C1F3C4 (DXS_ACIC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	ACP_2818

Organism

Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670) [Complete proteome] [HAMAP]

Taxonomic identifier

240015 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Acidobacteriales › Acidobacteriaceae › Acidobacterium

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 627	627	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000132915

Sequences

	Sequence		Length	Mass (Da)
	C1F3C4 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 54EB104D59DCA722	FASTA	627	68,501
	10 20 30 40 50 60 MGNLLNSIHS PADIKHFSVP QLEALAQEIR DRLIQSVART GGHIGPNLGV VELTIAMHYV 70 80 90 100 110 120 FDTPQDRFVF DVSHQAYVHK LLTGRANRFD TLRQPGGLNG FMLRSESQHD SYGAGHAGTA 130 140 150 160 170 180 LSAALGMAVA RDIAGGHEHV VALAGDAAFT NGISFEALNN IADQTRRLIV VLNDNEWSID 190 200 210 220 230 240 RNVGAIARYL HKIVTNEHVS QFHDSAARLL KRIGGPAAAN MVRRAEEAAK GMLWPSVLFE 250 260 270 280 290 300 EFGLTYYGPI DGHNLSLLID TFKFLKQQDR PVLLHAITQK GRGFEPALAG QKKFHGLGPF 310 320 330 340 350 360 DPETGETSSS GQPTYSEVFA RSLVKLADQN DKVVAITAAM PNGTGLDHFR PHHPARYFDV 370 380 390 400 410 420 GIAEEHAVIF AAGMATRGFK PYCAIYSTFL QRAFDPIVHD VCLQNLPVVF CMDRGGLSGD 430 440 450 460 470 480 DGPTHHGLFD ISYLRGIPNI VHMVPADEDE LADMMYTAML HDGPSAIRYP RGTGPGHAVK 490 500 510 520 530 540 QQPEALPIGK AKVLHEGEDI AILGLGALLP MAEQIREELA RQGYRAAVIN PRFVKPVDTE 550 560 570 580 590 600 LLAHYADRVT AFLTLEDHVL MGGFGSAVME ELNALGKSTP VVRIGWPDRF IEHGKVDQLR 610 620 ARYGISVEAA MEKLAPYLTR SAPLSVR

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51196 / DSM 11244 / JCM 7670.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001472 Genomic DNA. Translation: ACO33552.1.
RefSeq	YP_002755836.1. NC_012483.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C1F3C4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7698388.
GenomeReviews	Gene locus ACP_2818 in contig CP001472_GR.
KEGG	aca:ACP_2818.
PATRIC	20668875. VBIAciCap40988_2701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RFANEHD.
ProtClustDB	CLSK2319303.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ACIC5

Accession

Primary (citable) accession number: C1F3C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program