C1EPL6 (ILVD_BACC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BCA_1859

Organism

Bacillus cereus (strain 03BB102) [Complete proteome] [HAMAP]

Taxonomic identifier

572264 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP-Rule MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP-Rule MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP-Rule MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Dihydroxy-acid dehydratase HAMAP-Rule MF_00012		PRO_1000116499
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	C1EPL6 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 39AACA4DC2C3C30B	FASTA	557	59,992
	10 20 30 40 50 60 MRSDMIKKGF DKAPHRSLLK ATGLKDEDFD KPFIAICNSF IEIIPGHKHL NEFGKLVKEA 70 80 90 100 110 120 VRAAGMVPFE FNTIGVDDGI AMGHIGMRYS LPSREIIADS VETVVNAHWF DGMICIPNCD 130 140 150 160 170 180 KITPGMMMAA LRINIPTVFV SGGPMAAGKT SKGDVVDLSS VFEGVGAYQS GKISEEELKD 190 200 210 220 230 240 IEDHGCPSCG SCSGMFTANS MNCLCEVLGL ALPGNGSILA IDPRREELIK QAAEKLKILI 250 260 270 280 290 300 ERDIKPRDIV TEEAIDDAFA LDMAMGGSTN TVLHTLALAQ EAGLDYDMNR IDAVSRRVPH 310 320 330 340 350 360 LCKVSPASNW HMEDIDRAGG ISAILKEMSR KEGVLHLDRI TATGQTLREN IAHAEIKDKE 370 380 390 400 410 420 VIHSLENPHS EEGGLRILKG NLAKDGAVIK SGATEVKRFE GPCVIFNSQD EALAGIMLGK 430 440 450 460 470 480 VKKGDVVVIR YEGPRGGPGM PEMLAPTSAI AGMGLGADVA LLTDGRFSGA SRGISVGHIS 490 500 510 520 530 540 PEAAAGGTIA LLEQGDIVCI DVEERLLEVR VSDEELDKRK KEWKRPEPKV KTGWLGRYAQ 550 MVTSANTGAV LKIPNFD

References

[1]	"Genome sequence of Bacillus cereus 03BB102." Dodson R.J., Jackson P., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D. Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 03BB102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001407 Genomic DNA. Translation: ACO26410.1.
RefSeq	YP_002749134.1. NC_012472.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	572264.BCA_1859.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACO26410; ACO26410; BCA_1859.
GeneID	7689742.
KEGG	bcx:BCA_1859.
PATRIC	18816839. VBIBacCer84800_1736.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HOG000173155.
KO	K01687.
OMA	QGRNMAG.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	BCER572264:GH22-771-MONOMER.
UniPathway	UPA00047; UER00057. UPA00049; UER00061.
Family and domain databases
HAMAP	MF_00012. IlvD.
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. PTHR21000. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACC3

Accession

Primary (citable) accession number: C1EPL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program