_00344
Glutamine amidotransferase HAMAP MF_00344

Gene names

Name:	guaA HAMAP MF_00344 EMBL ACO32873.1
Ordered Locus Names:	ACP_1114

Organism

Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670) [Complete proteome] [HAMAP]

Taxonomic identifier

240015 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Acidobacteriales › Acidobacteriaceae › Acidobacterium

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 GMP-binding domain. HAMAP MF_00344 Contains 1 glutamine amidotransferase type-1 domain. HAMAP MF_00344

Ontologies

Keywords
Biological process	GMP biosynthesis HAMAP MF_00344 Purine biosynthesis
Domain	Glutamine amidotransferase HAMAP MF_00344
Ligand	ATP-binding HAMAP MF_00344 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00344 EMBL ACO32873.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	5 – 195	191	Glutamine amidotransferase type-1 By similarity HAMAP MF_00344
	Domain	227 – 392	166	GMP-binding By similarity HAMAP MF_00344
	Nucleotide binding	223 – 229	7	ATP By similarity HAMAP MF_00344
Sites
	Active site	82	1	Nucleophile By similarity HAMAP MF_00344
	Active site	169	1	By similarity HAMAP MF_00344
	Active site	171	1	By similarity HAMAP MF_00344

Sequences

	Sequence		Length	Mass (Da)
	C1F486 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: D669B052E1858281	FASTA	516	56,574
	10 20 30 40 50 60 METSSIVILD FGSQYTQLIA RRIREQNVFS VVLPCTASLE EVQAQRPLGI ILSGGPCSVY 70 80 90 100 110 120 DADAPPADPR VLELGLPVLG ICYGLQFITH HLGGKVRSAE KREYGHAEVM VEDAGNALFT 130 140 150 160 170 180 NVPPSVNVWM SHGDEALELP PGFHRTAKTA NAVAGIANPE RRIWAVQFHP EVKHTAHGTD 190 200 210 220 230 240 LLRNFVFNIC DAKPEWTPEL FIQSTVAAIR EKVGDGQAIC ALSGGVDSSV AAVLVHRAIG 250 260 270 280 290 300 DQLTCVFVDN GVLRLNEFEK VQQNLRGKLG LNLVAVDASK RFLDKLAGVT DPEAKRKIIG 310 320 330 340 350 360 NEFIAVFDDE AHRIAQQTGG VDWLVQGTLY PDVIESSSVK GPSQTIKSHH NVGGLPEDMK 370 380 390 400 410 420 LKLIEPLRDL FKDEVRRIGA DLGMPQDILQ RQPFPGPGLA VRILGEITPE RVALLQEADD 430 440 450 460 470 480 IVVDEIKKAG LYQQIWQSFA VLLPVKSVGV MGDQRTYAYT CAIRAVHSDD GMTADWVPLP 490 500 510 YDVLKKISSR IVNEVRGINR VVYDITSKPP GTIEWE

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001472 Genomic DNA. Translation: ACO32873.1.
RefSeq	YP_002754219.1. NC_012483.1.
3D structure databases
ProteinModelPortal	C1F486.
ModBase	Search...
Protein-protein interaction databases
STRING	C1F486.
Protein family/group databases
MEROPS	C26.A17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7699814.
GenomeReviews	Gene locus ACP_1114 in contig CP001472_GR.
KEGG	aca:ACP_1114.
PATRIC	20665583. VBIAciCap40988_1083.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C1F486_ACIC5

Accession

Primary (citable) accession number: C1F486

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)