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C1DMR1

- LIPA_AZOVD

UniProt

C1DMR1 - LIPA_AZOVD

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Protein

Lipoyl synthase

Gene
lipA, Avin_08460
Organism
Azotobacter vinelandii (strain DJ / ATCC BAA-1303)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi102 – 1021Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi105 – 1051Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAVIN322710:GJ0M-853-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Avin_08460
OrganismiAzotobacter vinelandii (strain DJ / ATCC BAA-1303)
Taxonomic identifieri322710 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter
ProteomesiUP000002424: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Lipoyl synthaseUniRule annotationPRO_1000204142Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi322710.Avin_08460.

Structurei

3D structure databases

ProteinModelPortaliC1DMR1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

C1DMR1-1 [UniParc]FASTAAdd to Basket

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MSTTETTGKS PAKVETGVKL RGAEKVARIP VKVIPTEELP RKPDWIRVRM    50
PASPEVERIK QLLRQHKLHS VCEEASCPNL GECFAGGTAT FMIMGDICTR 100
RCPFCDVGHG RPNPLDPDEP KNLAVAVADL RLKYVVITSV DRDDLRDGGA 150
QHFADCLREI RRLSPGTQLE TLVPDYRGRM EIALDITAAE PPDVFNHNLE 200
TVPRLYKASR PGADFEWSLD LLEAFKKRVP AVPTKSGLML GLGETDEEVI 250
EVMKRMREHE IDMLTLGQYL QPSRNHLAVQ RFVHPDTFAW FAEEGERMGF 300
KNVTSGPLVR SSYHADQQAH GAKGD 325
Length:325
Mass (Da):36,346
Last modified:May 26, 2009 - v1
Checksum:iB3FF680CEE76D53E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001157 Genomic DNA. Translation: ACO77091.1.
RefSeqiYP_002798066.1. NC_012560.1.

Genome annotation databases

EnsemblBacteriaiACO77091; ACO77091; Avin_08460.
GeneIDi7759800.
KEGGiavn:Avin_08460.
PATRICi21028005. VBIAzoVin72790_0795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001157 Genomic DNA. Translation: ACO77091.1 .
RefSeqi YP_002798066.1. NC_012560.1.

3D structure databases

ProteinModelPortali C1DMR1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 322710.Avin_08460.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO77091 ; ACO77091 ; Avin_08460 .
GeneIDi 7759800.
KEGGi avn:Avin_08460.
PATRICi 21028005. VBIAzoVin72790_0795.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci AVIN322710:GJ0M-853-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DJ / ATCC BAA-1303.

Entry informationi

Entry nameiLIPA_AZOVD
AccessioniPrimary (citable) accession number: C1DMR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 26, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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