C1ER94 (KHSE_BACC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BCA_2032

Organism

Bacillus cereus (strain 03BB102) [Complete proteome] [HAMAP]

Taxonomic identifier

572264 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Homoserine kinase HAMAP MF_00384		PRO_1000134240
Regions
	Nucleotide binding	82 – 92	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	C1ER94 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 0D6F7875E9CBAE1B	FASTA	297	32,098
	10 20 30 40 50 60 MIPLSIRVPA STANVGPGFD SVGIALSLYL HVVVKEKSDK WQVIHSFEDS IPTDDKNLIV 70 80 90 100 110 120 STACKVCPSL SPHIIEVTSN IPLTRGLGSS ASAIVAGIEL ANQLGKLNLT TDQKVQIATN 130 140 150 160 170 180 FEGHPDNVAA SILGGTVIGA LDGKNVSVVR IESKELGVIS LIPNEELNTD ESRSVLPDVF 190 200 210 220 230 240 PFHEAVKASA ISNVLVAALC QKKWEVVGEM MEMDHFHEPY RLELVPLLPS IRKCAKEFGA 250 260 270 280 290 YGTALSGAGP SIFILTPYEK RQEIAEQLAR VFTSMKVCEL EIDHRGITVN KKEHIGL

References

[1]	"Genome sequence of Bacillus cereus 03BB102." Dodson R.J., Jackson P., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 03BB102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001407 Genomic DNA. Translation: ACO26617.1.
RefSeq	YP_002749306.1. NC_012472.1.
3D structure databases
ProteinModelPortal	C1ER94.
ModBase	Search...
Protein-protein interaction databases
STRING	C1ER94.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000113955; EBBACP00000109164; EBBACG00000114475.
GeneID	7692475.
GenomeReviews	Gene locus BCA_2032 in contig CP001407_GR.
KEGG	bcx:BCA_2032.
PATRIC	18817183. VBIBacCer84800_1908.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000000874.
OMA	DRLHQPY.
ProtClustDB	PRK01212.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACC3

Accession

Primary (citable) accession number: C1ER94

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program