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C1DSU8

- GLND_AZOVD

UniProt

C1DSU8 - GLND_AZOVD

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, Avin_39010
Organism
Azotobacter vinelandii (strain DJ / ATCC BAA-1303)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciAVIN322710:GJ0M-3907-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Avin_39010
OrganismiAzotobacter vinelandii (strain DJ / ATCC BAA-1303)
Taxonomic identifieri322710 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter
ProteomesiUP000002424: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 899899Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000204797Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi322710.Avin_39010.

Structurei

3D structure databases

ProteinModelPortaliC1DSU8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini486 – 590105HD
Add
BLAST
Domaini706 – 78984ACT 1
Add
BLAST
Domaini816 – 89984ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotation
Add
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1DSU8-1 [UniParc]FASTAAdd to Basket

« Hide

MPQVDPDLFD PGQFQAELAL KSSPIPAYKK ALRCAREVLD ARFQEGRDIR    50
RLIEDRAWFV DQILALAWNR FDWSEDADIA LIAVGGYGRG ELHPYSDIDL 100
LILMDGADHE VFREPIEGFL TLLWDIGLEV GQSVRSLAEC AEEAQADLTV 150
ITNLMESRTI AGPEHLRQRM QEVTSAQRMW PSRAFFLAKR DEQKTRHARY 200
NDTEYNLEPN VKGSPGGLRD IQTLLWIARR QFGTINLHAM VGQGFLLESE 250
YTLLASSQEF LWKVRYALHM LAGRAEDRLL FDLQRQIAGL LGYEDSDAKL 300
AVERFMQKYY RVVLGIAELT ELVFQHFEEV ILPGDAAGRV EPLNERFQVR 350
DGYLEVTHAG VFQETPSALL EIFVLLARRP EIRGVRADTI RLLRDHRYLI 400
DDAFRRDPHN TGLFIELFKS RQGIHRNLRR MNRYGILGRY LPEFGHIVGQ 450
MQHDLFHIYT VDAHTLNLIK NLRKLFWPEL AEKYPLASKL IEKLPKPELI 500
YLAGLYHDIG KGRGGDHSEL GAADALAFCQ RHDLPAMDTQ LIVWLVRNHL 550
LMSTTAQRKD LSDPQVIFDF AQKVRDQTYL DYLYVLTVAD INATNPTLWN 600
SWRASLLRQL YTETKHALRR GLEQPVGREE QIRQTQKAAL DILVRSGTDP 650
DDAEHLWTQL GDDYFLRHTS SDIAWHTEAI LQHPSSGGPL VLIKETTQRE 700
FEGATQIFIY APDQHDFFAV TVAAMDQLNL SIHDARVITS TSQFTLDTYI 750
VLDADGGSIG NNPARIQEIR QGLVEALRNP ADYPTIIQRR VPRQLKHFAF 800
APQVTIQNDA LRPVTILEII APDRPGLLAR IGKIFLDFDL SLQNAKIATL 850
GERVEDVFFV TDAHNQPLSD PELCARLQLA IAEQLADGDS YIQPSRISI 899
Length:899
Mass (Da):102,580
Last modified:May 26, 2009 - v1
Checksum:i96AF63BC5EDE3C9D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001157 Genomic DNA. Translation: ACO80041.1.
RefSeqiYP_002801016.1. NC_012560.1.

Genome annotation databases

EnsemblBacteriaiACO80041; ACO80041; Avin_39010.
GeneIDi7762790.
KEGGiavn:Avin_39010.
PATRICi21033848. VBIAzoVin72790_3666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001157 Genomic DNA. Translation: ACO80041.1 .
RefSeqi YP_002801016.1. NC_012560.1.

3D structure databases

ProteinModelPortali C1DSU8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 322710.Avin_39010.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO80041 ; ACO80041 ; Avin_39010 .
GeneIDi 7762790.
KEGGi avn:Avin_39010.
PATRICi 21033848. VBIAzoVin72790_3666.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci AVIN322710:GJ0M-3907-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DJ / ATCC BAA-1303.

Entry informationi

Entry nameiGLND_AZOVD
AccessioniPrimary (citable) accession number: C1DSU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi