C1EUT6 (MDH_BACC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	BCA_4702

Organism

Bacillus cereus (strain 03BB102) [Complete proteome] [HAMAP]

Taxonomic identifier

572264 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_1000191642
Regions
	Nucleotide binding	12 – 17	6	NAD By similarity
	Nucleotide binding	123 – 125	3	NAD By similarity
Sites
	Active site	180	1	Proton acceptor By similarity
	Binding site	36	1	NAD By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	100	1	NAD By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
Amino acid modifications
	Modified residue	149	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1EUT6 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 615A61C61CA217FD	FASTA	312	33,498
	10 20 30 40 50 60 MTIKRKKVSV IGAGFTGATT AFLLAQKELA DVVLVDIPQL ENPTKGKALD MLEASPVQGF 70 80 90 100 110 120 DANIIGTSDY ADTADSDVVV ITAGIARKPG MSRDDLVATN SKIMKSITRD IAKHSPNAII 130 140 150 160 170 180 VVLTNPVDAM TYSVFKEAGF PKERVIGQSG VLDTARFRTF IAQELNLSVK DITGFVLGGH 190 200 210 220 230 240 GDDMVPLVRY SYAGGIPLET LIPKERLEAI VERTRKGGGE IVGLLGNGSA YYAPAASLVE 250 260 270 280 290 300 MTEAILKDQR RVLPAIAYLE GEYGYSDLYL GVPVILGGNG IEKIIELELL ADEKEALDRS 310 VESVRNVMKV LV

References

[1]	"Genome sequence of Bacillus cereus 03BB102." Dodson R.J., Jackson P., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 03BB102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001407 Genomic DNA. Translation: ACO29854.1.
RefSeq	YP_002751947.1. NC_012472.1.
3D structure databases
ProteinModelPortal	C1EUT6.
SMR	C1EUT6. Positions 5-309.
ModBase	Search...
Protein-protein interaction databases
STRING	572264.BCA_4702.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACO29854; ACO29854; BCA_4702.
GeneID	7692847.
KEGG	bcx:BCA_4702.
PATRIC	18822567. VBIBacCer84800_4597.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	DAMTYVM.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	BCER572264:GH22-4215-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_BACC3

Accession

Primary (citable) accession number: C1EUT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program