C1DI80 (C1DI80_AZOVD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutathione synthetase HAMAP-Rule MF_00162
EC=6.3.2.3 HAMAP-Rule MF_00162

Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162

Gene names

Name:	gshB HAMAP-Rule MF_00162 EMBL ACO76577.1
Ordered Locus Names:	Avin_03170

Organism

Azotobacter vinelandii (strain DJ / ATCC BAA-1303) [Complete proteome] [HAMAP] EMBL ACO76577.1

Taxonomic identifier

322710 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter group › Azotobacter › Azotobacter vinelandii

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162
Cofactor	Binds (magnesium or manganese,ion) per subunit By similarity. HAMAP-Rule MF_00162
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162 Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Ontologies

Keywords
Biological process	Glutathione biosynthesis HAMAP-Rule MF_00162
Ligand	ATP-binding HAMAP-Rule MF_00162 Magnesium HAMAP-Rule MF_00162 Manganese HAMAP-Rule MF_00162 Metal-binding HAMAP-Rule MF_00162 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00162
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	126 – 311	186	ATP-grasp By similarity HAMAP-Rule MF_00162
	Nucleotide binding	152 – 208	57	ATP By similarity HAMAP-Rule MF_00162
Sites
	Metal binding	282	1	Magnesium or manganese By similarity HAMAP-Rule MF_00162
	Metal binding	284	1	Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

	Sequence		Length	Mass (Da)
	C1DI80 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 9E573920CD539366	FASTA	318	35,714
	10 20 30 40 50 60 MSIRLGIVMD PIAQISFKKD SSLAMLQSAQ ARGWSLHYME PQDLYQHGNE ARARMRPLRV 70 80 90 100 110 120 FDDPARWFEL QEENDTPLGD LDVILMRKDP PFNSEYVYAT YLLELAEKAG CLVVNRPQSL 130 140 150 160 170 180 RDCNEKFFAT QFPQFTPPTL VSRRADILRE FAAEHGDVIL KPLDEMGGAS VFRHREGDPN 190 200 210 220 230 240 LSVILEVLTE HGARQIMAQR YLPAIKDGDK RILMVDGEPV PYCLARIPAP GETRGNLAAG 250 260 270 280 290 300 GRGVAQPLTE RDREIAAHVG PELRRRGLLF VGLDVIGEHL TEINVTSPTC IREIDNAYDT 310 RIGERLMDAI AQRLAARG

References

[1]

"Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes." Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G., Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L., Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K., Hernandez J.A., Houmiel K., Imperial J. , Kennedy C., Larson T.J., Latreille P., Ligon L.S., Lu J., Maerk M., Miller N.M., Norton S., O'Carroll I.P., Paulsen I., Raulfs E.C., Roemer R., Rosser J., Segura D., Slater S., Stricklin S.L., Studholme D.J., Sun J., Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H., Dean D.R., Dixon R., Wood D. J. Bacteriol. 191:4534-4545(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DJ / ATCC BAA-1303.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001157 Genomic DNA. Translation: ACO76577.1.
RefSeq	YP_002797552.1. NC_012560.1.
3D structure databases
ProteinModelPortal	C1DI80.
SMR	C1DI80. Positions 3-314.
ModBase	Search...
Protein-protein interaction databases
STRING	322710.Avin_03170.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACO76577; ACO76577; Avin_03170.
GeneID	7759277.
KEGG	avn:Avin_03170.
PATRIC	21026988. VBIAzoVin72790_0296.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0189.
HOGENOM	HOG000265022.
KO	K01920.
OMA	WMRKDPP.
ProtClustDB	PRK05246.
Enzyme and pathway databases
BioCyc	AVIN322710:GJ0M-314-MONOMER.
UniPathway	UPA00142; UER00210.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00162. GSH_S.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C1DI80_AZOVD

Accession

Primary (citable) accession number: C1DI80

Entry history

Integrated into UniProtKB/TrEMBL:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)