C1DRI9 (ACDH2_AZOVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetaldehyde dehydrogenase 2
EC=1.2.1.10

Alternative name(s):
Acetaldehyde dehydrogenase [acetylating] 2

Gene names

Name:	mhpF
Ordered Locus Names:	Avin_15120

Organism

Azotobacter vinelandii (strain DJ / ATCC BAA-1303) [Complete proteome] [HAMAP]

Taxonomic identifier

322710 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter group › Azotobacter › Azotobacter vinelandii

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Is the final enzyme in the meta-cleavage pathway for the degradation of aromatic compounds By similarity. HAMAP-Rule MF_01657
Catalytic activity	Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH. HAMAP-Rule MF_01657
Sequence similarities	Belongs to the acetaldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	NAD binding Inferred from electronic annotation. Source: HAMAP acetaldehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Acetaldehyde dehydrogenase 2 HAMAP-Rule MF_01657		PRO_0000387620
Regions
	Nucleotide binding	11 – 14	4	NAD By similarity
	Nucleotide binding	162 – 170	9	NAD By similarity
Sites
	Active site	131	1	Acyl-thioester intermediate By similarity
	Binding site	289	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	C1DRI9 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: B14C3AAC1683824A	FASTA	311	32,656
	10 20 30 40 50 60 MKKLKVAIVG SGNIGTDLMI KILRHGQHLE MGALVGIDPD SDGLARAARL GVATTAEGVE 70 80 90 100 110 120 GLARLPGFGE IDFVFDATSA AAHVKNDPFL RGLRPGLRLI DLTPAAVGPY CVPVVNLEQN 130 140 150 160 170 180 LREPNVNMVT CGGQATIPMV AAVSRVARVH YAEIVASIAS RSAGPGTRAN IDEFTETTSK 190 200 210 220 230 240 AIEAIGGARK GKAIIVLNPA EPPLIMRDTV YVLSAPADQA RVEASLAEMA QAVQGYVPGY 250 260 270 280 290 300 RLKQRVQFDE IPDAAPLNIP GLGRLSGLKT SVFLEVEGAA HYLPAYAGNL DIMTSAALAT 310 AERMAQSMLN A

References

[1]

"Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes." Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G., Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L., Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K., Hernandez J.A., Houmiel K., Imperial J. , Kennedy C., Larson T.J., Latreille P., Ligon L.S., Lu J., Maerk M., Miller N.M., Norton S., O'Carroll I.P., Paulsen I., Raulfs E.C., Roemer R., Rosser J., Segura D., Slater S., Stricklin S.L., Studholme D.J., Sun J., Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H., Dean D.R., Dixon R., Wood D. J. Bacteriol. 191:4534-4545(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DJ / ATCC BAA-1303.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001157 Genomic DNA. Translation: ACO77727.1.
RefSeq	YP_002798702.1. NC_012560.1.
3D structure databases
ProteinModelPortal	C1DRI9.
SMR	C1DRI9. Positions 2-311.
ModBase	Search...
Protein-protein interaction databases
STRING	322710.Avin_15120.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACO77727; ACO77727; Avin_15120.
GeneID	7760447.
KEGG	avn:Avin_15120.
PATRIC	21029311. VBIAzoVin72790_1438.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4569.
HOGENOM	HOG000052149.
OMA	ASRPINI.
ProtClustDB	PRK08300.
Enzyme and pathway databases
BioCyc	AVIN322710:GJ0M-1513-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01657. Ac_ald_DH_ac.
InterPro	IPR003361. Acetaldehyde_dehydrogenase. IPR015426. Acetylaldehyde_DH_C. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. [Graphical view]
PANTHER	PTHR21123. PTHR21123. 1 hit.
Pfam	PF09290. AcetDehyd-dimer. 1 hit. PF01118. Semialdhyde_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015689. Actaldh_dh_actl. 1 hit.
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACDH2_AZOVD

Accession

Primary (citable) accession number: C1DRI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program