C1F3B2 (C1F3B2_ACIC5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
EC=2.1.1.228 HAMAP MF_00605

Alternative name(s):
M1G-methyltransferase HAMAP MF_00605
tRNA [GM37] methyltransferase HAMAP MF_00605

Gene names

Name:	trmD HAMAP MF_00605 EMBL ACO33737.1
Ordered Locus Names:	ACP_2806

Organism

Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670) [Complete proteome] [HAMAP]

Taxonomic identifier

240015 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Acidobacteriales › Acidobacteriaceae › Acidobacterium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605 RuleBase RU003464
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605 S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. RuleBase RU003464
Subunit structure	Homodimer By similarity. HAMAP MF_00605 RuleBase RU003464
Subcellular location	Cytoplasm By similarity HAMAP MF_00605 RuleBase RU003464.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. HAMAP MF_00605 RuleBase RU003464

Ontologies

Keywords
Biological process	tRNA processing HAMAP MF_00605 RuleBase RU003464
Cellular component	Cytoplasm HAMAP MF_00605 RuleBase RU003464
Ligand	S-adenosyl-L-methionine HAMAP MF_00605 RuleBase RU003464
Molecular function	Methyltransferase HAMAP MF_00605 RuleBase RU003464 EMBL ACO33737.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	138 – 143	6	S-adenosyl-L-methionine binding By similarity HAMAP MF_00605
Sites
	Binding site	118	1	S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP MF_00605

Sequences

	Sequence		Length	Mass (Da)
	C1F3B2 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: CB94F599F8853E5C	FASTA	272	30,121
	10 20 30 40 50 60 MRFDILTIFP DFFTSVFSHG VLRRAIAQGQ VSVACHDLRD FTHDRHRTVD DRPFGGGEGM 70 80 90 100 110 120 VLKPEPITEA LESLGLAAKG SRDPQPEAVV LLSAQGMKFT QRVARDLARM ERLVLICGRY 130 140 150 160 170 180 EGVDERVNEL HCDLELSIGD YVLSGGELGA AVIVDATMRL LPGVLGNEAS SAFESFGPTH 190 200 210 220 230 240 EELHGQFSQE AGAPPLSMHG AGGLLDYPHY TRPAEFRGLA VPDVLAGGNH DQIRRWRRER 250 260 270 ALEKTLRNRP DLLEAAELSD EDRRFLRQLQ KG

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001472 Genomic DNA. Translation: ACO33737.1.
RefSeq	YP_002755824.1. NC_012483.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C1F3B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7700971.
GenomeReviews	Gene locus ACP_2806 in contig CP001472_GR.
KEGG	aca:ACP_2806.
PATRIC	20668851. VBIAciCap40988_2689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C1F3B2_ACIC5

Accession

Primary (citable) accession number: C1F3B2

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)