C1F460 (MURD_ACIC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	ACP_1088

Organism

Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670) [Complete proteome] [HAMAP]

Taxonomic identifier

240015 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fibrobacteres/Acidobacteria group › Acidobacteria › Acidobacteriia › Acidobacteriales › Acidobacteriaceae › Acidobacterium › Acidobacterium capsulatum

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP-Rule MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP-Rule MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00639
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP-Rule MF_00639		PRO_1000147389
Regions
	Nucleotide binding	115 – 121	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	C1F460 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: CE61CBD6F69A8763	FASTA	461	49,076
	10 20 30 40 50 60 MELKGKKVLV VGLGKSGLAA ALFLRRRGAQ VTVSDIRSAE ALSKDIPALI EQGIAVEAGG 70 80 90 100 110 120 HGLLTFRRQD LIVVSPGVPL DTPELVQVRK FGLPIIGEVE LAARFLKGKT LAITGSNGKT 130 140 150 160 170 180 TTTSLCGAIL ERAHQHVQVG GNIGLPVIAL VDDSRDDGWS VLEISSFQLE TTERFRPGIA 190 200 210 220 230 240 VILNITPDHL DRHGSFENYV AAKERIFAAQ THDDALILNA DDDAASRAAA RASSRIFWFS 250 260 270 280 290 300 RNRVIRQGAF VHEGNILFRA AEDAATEPIL PLSEIPLKGA HNVENVLAAV CAARLAGVSA 310 320 330 340 350 360 EAIRDAVRDF RAVEHRLEFV AEIGGVSFYN DSKATNVDAA RKAIEAFPGG IHLILGGKDK 370 380 390 400 410 420 NSDYRTLRPL MAGRVKAVYT IGSAAEKIMT HLDGAVPLIA AGTLDLAVNL AGGAASPGDV 430 440 450 460 VLLAPACSSF DQFENYEQRG QVFKDLVLAH RGAAAWQNAS A

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51196 / DSM 11244 / JCM 7670.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001472 Genomic DNA. Translation: ACO34542.1.
RefSeq	YP_002754193.1. NC_012483.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	240015.ACP_1088.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACO34542; ACO34542; ACP_1088.
GeneID	7700691.
KEGG	aca:ACP_1088.
PATRIC	20665529. VBIAciCap40988_1056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HOG000049428.
KO	K01925.
OMA	RCGELDV.
ProtClustDB	CLSK775010.
Enzyme and pathway databases
BioCyc	ACAP240015:GKF4-1066-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.40.50.720. 1 hit. 3.90.190.20. 1 hit.
HAMAP	MF_00639. MurD.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_ACIC5

Accession

Primary (citable) accession number: C1F460

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	May 29, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program