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C1DPH5

- SYI_AZOVD

UniProt

C1DPH5 - SYI_AZOVD

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Avin_11780
Organism
Azotobacter vinelandii (strain DJ / ATCC BAA-1303)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylate By similarity
Binding sitei611 – 6111ATP By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity
Metal bindingi929 – 9291Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAVIN322710:GJ0M-1174-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Avin_11780
OrganismiAzotobacter vinelandii (strain DJ / ATCC BAA-1303)
Taxonomic identifieri322710 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter
ProteomesiUP000002424: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligaseUniRule annotationPRO_1000216232Add
BLAST

Proteomic databases

PRIDEiC1DPH5.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi322710.Avin_11780.

Structurei

3D structure databases

ProteinModelPortaliC1DPH5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi608 – 6125"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1DPH5-1 [UniParc]FASTAAdd to Basket

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MTDYKATLNL PETAFPMKAG LPQREPETLR HWNSIGLYRK LRQIGEGRQK    50
FILHDGPPYA NGNIHIGHAV NKILKDMIVR SKTLAGFDAP YVPGWDCHGL 100
PIEHKVETTF GKNQPADLTR ERCRAYAAEQ VEGQKADFIR LGVLGDWENP 150
YKTMDFANEA GEIRALAKMV EGGFVFKGLK PVNWCFDCGS ALAEAEVEYQ 200
DKKSDAIDVF FPVGDADKLA AAFGLAQLDK PAGIVIWTTT PWTIPANQAL 250
NVHPDFIYAL VDCGDRLLLL AEELVEGCLA RYGLRGQVLA TAKGEALELI 300
RFRHPFYERF SPVYLADYVG LDAGTGIVHS APAYGEDDFR SCKRYGMSND 350
EILSPVQSNG VYVESLPFFG GQFIWKANPN IVAKLAEVGC LLKHETISHS 400
YMHCWRHKTP LIYRATAQWF VGMDSKAKDG TTLRERALAA IEQTRFVPAW 450
GQARLHGMIA GRPDWCISRQ RNWGVPIPFF LHKESGELHP RTVELMEEVA 500
RRVEQKGIEA WFRLDPAELL GAEADQYDKI ADTLDVWFDS GTTHWHVMRG 550
SHPMGHDQGP RADLYLEGSD QHRGWFHSSL LTGAAIDGHA PYRGLLTHGF 600
TVDENGRKMS KSLGNVIAPQ EITDSMGADI LRLWVSATDY SGEMAVSKQI 650
LQRSADAYRR IRNTARFLLA NLSGFDPAKD LLAAEDMLAL DRWAVDRALL 700
LQREVEEAFD SYRFWVVYQK VHNFCVQELG GFYLDIIKDR QYTCAADSVA 750
RRSCQSAMYH IGEALVRWIA PILAFTAEEI WQYLPGERNE SVMLNTWYQG 800
FAELPDGFAL DRAFWEQVMA VKAAVNKELE NRRNAKAIGG NLQAEVVLFA 850
EPALAGRLAL LGDELRFVLI TSAAQVQPLG AAPADAVDTE VAGLRIQVSK 900
SVHTKCARCW HHREDVGLDP AHPEICGRCV ENIQGEGEVR QYA 943
Length:943
Mass (Da):105,564
Last modified:May 26, 2009 - v1
Checksum:i9F18D38C266AE585
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001157 Genomic DNA. Translation: ACO77407.1.
RefSeqiYP_002798382.1. NC_012560.1.

Genome annotation databases

EnsemblBacteriaiACO77407; ACO77407; Avin_11780.
GeneIDi7760120.
KEGGiavn:Avin_11780.
PATRICi21028633. VBIAzoVin72790_1108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001157 Genomic DNA. Translation: ACO77407.1 .
RefSeqi YP_002798382.1. NC_012560.1.

3D structure databases

ProteinModelPortali C1DPH5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 322710.Avin_11780.

Proteomic databases

PRIDEi C1DPH5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO77407 ; ACO77407 ; Avin_11780 .
GeneIDi 7760120.
KEGGi avn:Avin_11780.
PATRICi 21028633. VBIAzoVin72790_1108.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AVIN322710:GJ0M-1174-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DJ / ATCC BAA-1303.

Entry informationi

Entry nameiSYI_AZOVD
AccessioniPrimary (citable) accession number: C1DPH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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