Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Azotobacter vinelandii (strain DJ / ATCC BAA-1303)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
Binding sitei611 – 6111ATPUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAVIN322710:GJ0M-1174-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Avin_11780
OrganismiAzotobacter vinelandii (strain DJ / ATCC BAA-1303)
Taxonomic identifieri322710 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter
ProteomesiUP000002424 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000216232Add
BLAST

Proteomic databases

PRIDEiC1DPH5.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi322710.Avin_11780.

Structurei

3D structure databases

ProteinModelPortaliC1DPH5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi608 – 6125"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C1DPH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKATLNL PETAFPMKAG LPQREPETLR HWNSIGLYRK LRQIGEGRQK
60 70 80 90 100
FILHDGPPYA NGNIHIGHAV NKILKDMIVR SKTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVETTF GKNQPADLTR ERCRAYAAEQ VEGQKADFIR LGVLGDWENP
160 170 180 190 200
YKTMDFANEA GEIRALAKMV EGGFVFKGLK PVNWCFDCGS ALAEAEVEYQ
210 220 230 240 250
DKKSDAIDVF FPVGDADKLA AAFGLAQLDK PAGIVIWTTT PWTIPANQAL
260 270 280 290 300
NVHPDFIYAL VDCGDRLLLL AEELVEGCLA RYGLRGQVLA TAKGEALELI
310 320 330 340 350
RFRHPFYERF SPVYLADYVG LDAGTGIVHS APAYGEDDFR SCKRYGMSND
360 370 380 390 400
EILSPVQSNG VYVESLPFFG GQFIWKANPN IVAKLAEVGC LLKHETISHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF VGMDSKAKDG TTLRERALAA IEQTRFVPAW
460 470 480 490 500
GQARLHGMIA GRPDWCISRQ RNWGVPIPFF LHKESGELHP RTVELMEEVA
510 520 530 540 550
RRVEQKGIEA WFRLDPAELL GAEADQYDKI ADTLDVWFDS GTTHWHVMRG
560 570 580 590 600
SHPMGHDQGP RADLYLEGSD QHRGWFHSSL LTGAAIDGHA PYRGLLTHGF
610 620 630 640 650
TVDENGRKMS KSLGNVIAPQ EITDSMGADI LRLWVSATDY SGEMAVSKQI
660 670 680 690 700
LQRSADAYRR IRNTARFLLA NLSGFDPAKD LLAAEDMLAL DRWAVDRALL
710 720 730 740 750
LQREVEEAFD SYRFWVVYQK VHNFCVQELG GFYLDIIKDR QYTCAADSVA
760 770 780 790 800
RRSCQSAMYH IGEALVRWIA PILAFTAEEI WQYLPGERNE SVMLNTWYQG
810 820 830 840 850
FAELPDGFAL DRAFWEQVMA VKAAVNKELE NRRNAKAIGG NLQAEVVLFA
860 870 880 890 900
EPALAGRLAL LGDELRFVLI TSAAQVQPLG AAPADAVDTE VAGLRIQVSK
910 920 930 940
SVHTKCARCW HHREDVGLDP AHPEICGRCV ENIQGEGEVR QYA
Length:943
Mass (Da):105,564
Last modified:May 26, 2009 - v1
Checksum:i9F18D38C266AE585
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001157 Genomic DNA. Translation: ACO77407.1.
RefSeqiWP_012699827.1. NC_012560.1.

Genome annotation databases

EnsemblBacteriaiACO77407; ACO77407; Avin_11780.
KEGGiavn:Avin_11780.
PATRICi21028633. VBIAzoVin72790_1108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001157 Genomic DNA. Translation: ACO77407.1.
RefSeqiWP_012699827.1. NC_012560.1.

3D structure databases

ProteinModelPortaliC1DPH5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi322710.Avin_11780.

Proteomic databases

PRIDEiC1DPH5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACO77407; ACO77407; Avin_11780.
KEGGiavn:Avin_11780.
PATRICi21028633. VBIAzoVin72790_1108.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciAVIN322710:GJ0M-1174-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DJ / ATCC BAA-1303.

Entry informationi

Entry nameiSYI_AZOVD
AccessioniPrimary (citable) accession number: C1DPH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: July 22, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.