C1F3P3 (SYP_ACIC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	ACP_2832

Organism

Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670) [Complete proteome] [HAMAP]

Taxonomic identifier

240015 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Acidobacteriales › Acidobacteriaceae › Acidobacterium

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Proline--tRNA ligase HAMAP MF_01569		PRO_1000185481

Sequences

	Sequence		Length	Mass (Da)
	C1F3P3 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 90844F150B4E2AC1	FASTA	585	64,330
	10 20 30 40 50 60 MHRWSRLFVP TLREAPSDAE VASHKFLLRS GYIRQLGAGI YSYLLFGQRS INKIVGIVRE 70 80 90 100 110 120 EMDKIGQEFL LPAIHPADLW RESGRWTVMG DNMFRLKDRK GAELCLGMTH EEVMTDIARN 130 140 150 160 170 180 ELRSYKQLPQ IWYQIQNKFR DEPRPRSGLL RVRQFLMKDS YSFDLNQAGL DKSYDLHDAA 190 200 210 220 230 240 YRAIFTRCGL NFVVVEADSG AMGGSGSQEF MVYTDAGEDL IASCAKCGYA ANMEKATSKL 250 260 270 280 290 300 APVEELAATG DGQPELISTP GQAAIADICK FLGMQPHSDI KCVAYMAEVP SEDGKTSTWE 310 320 330 340 350 360 PVAAFLRGDH FVNETKLLAV VGATELRPMQ AEELEKWMHG PAGYLGPVGL TPAKKLRDGS 370 380 390 400 410 420 LNVVLDLGLE GRRNLVAGAN KLDYHYRNVT PGRDFTWTVA ADIRNAAEGE GCPVCGEPLK 430 440 450 460 470 480 VAKAVEIGHI FKLGTKYTES MGARVLDENG KEVMPVMGCY GIGIERILTA SIEQNNDANG 490 500 510 520 530 540 FWLPRSIAPF DVVVTITNMA DETLRQAGEA LAAQLEAAGY DVLLDDREER AGVKFKDADL 550 560 570 580 VGIPYRFNVG KKTLEGKTEL VTRATASSQD VALDAAVEAL KALTA

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51196 / DSM 11244 / JCM 7670.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001472 Genomic DNA. Translation: ACO33322.1.
RefSeq	YP_002755848.1. NC_012483.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C1F3P3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7700810.
GenomeReviews	Gene locus ACP_2832 in contig CP001472_GR.
KEGG	aca:ACP_2832.
PATRIC	20668905. VBIAciCap40988_2716.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IQPAELW.
ProtClustDB	PRK09194.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ACIC5

Accession

Primary (citable) accession number: C1F3P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program