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C1EQZ2 (BIOD_BACC3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:BCA_4231
OrganismBacillus cereus (strain 03BB102) [Complete proteome] [HAMAP]
Taxonomic identifier572264 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 242242ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000133202

Regions

Nucleotide binding12 – 176ATP By similarity
Nucleotide binding112 – 1154ATP By similarity

Sites

Metal binding121Magnesium 1 By similarity
Metal binding161Magnesium 2 By similarity
Metal binding511Magnesium 2 By similarity
Metal binding1121Magnesium 2 By similarity
Binding site411Substrate By similarity
Binding site511ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C1EQZ2 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 0496BD9F34F3A3D4

FASTA24226,307
        10         20         30         40         50         60 
MSGFFITATD TEVGKTVVAG ALAGVFRELG YNIGVYKPLQ SGHVASNPEG DAARLKVLSG 

        70         80         90        100        110        120 
VPTKEDEICP YSIEEPLAPR LAMKRAGRAV TLKDIIHHYN ERLKEFNSLF VEGAGGLAVP 

       130        140        150        160        170        180 
YTEDALVIDF AKELQLPLIV VARPTLGTVN HTVLTIAYAK AHGLTVAGVI LSGCKECEME 

       190        200        210        220        230        240 
RVQENKVMIE ELSGVPVLGL LPFFEGEFTK KEVLESAKEY IMISKLEEFI RNESTVAHTS 


SN

« Hide

References

[1]"Genome sequence of Bacillus cereus 03BB102."
Dodson R.J., Jackson P., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D.
Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 03BB102.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001407 Genomic DNA. Translation: ACO27331.1.
RefSeqYP_002751482.1. NC_012472.1.

3D structure databases

ProteinModelPortalC1EQZ2.
ModBaseSearch...

Protein-protein interaction databases

STRINGC1EQZ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000117960; EBBACP00000114574; EBBACG00000113431.
GeneID7689941.
GenomeReviewsGene locus BCA_4231 in contig CP001407_GR.
KEGGbcx:BCA_4231.
PATRIC18821613. VBIBacCer84800_4122.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000001962.
OMAANCVDPN.
ProtClustDBPRK00090.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_BACC3
AccessionPrimary (citable) accession number: C1EQZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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